Person:
Zabala Gutiérrez, Irene

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First Name
Irene
Last Name
Zabala Gutiérrez
URI
https://hdl.handle.net/20.500.14352/86286
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Química en Ciencias Farmacéuticas
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2020 - 20212
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Author: Fernández Monsalve, Nuria × Subject: 535.37 ×

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Now showing 1 - 2 of 2
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    Reaching Deeper: Absolute In Vivo Thermal Reading of Liver by Combining Superbright Ag2S Nanothermometers and In Silico Simulations
    (Advanced Science, 2021) Lifante Cañavate, José; Shen, Yingli; Zabala Gutiérrez, Irene; Rubia Rodríguez, Irene; Ortega, Daniel; Fernández Monsalve, Nuria; Melle Hernández, Sonia; Granado García, Miriam; Rubio Retama, Jorge; Jaque García, Daniel; Ximendes, Erving Clayton
    Luminescent nano‐thermometry is a fast‐developing technique with great potential for in vivo sensing, diagnosis, and therapy. Unfortunately, it presents serious limitations. The luminescence generated by nanothermometers, from which thermal readout is obtained, is strongly distorted by the attenuation induced by tissues. Such distortions lead to low signal levels and entangle absolute and reliable thermal monitoring of internal organs. Overcoming both limitations requires the use of high‐brightness luminescent nanothermometers and adopting more complex approaches for temperature estimation. In this work, it is demonstrated how superbright Ag2S nanothermometers can provide in vivo, reliable, and absolute thermal reading of the liver during laser‐induced hyperthermia. For that, a new procedure is designed in which thermal readout is obtained from the combination of in vivo transient thermometry measurements and in silico simulations. The synergy between in vivo and in silico measurements has made it possible to assess relevant numbers such as the efficiency of hyperthermia processes, the total heat energy deposited in the liver, and the relative contribution of Ag2S nanoparticles to liver heating. This work provides a new way for absolute thermal sensing of internal organs with potential application not only to hyperthermia processes but also to advanced diagnosis and therapy.
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    Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging
    (Nature communications, 2020) Santos, Harrison D. A.; Zabala Gutiérrez, Irene; Shen, Yingli; Lifante, José; Ximendes, Erving; Laurenti, Marco; Méndez González, Diego; Melle Hernández, Sonia; Gómez Calderón, Óscar; López Cabarcos, Enrique; Fernández Monsalve, Nuria; Chavez Coria, Irene; Lucena Agell, Daniel; Monge, Luis; Mackenzie, Mark D.; Marqués Hueso, José; Jones, Callum M. S.; Jacinto, Carlos; Rosal, Blanca, del; Kar, Ajoy K.; Rubio Retama, Jorge; Jaque García, Daniel
    Optical probes operating in the second near-infrared window (NIR-II, 1,000-1,700 nm), where tissues are highly transparent, have expanded the applicability of fluorescence in the biomedical field. NIR-II fluorescence enables deep-tissue imaging with micrometric resolution in animal models, but is limited by the low brightness of NIR-II probes, which prevents imaging at low excitation intensities and fluorophore concentrations. Here, we present a new generation of probes (Ag2S superdots) derived from chemically synthesized Ag2S dots, on which a protective shell is grown by femtosecond laser irradiation. This shell reduces the structural defects, causing an 80-fold enhancement of the quantum yield. PEGylated Ag2S superdots enable deep-tissue in vivo imaging at low excitation intensities (<10 mW cm−2) and doses (<0.5 mg kg−1), emerging as unrivaled contrast agents for NIR-II preclinical bioimaging. These results establish an approach for developing superbright NIR-II contrast agents based on the synergy between chemical synthesis and ultrafast laser processing.