Person: Pérez García, Lucas
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First Name
Lucas
Last Name
Pérez García
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Físicas
Department
Física de Materiales
Area
Física Aplicada
Identifiers
2 results
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Item Flexible metallic core–shell nanostructured electrodes for neural interfacing(Scientific Reports, 2024) Rodilla González, Beatriz Loreto; Arché-Nuñez, Ana; Ruiz-Gómez, Sandra; Domínguez-Bajo, Ana; Fernández-González, Claudia; Guillén-Colomer, Clara; González-Mayorga, Ankor; Rodríguez-Díez, Noelia; Camarero, Julio; Miranda, Rodolfo; López-Dolado, Elisa; Ocón, Pilar; Serrano, María Concepción; Pérez García, Lucas; González, M. TeresaElectrodes with nanostructured surface have emerged as promising low-impedance neural interfaces that can avoid the charge‐injection restrictions typically associated to microelectrodes. In this work, we propose a novel approximation, based on a two-step template assisted electrodeposition technique, to obtain flexible nanostructured electrodes coated with core–shell Ni–Au vertical nanowires. These nanowires benefit from biocompatibility of the Au shell exposed to the environment and the mechanical properties of Ni that allow for nanowires longer and more homogeneous in length than their only-Au counterparts. The nanostructured electrodes show impedance values, measured by electrochemical impedance spectroscopy (EIS), at least 9 times lower than those of flat reference electrodes. This ratio is in good accordance with the increased effective surface area determined both from SEM images and cyclic voltammetry measurements, evidencing that only Au is exposed to the medium. The observed EIS profile evolution of Ni–Au electrodes over 7 days were very close to those of Au electrodes and differently from Ni ones. Finally, the morphology, viability and neuronal differentiation of rat embryonic cortical cells cultured on Ni–Au NW electrodes were found to besimilar to those on control (glass) substrates and Au NW electrodes, accompanied by a lower glial cell differentiation. This positive in-vitro neural cell behavior encourages further investigation to explore the tissue responses that the implantation of these nanostructured electrodes might elicit in healthy (damaged) neural tissues in vivo, with special emphasis on eventual tissue encapsulation.Item Impact of magnetite nanowires on in vitro hippocampal neural networks(Biomolecules, 2023) Cortés Llanos, Belén; Rauti, Rossana; Ayuso Sacido, Ángel; Pérez García, Lucas; Ballerini, LauraNanomaterials design, synthesis, and characterization are ever-expanding approaches toward developing biodevices or neural interfaces to treat neurological diseases. The ability of nanomaterials features to tune neuronal networks’ morphology or functionality is still under study. In this work, we unveil how interfacing mammalian brain cultured neurons and iron oxide nanowires’ (NWs) orientation affect neuronal and glial densities and network activity. Iron oxide NWs were synthesized by electrodeposition, fixing the diameter to 100 nm and the length to 1 µm. Scanning electron microscopy, Raman, and contact angle measurements were performed to characterize the NWs’ morphology, chemical composition, and hydrophilicity. Hippocampal cultures were seeded on NWs devices, and after 14 days, the cell morphology was studied by immunocytochemistry and confocal microscopy. Live calcium imaging was performed to study neuronal activity. Using random nanowires (R-NWs), higher neuronal and glial cell densities were obtained compared with the control and vertical nanowires (V-NWs), while using V-NWs, more stellate glial cells were found. R-NWs produced a reduction in neuronal activity, while V-NWs increased the neuronal network activity, possibly due to a higher neuronal maturity and a lower number of GABAergic neurons, respectively. These results highlight the potential of NWs manipulations to design ad hoc regenerative interfaces.