Ahijado Guzmán, RubénGonzález Rubio, GuillermoIzquierdo, Jesús G.Bañares Morcillo, LuisLópez-Montero, IvánCalzado Martín, AliciaCalleja, MontserratTardajos Rodríguez, GloriaGuerrero Martínez, Andrés2023-06-182023-06-1820162470-134310.1021/acsomega.6b00184https://hdl.handle.net/20.500.14352/23742The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (ERC grant agreement n° 338133)The search for efficient plasmonic photothermal therapies using nonharmful pulse laser irradiation at the near-infrared (NIR) is fundamental for biomedical cancer research. Therefore, the development of novel assembled plasmonic gold nanostructures with the aim of reducing the applied laser power density to a minimum through hot-spot-mediated cell photothermolysis is an ongoing challenge. We demonstrate that gold nanorods (Au NRs) functionalized at their tips with a pH-sensitive ligand assemble into oligomers within cell lysosomes through hydrogen-bonding attractive interactions. The unique intracellular features of the plasmonic oligomers allow us to significantly reduce the femtosecond laser power density and Au NR dose while still achieving excellent cell killing rates. The formation of gold tip-to-tip oligomers with longitudinal localized surface plasmon resonance bands at the NIR, obtained from low-aspect-ratio Au NRs close in resonance with 800 nm Ti:sapphire 90 fs laser pulses, was found to be the key parameter for realizing the enhanced plasmonic photothermal therapy.engjournal articlehttps://doi.org/10.1021/acsomega.6b00184open accessDrug delivery systemsLaser radiationNanostructuresPharmacologyMaterialesQuímica física (Química)3312 Tecnología de Materiales