Person: Cañadas Benito, Olga
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First Name
Olga
Last Name
Cañadas Benito
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Department
Bioquímica y Biología Molecular
Area
Bioquímica y Biología Molecular
Identifiers
5 results
Search Results
Now showing 1 - 5 of 5
Item Insights into the mechanisms of interaction between inhalable lipid-polymer hybrid nanoparticles and pulmonary surfactant(Journal of Colloid and Interface Science, 2022) Xu, You; Parra-Ortiz, Elisa; Wan, Feng; Cañadas Benito, Olga; García Álvarez, María Begoña; Thakur, Aneesh; Franzyk, Henrik; Pérez Gil, Jesús; Malmsten, Martin; Foged, CamillaPulmonary delivery of small interfering RNA (siRNA) using nanoparticle-based delivery systems is promising for local treatment of respiratory diseases. We designed dry powder inhaler formulations of siRNA-loaded lipid-polymer hybrid nanoparticles (LPNs) with aerosolization properties optimized for inhalation therapy. Interactions between LPNs and pulmonary surfactant (PS) determine the fate of inhaled LPNs, but interaction mechanisms are unknown. Here we used surface-sensitive techniques to study how physicochemical properties and pathological microenvironments influence interactions between siRNA-loaded LPNs and supported PS layers. PS was deposited on SiO2 surfaces as single bilayer or multilayers and characterized using quartz crystal microbalance with dissipation monitoring and Fourier-transform infrared spectroscopy with attenuated total reflection. Immobilization of PS as multilayers, resembling the structural PS organization in the alveolar subphase, effectively reduced the relative importance of interactions between PS and the underlying surface. However, the binding affinity between PS and LPNs was identical in the two models. The physicochemical LPN properties influenced the translocation pathways and retention time of LPNs. Membrane fluidity and electrostatic interactions were decisive for the interaction strength between LPNs and PS. Experimental conditions reflecting pathological microenvironments promoted LPN deposition. Hence, these results shed new light on design criteria for LPN transport through the air–blood barrier.- Project number: 306
Item La comunidad del anillo IGGIA: construyendo redes de mentoría en Ingeniería Genética mediante gamificación, internacionalización y accesibilidad(2022) Sánchez Torralba, Antonio; Benítez Prian, Mario; Blázquez Ortiz, Cristina; Bruñén Alfaro, Francisco; Cañadas Benito, Olga; García de la Camacha Selgas, Nuria; García-Fojeda García-Valdecasas, Belén; González Miranda, David; Guevara Acosta, Govinda; López Conejo, María Teresa; Lorente Pérez, María del Mar; Mateo Mendoza, Jorge Mario; Nogués Vera, Laura; Raisman, Andrea; Ranz Valdecasa, María Regina; Ruiz Ortega, Marta; Sánchez-Escalonilla Relea, Jose Luis; Sánchez Velasco, Teresa; Toledo Marcos, Juan; Velasco Díez, Guillermo; Navarro Llorens, Juana María - Project number: 225
Item H5P-Pandemic: Motivando al alumnado mediante ejercicios interactivos ludificados en asignaturas de ingeniería genética(2023) Sánchez Torralba, Antonio; Lorente Pérez, Mª delMar; Blázquez Ortiz, Cristina; Velasco Díez, Guillermo; Ranz Valdecasas, Mª Regina; López Conejo, Mª Teresa; García-Fojeda García-Valdecasas, Mª Belén; Nogués Vera, Laura; Cañadas Benito, Olga; Guevara Acosta, Flor Govinda; Ruiz Ortega, Marta; Rayego Mateos, Sandra; Sánchez Velasco, Teresa; Mateo Mendoza, Jorge Mario; Navarro Llorens, Juana MªFundamentos de Ingeniería Genética y Genómica (Grado en Biología) se ludificó con éxito mediante un juego de pandemias. Se ha mejorado la participación y el autoaprendizaje del alumnado con ejercicios autoevaluables de tipo H5P y exportables a Moodle. Item Pulmonary surfactant inactivation by β-D-glucan and protective role of surfactant protein A(Colloids and Surfaces B: Biointerfaces, 2022) Cañadas Benito, Olga; Sáenz Martínez, Alejandra; Lorenzo Avilés, Alba de; Casals Carro, CristinaPulmonary fungal infections lead to damage of the endogenous lung surfactant system. However, the molecular mechanism underlying surfactant inhibition is unknown. β-D-glucan is the major component of pathogenic fungal cell walls and is also present in organic dust, which increases the risk of respiratory diseases. The objective of this study was to characterize the interaction of this D-glucopyranose polymer with pulmonary surfactant. Our results show that β-D-glucan induced a concentration-dependent inhibition of the surface adsorption, respreading, and surface tension-lowering activity of surfactant preparations containing surfactant proteins SP-B and SP-C. Our data support a new mechanism of surfactant inhibition that consists in the extraction of phospholipid molecules from surfactant membranes by β-D-glucan. As a result, surfactant membranes became more fluid, as demonstrated by fluorescence anisotropy, and showed decreased Tm and transition enthalpy. Surfactant preparations containing surfactant protein A (SP-A) were more resistant to β-D-glucan inhibition. SP-A bound to different β-D-glucans with high affinity (Kd = 1.5 ± 0.1 nM), preventing and reverting β-D-glucan inhibitory effects on surfactant interfacial adsorption and partially abrogating β-D-glucan inhibitory effects on surfactant’s reduction of surface tension. We conclude that β-D-glucan inhibits the biophysical function of surfactant preparations lacking SP-A by subtraction of phospholipids from surfactant bilayers and monolayers. The increased resistance of SP-A-containing surfactant preparations to β-D-glucan reinforces its use in surfactant replacement therapy.- Project number: PIMCD327/23-24
Item El poder de la nanotecnología lipídica: aplicaciones biotecnológicas de la asignatura Estructura de Membranas Biológicas(2024) Cañadas Benito, Olga; Ballesteros Aparicio, Sara; Cruz Rodríguez, Antonio; Espada Espinar, Adrián; Fuertes Vázquez, Lucía; García-Fojeda García-Valdecasas, María Belén; Gutiérrez Ramos, Irene