Person: Ambrosio Elejalde, Nagore
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First Name
Nagore
Last Name
Ambrosio Elejalde
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Odontología
Department
Especialidades Clínicas Odontológicas
Area
Estomatología
Identifiers
5 results
Search Results
Now showing 1 - 5 of 5
Item Detection and quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Streptococcus oralis in blood samples with different microbiological identification methods: An in vitro study(Archives of Oral Biology, 2017) Marín Cuenda, María José; Ambrosio Elejalde, Nagore; Virto Ruiz, Leire; Diz, Pedro; Álvarez, Maximiliano; Herrera, David; Sanz Alonso, Mariano; Figuero Ruiz, ElenaBackground: Culture-based methods (culture broth bottles or lysis methods) have been the standard for detecting bacteremia. More recently, quantitative polymerase chain reaction (qPCR) was proposed as a more sensitive and specific test although none of them has been validated for the identification of periodontal pathogens (fastidious growing bacteria) in blood samples. Objective: To compare the ability to detect and quantify Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Streptococcus oralis (alone or in combination) in blood samples with three culture techniques [direct anaerobic culturing (DAC), haemo-culture (BACTEC), and lysis-centrifugation (LC)] and a non-culture dependent approach (qPCR) in an in vitro study. Material and methods: Blood samples from 12 periodontally healthy volunteers were contaminated with three concentrations [104,102 and 101 colony forming units (CFU)/mL] of A. actinomycetemcomitans, P. gingivalis and S. oralis, alone or in combination. Samples were analysed by DAC, BACTEC, LC and qPCR. Sensitivity, specificity, predictive values, kappa index and Lińs correlation coefficients were calculated. Results: DAC, LC and qPCR were able to detect the three target species at all concentrations. An excellent concordance (correlation coefficient r: 0.92-1) was observed between DAC and the reference standard (sensitivity raging 93.33-100% and specificity 88.89-100%) values. BACTEC was not able to identify P. gingivalis in any of the performed experiments. qPCR provided false negative results for S.oralis. Conclusions: DAC showed the best results for the proper identification and quantification of A. actinomycetemcomitans, P. gingivalis and S. oralis, alone or in combination, in blood samples.Item Periodontal diseases and depression: A pre‐clinical in vivo study(Journal of Clinical Periodontology, 2021) Martínez, María; Martín‐Hernández, David; Virto Ruiz, Leire; Mac-Dowell Mata, Karina Soledad; Leza Cerro, Juan Carlos; García Bueno, Borja; Figuero Ruiz, Elena; Ambrosio Elejalde, Nagore; Herrera González, David; Montero Solís, Eduardo; González Bris, Álvaro; Marín Cuenda, María José; Sanz Martín, MarianoAim: To analyse, through a pre-clinical in vivo model, the possible mechanisms linking depression and periodontitis at behavioural, microbiological and molecular levels. Materials and methods: Periodontitis (P) was induced in Wistar:Han rats (oral gavages with Porphyromonas gingivalis and Fusobacterium nucleatum) during 12 weeks, followed by a 3-week period of Chronic Mild Stress (CMS) induction. Four groups (n = 12 rats/group) were obtained: periodontitis and CMS (P+CMS+); periodontitis without CMS; CMS without periodontitis; and control. Periodontal clinical variables, alveolar bone levels (ABL), depressive-like behaviour, microbial counts and expression of inflammatory mediators in plasma and brain frontal cortex (FC), were measured. ANOVA tests were applied. Results: The highest values for ABL occurred in the P+CMS+ group, which also presented the highest expression of pro-inflammatory mediators (TNF-α, IL-1β and NF-kB) in frontal cortex, related to the lipoprotein APOA1-mediated transport of bacterial lipopolysaccharide to the brain and the detection of F. nucleatum in the brain parenchyma. A dysregulation of the hypothalamic-pituitary-adrenal stress axis, reflected by the increase in plasma corticosterone and glucocorticoid receptor levels in FC, was also found in this group. Conclusions: Neuroinflammation induced by F. nucleatum (through a leaky mouth) might act as the linking mechanism between periodontal diseases and depression.Item Detection and quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Streptococcus oralis in blood samples with different microbiological identification methods(Archives of Oral Biology, 2016) Herrera, David; Sanz, Mariano; Figuero, Elena; Marín Cuenda, María José; Ambrosio Elejalde, Nagore; Virto Ruiz, LeireBackground: Culture-based methods (culture broth bottles or lysis methods) have been the standard for detecting bacteremia. More recently, quantitative polymerase chain reaction (qPCR) was proposed as a more sensitive and specific test although none of them has been validated for the identification of periodontal pathogens (fastidious growing bacteria) in blood samples. Objective: To compare the ability to detect and quantify Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Streptococcus oralis (alone or in combination) in blood samples with three culture techniques [direct anaerobic culturing (DAC), haemo-culture (BACTEC), and lysis-centrifugation (LC)] and a non-culture dependent approach (qPCR) in an in vitro study. Material and methods: Blood samples from 12 periodontally healthy volunteers were contaminated with three concentrations [104,102 and 101 colony forming units (CFU)/mL] of A. actinomycetemcomitans, P. gingivalis and S. oralis, alone or in combination. Samples were analysed by DAC, BACTEC, LC and qPCR. Sensitivity, specificity, predictive values, kappa index and Lińs correlation coefficients were calculated. Results: DAC, LC and qPCR were able to detect the three target species at all concentrations. An excellent concordance (correlation coefficient r: 0.92-1) was observed between DAC and the reference standard (sensitivity raging 93.33-100% and specificity 88.89-100%) values. BACTEC was not able to identify P. gingivalis in any of the performed experiments. qPCR provided false negative results for S.oralis. Conclusions: DAC showed the best results for the proper identification and quantification of A. actinomycetemcomitans, P. gingivalis and S. oralis, alone or in combination, in blood samples.Item Bacteriemias de origen periodontal: validación de técnicas microbiológicas(2020) Ambrosio Elejalde, Nagore; Figuero Ruiz, Elena; Herrera González, DavidANTECEDENTES: El paso de bacterias a sangre (bacteriemias) se ha postulado como uno de los posibles mecanismos responsables de la relación entre la periodontitis y ciertas enfermedades sistémicas. Pocos son los estudios que buscan bacteriemias de origen periodontal que, a su vez, analicen y correlacionen los datos obtenidos en las muestras de sangre con los obtenidos en muestras subgingivales. Por otro lado, se ha demostrado que la acumulación del biofilm y la inflamación gingival aumentan la prevalencia de bacteriemias tras manipulaciones bucodentales profesionales y no profesionales, lo que podría justificar el análisis de esas bacterias a nivel subgingival en estos pacientes. OBJETIVOS: Validar y comparar los resultados obtenidos con diferentes técnicas microbiológicas para la detección de patógenos clave en muestras de sangre y en muestras subgingivales para el estudio de bacteriemias de origen periodontal. CONCLUSIONES: Los resultados de esta serie de estudios demuestran que la detección de patógenos periodontales, tanto en muestras de sangre como en muestras subgingivales, requiere de procesamiento y técnicas validadas para su identificación y cuantificación. Parece que las técnicas utilizadas en microbiología periodontal, tales como el CAD o la qPCR son las técnicas de elección en aquellos estudios en los que se quiera evaluar el paso de patógenos periodontales al torrente sanguíneo...Item Detección y cuantificación de bacterias asociadas a enfermedades periodontales en bacteriemias relacionadas con manipulaciones bucales no profesionales. Estudio piloto(2013) Ambrosio Elejalde, Nagore; Figuero Ruiz, Elena