Person:
Manzaneda González, Vanesa

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First Name
Vanesa
Last Name
Manzaneda González
URI
https://hdl.handle.net/20.500.14352/85508
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Física
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2019 - 201912020 - 20221
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Author: Almendro Vedia, Víctor Galileo ×

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    pH-triggered endosomal escape of pore-forming Listeriolysin O toxin-coated gold nanoparticles
    (Journal of Nanobiotechnology, 2019) Plaza-GA, Ismael; Manzaneda González, Vanesa; Kisovec, Matic; Almendro Vedia, Víctor Galileo; Muñoz Úbeda, Mónica; Anderluh, Gregor; Guerrero Martínez, Andrés; Natale, Paolo; López-Montero, Iván
    Background: A major bottleneck in drug delivery is the breakdown and degradation of the delivery system through the endosomal/lysosomal network of the host cell, hampering the correct delivery of the drug of interest. In nature, the bacterial pathogen Listeria monocytogenes has developed a strategy to secrete Listeriolysin O (LLO) toxin as a tool to escape the eukaryotic lysosomal system upon infection, allowing it to grow and proliferate unharmed inside the host cell. Results: As a “proof of concept”, we present here the use of purifed His-LLO H311A mutant protein and its conjuga tion on the surface of gold nanoparticles to promote the lysosomal escape of 40 nm-sized nanoparticles in mouse embryonic fbroblasts. Surface immobilization of LLO was achieved after specifc functionalization of the nanoparti cles with nitrile acetic acid, enabling the specifc binding of histidine-tagged proteins. Conclusions: Endosomal acidifcation leads to release of the LLO protein from the nanoparticle surface and its self-assembly into a 300 Å pore that perforates the endosomal/lysosomal membrane, enabling the escape of nanoparticles.
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    Tunable gold nanorod/NAO conjugates for selective drug delivery in mitochondria-targeted cancer therapy
    (Nanoscale, 2022) González-Rubio, Sergio; Salgado, Cástor; Manzaneda González, Vanesa; Muñoz Úbeda, Mónica; Ahijado Guzmán, Rubén; Natale, Paolo; Almendro Vedia, Víctor Galileo; Junquera González, María Elena; Osío Barcina, José De Jesús; Ferrer, Irene; Guerrero Martínez, Andrés; Paz-Ares Rodríguez, Luis Gonzaga; López Montero, Iván