Vargas, EvaTeymourian, HazhirTehrani, FarshadEksin, EceSánchez Tirado, EstherWarren, PaulErdem, ArzumDassau, EyalWang, Joseph2024-01-252024-01-252019Vargas E, Teymourian H, Tehrani F, Eksin E, Sánchez-Tirado E, Warren P, Erdem A, Dassau E, Wang J. Enzymatic/Immunoassay Dual-Biomarker Sensing Chip: Towards Decentralized Insulin/Glucose Detection. Angew Chem Int Ed. 2019 May 6; 58(19):6376-791433-785110.1002/anie.201902664https://hdl.handle.net/20.500.14352/95345Performing bioassay formats based on enzyme and antibody recognition reactions with a single detection chip remains an unmet challenge owing to the different requirements of such bioassays. Herein, we describe a dual-marker biosensor chip, integrating enzyme and antibody-based assays for simultaneous electrochemical measurements of insulin (I) and glucose (G). Simultaneous G/I sensing has been realized by addressing key fabrication and operational challenges associated with the different assay requirements and surface chemistry. The I immunosensor relies on a peroxidase-labeled sandwich immunoassay, while G is monitored through reaction with glucose oxidase. The dual diabetes biomarker chip offers selective and reproducible detection of picomolar I and millimolar G concentrations in a single microliter sample droplet within less than 30 min, including direct measurements in whole blood and saliva samples. The resulting integrated enzymatic-immunoassay biosensor chip opens a new realm in point-of-care multiplexed biomarker detection.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal article1521-3773https://doi.org/10.1002/anie.201902664https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201902664restricted access543BiosensorsDiabetes monitoringDual-marker detectionElectrochemistryImmunoassaysQuímica analítica (Química)2301 Química Analítica2301.04 Análisis Electroquímico