Protective effects of 17-β-oestradiol and phytoestrogen on age-induced oxidative stress and inhibition of surfactant synthesis in rat type II pneumocytes

Rancán, LisaPuig, AngelaBalibrea, José M.Paredes Royano, Sergio DamiánGarcía Martín, M. CruzJiménez Roldán, LuisFernández-Tresguerres Hernández, Jesús ÁngelVara Ameigeiras, Elena María2025-01-152025-01-152020Rancan L, Puig A, Balibrea JM, Paredes SD, García C, Jiménez L, Fernández-Tresguerres JA, Vara E. Protective effects of 17-β-oestradiol and phytoestrogen on age-induced oxidative stress and inhibition of surfactant synthesis in rat type II pneumocytes. Int J Food Sci Nutr. 2021 Feb;72(1):26-36. doi: 10.1080/09637486.2020.17570440963-748610.1080/09637486.2020.1757044https://hdl.handle.net/20.500.14352/114467Epidemiological data suggest protective effects of oestrogen and phytoestrogen on lung tissue. This study aimed to elucidate the role of 17-β-oestradiol and phytoestrogen in age-related inhibition of surfactant synthesis and oxidative stress in rat type II pneumocytes. Forty male and 66 female Wistar rats were used. Female rats were randomly kept intact or ovariectomized at age 12 months. At age 22 months, ovariectomized rats received 17-β-oestradiol, soy extract, or no treatment. Oxidative stress markers CO, NO, cGMP and lipid peroxide (LPO), antioxidant enzymes and phosphatidylcholine (PC) were measured in cultured type II pneumocytes isolated at ages 2, 14, 18, 22 and 24 months. Old, male and ovariectomized rats showed significantly higher CO, NO, cGMP and LPO and lower PC content and antioxidant enzymes. 17-β-oestradiol and phytoestrogen significantly reversed these effects. In conclusion, aging and oestrogen deprivation decreased PC synthesis and altered the redox status in type II pneumocytes, which were partially restored by 17-β-oestradiol or soy supplementation.engProtective effects of 17-β-oestradiol and phytoestrogen on age-induced oxidative stress and inhibition of surfactant synthesis in rat type II pneumocytesjournal article1465-3478https://doi.org/10.1080/09637486.2020.175704432314935https://www.tandfonline.com/doi/10.1080/09637486.2020.1757044?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmedhttps://pubmed.ncbi.nlm.nih.gov/32314935/restricted access577.117-β-OestradiolAgingOxidative stressPhytoestrogenSurfactantType ii pneumocytes.Ciencias BiomédicasMedicinaBioquímica (Medicina)24 Ciencias de la Vida2403 Bioquímica