Person: Pastrana Robles, Cristina
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First Name
Cristina
Last Name
Pastrana Robles
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
Identifiers
6 results
Search Results
Now showing 1 - 6 of 6
Item Dual-Mode Gold Nanoparticle-Based Method for Early Detection of Acanthamoeba(International Journal of Molecular Sciences, 2022) Pastrana Robles, Cristina; Guerreiro, Joana Rafaela L.; Elumalai, Monisha; Carpena Torres, Carlos; Crooke Álvarez, Almudena; Carracedo Rodríguez, Juan Gonzalo; Prado, Marta; Huete Toral, FernandoAcanthamoeba keratitis is an aggressive and rapidly progressing ocular pathology whose main risk factor is the use of contact lenses. An early and differential diagnosis is considered the main factor to prevent the progression and improve the prognosis of the pathology. However, current diagnosis techniques require time, complex and costly materials making an early diagnosis challenging. Thus, there is a need for fast, accessible, and accurate methods for Acanthamoeba detection by practitioners for timely and suitable treatment and even for contact lens user as preventive diagnosis. Here, we developed a dual-mode colorimetric-based method for fast, visual, and accurate detection of Acanthamoeba using gold nanoparticles (AuNPs). For this strategy, AuNPs were functionalized with thiolated probes and the presence of target Acanthamoeba genomic sequences, produce a colorimetric change from red to purple. This approach allows the detection of 0.02 and 0.009 μM of the unamplified Acanthamoeba genome by the naked eye in less than 20 min and by color analysis using a smartphone. Additionally, real samples were successfully analyzed showing the potential of the technology considering the lack of point-of-care tools that are mostly needed.Item Contact Lenses as Drug Delivery System for Glaucoma: A Review(Applied sciences, 2020) Peral Cerda, María Asunción; Martínez Águila, Alejandro; Pastrana Robles, Cristina; Huete Toral, Fernando; Carpena Torres, Carlos; Carracedo Rodríguez, Juan GonzaloGlaucoma is an optical neuropathy associated to a progressive degeneration of retinal ganglion cells with visual field loss and is the main cause of irreversible blindness in the world. The treatment has the aim to reduce intraocular pressure. The first therapy option is to instill drugs on the ocular surface. The main limitation of this is the reduced time of the drug staying on the cornea. This means that high doses are required to ensure its therapeutic effect. A drug-loaded contact lens can diffuse into the post lens tear film in a constant and prolonged flow, resulting in an increased retention of the drug on the surface of the cornea for up to 30 min and thus providing a higher drug bioavailability, increasing the therapeutic efficacy, reducing the amount of administered drug, and thereby provoking fewer adverse events. Several different systems of drug delivery have been studied in recent decades; ranging from more simple methods of impregnating the lenses, such as soaking, to more complex ones, such as molecular imprinting have been proposed. Moreover, different drugs, from those already commercially available to new substances such as melatonin have been studied to improve the glaucoma treatment efficacy. This review describes the role of contact lenses as an innovative drug delivery system to treat glaucoma.Item Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride(Journal of Ophthalmology, 2020) Carpena Torres, Carlos; Pintor Just, Jesús Jerónimo; Pérez de Lara, María Jesús; Huete Toral, Fernando; Crooke Álvarez, Almudena; Pastrana Robles, Cristina; Carracedo Rodríguez, Juan GonzaloPurpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia (P < 0.001), corneal staining (P < 0.001), conjunctival hyperemia (P < 0.001), and levels of IL-6 mRNA (P = 0.005) compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia (P < 0.001), tear breakup time (P = 0.007), tear osmolarity (P < 0.001), density of goblet cells (P < 0.001), and height of mucin cloud (P < 0.001). Conclusions. )e topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days).Item PhDAY 2020 -FOO (Facultad de Óptica y Optometría)(2020) Pintor Just, Jesús Jerónimo; Carpena Torres, Carlos; Peral Cerda, María Asunción; Pérez de Lara, María Jesús; Toral, Fernando; Crooke Álvarez, Almudena; Pastrana Robles, Cristina; Carracedo Rodríguez, Juan Gonzalo; Cayuela López, Ana; Sorzano Sánchez, Óscar; Charbel, Carla; Garzón Jiménez, Nuria; Carballo Álvarez, Jesús; Diz Arias, Elena; Fernández Jiménez, Elena; Lledó Mayans, Victoria Eugenia; Gómez Pedrero, José Antonio; Durán Prieto, Elena; López Alonso, José Manuel; Fernández Torres, Miguel Ángel; Guzmán Aránguez, Ana Isabel; Gómez Manzanares, Ángela; Vázquez Molini, Daniel; Martínez Antón, Juan Carlos; Bernárdez Vilaboa, Ricardo; Mayorga Pinilla, Santiago; Álvarez Fernández-Balbuena, Antonio; Benítez, AntoJ.; Igalla El-Youssfi, Asmae; León Álvarez, Alejandro; Palomo Álvarez, Catalina; Awad Alkozi, Hanan; Sánchez Naves, Juan; Martínez Alberquilla, Irene; García Montero, María; Ruiz Alcocer, Javier; Madrid Costa, David; Martínez