Person:
Guerrero Martínez, Andrés

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First Name
Andrés
Last Name
Guerrero Martínez
URI
https://hdl.handle.net/20.500.14352/76239
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Física
Area
Química Física
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2022 - 20233
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Author: Manzaneda González, Vanesa × Subject: 544 ×

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Now showing 1 - 3 of 3
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    Effectiveness of Gold Nanorods of Different Sizes in Photothermal Therapy to Eliminate Melanoma and Glioblastoma Cells
    (International Journal of Molecular Sciences, 2023) Domingo-Diez, Javier; Souiade, Lilia; Manzaneda González, Vanesa; Sánchez-Díez, Marta; Megias, Diego; Guerrero Martínez, Andrés; Ramírez-Castillejo, Carmen; Serrano-Olmedo, Javier; Ramos-Gómez, Milagros
    Gold nanorods are the most commonly used nanoparticles in photothermal therapy for cancer treatment due to their high efficiency in converting light into heat. This study aimed to investigate the efficacy of gold nanorods of different sizes (large and small) in eliminating two types of cancer cell: melanoma and glioblastoma cells. After establishing the optimal concentration of nanoparticles and determining the appropriate time and power of laser irradiation, photothermal therapy was applied to melanoma and glioblastoma cells, resulting in the highly efficient elimination of both cell types. The efficiency of the PTT was evaluated using several methods, including biochemical analysis, fluorescence microscopy, and flow cytometry. The dehydrogenase activity, as well as calcein-propidium iodide and Annexin V staining, were employed to determine the cell viability and the type of cell death triggered by the PTT. The melanoma cells exhibited greater resistance to photothermal therapy, but this resistance was overcome by irradiating cells at physiological temperatures. Our findings revealed that the predominant cell-death pathway activated by the photothermal therapy mediated by gold nanorods was apoptosis. This is advantageous as the presence of apoptotic cells can stimulate antitumoral immunity in vivo. Considering the high efficacy of these gold nanorods in photothermal therapy, large nanoparticles could be useful for biofunctionalization purposes. Large nanorods offer a greater surface area for attaching biomolecules, thereby promoting high sensitivity and specificity in recognizing target cancer cells. Additionally, large nanoparticles could also be beneficial for theranostic applications, involving both therapy and diagnosis, due to their superior detection sensitivity.
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    Tunable gold nanorod/NAO conjugates for selective drug delivery in mitochondria-targeted cancer therapy
    (Nanoscale, 2022) González-Rubio, Sergio; Salgado, Cástor; Manzaneda González, Vanesa; Muñoz Úbeda, Mónica; Ahijado Guzmán, Rubén; Natale, Paolo; Almendro Vedia, Víctor Galileo; Junquera González, María Elena; Osío Barcina, José De Jesús; Ferrer, Irene; Guerrero Martínez, Andrés; Paz-Ares Rodríguez, Luis Gonzaga; López Montero, Iván
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    From multi- to single-hollow trimetallic nanocrystals by ultrafast heating
    (Chemistry of Materials, 2023) Manzaneda González, Vanesa; Jenkinson, Kellie; Peña-Rodríguez, Ovidio; Borrell Grueiro, Olivia; Triviño-Sánchez, Sergio; Bañares Morcillo, Luis; Junquera González, María Elena; Espinosa, Ana; González-Rubio, Guillermo; Bals, Sara; Guerrero Martínez, Andrés
    Metal nanocrystals (NCs) display unique physicochemical features that are highly dependent on the nanoparticle dimensions, anisotropy, structure, and composition. The development of synthesis methodologies that allow us to tune such parameters finely emerges crucial for the application of metal NCs in catalysis, optical materials, or biomedicine. Here, we describe a synthetic methodology to fabricate hollow multimetallic heterostructures using a combination of seed-mediated growth routes and femtosecond pulsed laser irradiation. The envisaged methodology relies on the co-reduction of Ag and Pd ions on gold nanorods (Au NRs) to form Au@PdAg core–shell nanostructures containing small cavities at the Au-PdAg interface. The excitation of Au@PdAg NRs with low fluence femtosecond pulses was employed to induce the coalescence and growth of large cavities, forming multihollow anisotropic Au@PdAg nanostructures. Moreover, single-hollow alloy AuPdAg could be achieved in high yield by increasing the irradiation energy. Advanced electron microscopy techniques, energy-dispersive X-ray spectroscopy (EDX) tomography, X-ray absorption near edge structure (XANES) and FDTD (finite differences in the time domain) simulationsallowed us to characterize the morphology, structure, and elemental distribution of the irradiated NCs in detail. The ability of the reported synthesis route to fabricate multimetallic NCs with unprecedented hollow nanostructures brings attractive prospects for the fabrication of tailored high-entropy alloy nanoparticles.