Martínez Caballero, María AranzazuMartínez Larrañaga, María RosaCastellano Santos, Víctor JesúsMartínez Caballero, MartaAres Lombán, IrmaRomero Martínez, Manuel AlejandroAnadón Navarro, Arturo Ramón2024-02-022024-02-022013Martínez MA, Martínez-Larrañaga MR, Castellano V, Martínez M, Ares I, Romero A, Anadón A. Effect of natamycin on cytochrome P450 enzymes in rats. Food Chem Toxicol. 2013 Dec;62:281-4. doi: 10.1016/j.fct.2013.08.075. Epub 2013 Aug 31. PMID: 240014390278-691510.1016/j.fct.2013.08.075https://hdl.handle.net/20.500.14352/98184Natamycin is a polyene macrolide antibiotic widely used in the food industry as a feed additive to prevent mold contamination of foods. There are many contradictory results on the genotoxic effects of macrolides which could suggest a potential risk for humans. In the present study, the effects of natamycin on the activities of some drug metabolizing enzymes in rat liver microsomes were determined in vivo. Rats were treated orally with natamycin at doses of 0.3, 1, 3 and 10 mg/kg body weight (bw)/day for 6 days. Determinations of cytochrome P450 (CYP) enzyme activities were carried out in hepatic microsomes isolated from rats treated. The activities of CYP2E1, CYP1A1/2 CYP2B1/2 and CYP4A1/2 enzymes significantly decreased after treatment with 1, 3 and 10 mg/kg bw/day, in a dose-dependent manner as compared to control. This effect was not observed after natamycin treatment at dose of 0.3 mg/kg bw/day. Our results suggest that natamycin may not potentiate the toxicity of many xenobiotics via metabolic activation and/or accumulation of reactive metabolites but also might affect the clearance of other xenobiotics detoxified by the studied CYP enzymes.engjournal articlehttps://doi.org/10.1016/j.fct.2013.08.07524001439restricted access615.9NatamycinCytochrome P450 enzymesHepatic microsomesRatsCiencias Biomédicas3214 Toxicología