Menéndez-Méndez, AidaPaniagua-Herranz, LucíaOlivos Ore, Luis AlcidesGómez Villafuertes, María RosaRodríguez Artalejo, AntonioPérez Sen, RaquelGarcía Delicado, EsmerildaOrtega De La O, Felipe2024-01-232024-01-232021-12-172666-166710.1016/j.xpro.2021.100964https://hdl.handle.net/20.500.14352/94829Low-density cell culture of the postnatal cerebellum, combined with live imaging and single-cell tracking, allows the behavior of postnatal cerebellar neural stem cells (NSCs) and their progeny to be monitored. Cultured cerebellar NSCs maintain their neurogenic nature giving rise, in the same relative proportions that exist in vivo, to the neuronal progeny generated by the three postnatal cerebellar neurogenic niches. This protocol describes the identification of the nature of the progeny through both post-imaging immunocytochemistry and patch-clamp recordings. For complete details on the use and execution of this protocol, please refer to Paniagua-Herranz et al. (2020b).engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cellsCombinación de cultivo celular de baja densidad, seguimiento unicelular y patch-clamp para monitorizar el comportamiento de células madre neurales cerebelosas murinas postnatalesjournal articlehttps://www.sciencedirect.com/science/article/pii/S266616672100670534841278https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605430/open access6Cell BiologyCell cultureDevelopmental biologyMicroscopyNeuroscience;Single CellStem CellsCiencias Biomédicas32 Ciencias Médicas