Florentín, Gema; Papas, Eric B.; Medrano Muñoz, Sandra Milena; Molina, Nancy; Jurado, Sandra; Oliveiros López, Juan; Platero Alvarado, Nadiuska Cristine; Garrido Mercado, Rafaela; Pérez Garmendia, Carlos; Antona Peñalba, Beatriz; Barrio De Santos, Ana Rosa; González Pérez, Mariano; Pérez Garmendia, Carlos; Serramito Blanco, María; Privado Aroco, Ana; Almalki, Wael; Bodas Romero, Julia; Ouzzani, Mohamed; Paune, Jaume; Calderón García, Raquel; Pitarch Velasco, Aída; Cebrián, José Luis; Sánchez Pérez, María Isabel; García Rojo, Marta María; Bonnin Arias, Cristina Natalia; Sánchez Ramos, Celia; Gutiérrez Jorrín, Sara Carmen; Rodríguez Alonso, Xabier; Laucirica Sáenz, Gorka; Arranz Márquez, Esther; Alonso Castellanos, Miriam; Teus Guezala, Miguel Ángel; Hernández Verdejo, José Luis; Mármol Errasti, Esther; Martín García, Beatriz; Arriola Villalobos, Pedro; Gómez De Liaño Sánchez, María Rosario; Mínguez Caro, N; Orduña Azcona, Javier; Navarro Gil, Francisco Javier; Huete Toral, Fernando; Rodríguez Pomar, Candela; Martínez Águila, Alejandro; Martín Gil, Alba; Tomé de la Torre, Miguel ÁngelPor cuarto año consecutivo los doctorandos de la Facultad de Óptica y Optometría de la Universidad Complutense de Madrid cuentan con un congreso propio organizado por y para ellos, el 4º PhDAY- FOO. Se trata de un congreso gratuito abierto en la que estos jóvenes científicos podrán presentar sus investigaciones al resto de sus compañeros predoctorales y a toda la comunidad universitaria que quiera disfrutar de este evento. Apunta en tu agenda: el 15 de octubre de 2020. En esta ocasión será un Congreso On-line para evitar que la incertidumbre asociada a la pandemia Covid-19 pudiera condicionar su celebración.Item From oxidative stress to inflammation in the posterior ocular diseases: Diagnosis and treatment(Pharmaceutics, 2021) Dammak, Azza; Huete Toral, Fernando; Carpena Torres, Carlos; Martín Gil, Alba; Pastrana Robles, Cristina; Carracedo Rodríguez, Juan GonzaloMost irreversible blindness observed with glaucoma and retina-related ocular diseases, including age-related macular degeneration and diabetic retinopathy, have their origin in the posterior segment of the eye, making their physiopathology both complex and interconnected. In addition to the age factor, these diseases share the same mechanism disorder based essentially on oxidative stress. In this context, the imbalance between the production of reactive oxygen species (ROS) mainly by mitochondria and their elimination by protective mechanisms leads to chronic inflammation. Oxidative stress and inflammation share a close pathophysiological process, appearing simultaneously and suggesting a relationship between both mechanisms. The biochemical end point of these two biological alarming systems is the release of different biomarkers that can be used in the diagnosis. Furthermore, oxidative stress, initiating in the vulnerable tissue of the posterior segment, is closely related to mitochondrial dysfunction, apoptosis, autophagy dysfunction, and inflammation, which are involved in each disease progression. In this review, we have analyzed (1) the oxidative stress and inflammatory processes in the back of the eye, (2) the importance of biomarkers, detected in systemic or ocular fluids, for the diagnosis of eye diseases based on recent studies, and (3) the treatment of posterior ocular diseases, based on long-term clinical studies.Item Oxidative Stress in the Anterior Ocular Diseases: Diagnostic and Treatment(Biomedicines, 2023) Dammak, Azza; Pastrana Robles, Cristina; Martín Gil, Alba; Carpena Torres, Carlos; Peral Cerda, María Asunción; Simovart, Mirjam; Alarma-Estrany, Pilar; Huete Toral, Fernando; Carracedo Rodríguez, Juan GonzaloThe eye is a metabolically active structure, constantly exposed to solar radiations making its structure vulnerable to the high burden of reactive oxygen species (ROS), presenting many molecular interactions. The biomolecular cascade modification is caused especially in diseases of the ocular surface, cornea, conjunctiva, uvea, and lens. In fact, the injury in the anterior segment of the eye takes its origin from the perturbation of the pro-oxidant/antioxidant balance and leads to increased oxidative damage, especially when the first line of antioxidant defence weakens with age. Furthermore, oxidative stress is related to mitochondrial dysfunction, DNA damage, lipid peroxidation, protein modification, apoptosis, and inflammation, which are involved in anterior ocular disease progression such as dry eye, keratoconus, uveitis, and cataract. The different pathologies are interconnected through various mechanisms such as inflammation, oxidative stress making the diagnostics more relevant in early stages. The end point of the molecular pathway is the release of different antioxidant biomarkers offering the potential of predictive diagnostics of the pathology. In this review, we have analysed the oxidative stress and inflammatory processes in the front of the eye to provide a better understanding of the pathomechanism, the importance of biomarkers for the diagnosis of eye diseases, and the recent treatment of anterior ocular diseases.