Person:
Cañadas Benito, Olga

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First Name
Olga
Last Name
Cañadas Benito
URI
https://hdl.handle.net/20.500.14352/74363
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Department
Bioquímica y Biología Molecular
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Bioquímica y Biología Molecular
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Author: Casals Carro, María Cristina × Author: Cañadas Benito, Olga × End date: 2012 × Start date: 2010 × Item Type: Publication × Has files: true × Subject: 577.1 ×

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    Differential scanning calorimetry of protein-lipid interactions
    (Lipid-protein interactions: Methods and Protocols, 2012) Cañadas Benito, Olga; Casals Carro, María Cristina; Kleinchmidt, Jorg
    Differential scanning calorimetry (DSC) is a highly sensitive non-perturbing technique for measuring the thermodynamic properties of thermally induced transitions. This technique is particularly useful for the characterization of lipid/protein interactions. This chapter presents an introduction to DSC instrumentation, basic theory, and methods and describes DSC applications for characterizing protein effects on model lipid membranes. Examples of the use of DSC for the evaluation of protein effects on modulation of membrane domains and membrane stability are given.