Person:
Pastor Vargas, Carlos

First Name

Carlos

Last Name

Pastor Vargas

URI

https://hdl.handle.net/20.500.14352/76123

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Ciencias Químicas

Department

Bioquímica y Biología Molecular

Area

Bioquímica y Biología Molecular

Identifiers

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Now showing 1 - 10 of 35

Identification of thaumatin‐like protein and aspartyl protease as new major allergens in lettuce (Lactuca sativa).
(Molecular nutrition and food research, 2013) Muñoz‐García, Esther; Luengo‐Sánchez, Olga; Haroun‐Díaz, Elisa; Sanz Maroto, Aroa; Palacín, Arancha; Díaz –Perales, Araceli; de las Heras Gozalo, Manuel; Labrador‐Horrillo, Moisés; Vivanco Martínez, Fernando; Cuesta‐Herranz, Javier; Pastor Vargas, Carlos
Scope: Today, about 2-8% of the population of Western countries exhibits some type of food allergy whose impact ranges from localized symptoms confined to the oral mucosa to severe anaphylactic reactions. Consumed worldwide, lettuce is a Compositae family vegetable that can elicit allergic reactions. To date, however, only one lipid transfer protein has been described in allergic reaction to lettuce. The aim of this study was to identify potential new allergens involved in lettuce allergy. Methods and results: Sera from 42 Spanish lettuce-allergic patients were obtained from patients recruited at the outpatient clinic. IgE-binding proteins were detected by SDS-PAGE and immunoblotting. Molecular characterization of IgE-binding bands was performed by MS. Thaumatin was purified using the Agilent 3100 OFFGEL system. The IgE-binding bands recognized in the sera of more than 50% of patients were identified as lipid transfer protein (9 kDa), a thaumatin-like protein (26 kDa), and an aspartyl protease (35 and 45 kDa). ELISA inhibition studies were performed to confirm the IgE reactivity of the purified allergen. Conclusion: Two new major lettuce allergens-a thaumatin-like protein and an aspartyl protease-have been identified and characterized. These allergens may be used to improve both diagnosis and treatment of lettuce-allergic patients.
Anaphylaxis Due to Oat Ingestion
(JIACI - Journal of Investigational Allergology and Clinical Immunology, 2016) Prados-Castaño M; Piñero-Saavedra M; Leguisamo-Milla S; Pastor Vargas, Carlos; Cuesta J; Bartolomé B
Allergy to goat’s and sheep’s milk in a population of cow’s milk–allergic children treated with oral immunotherapy*
(Pediatr Allergy Immunol, 2012) Rodríguez del Río, Pablo; Sánchez‐García, Silvia; Escudero, Carmelo; Pastor Vargas, Carlos; Sánchez Hernández, José J.; Pérez‐Rangel, Inmaculada; Ibáñez, María Dolores
Background: Cow's milk oral immunotherapy (CMOIT) is a recognized treatment for persistent cow's milk (CM) allergy. However, further data are necessary on tolerance to milk from other mammals. Objective: To describe the clinical and immunologic features of goat's and sheep's milk (GSM) allergy in patients who tolerated CM after CMOIT. Methods: Fifty-eight CM-allergic patients who successfully underwent CMOIT in our department were evaluated using skin prick test (SPT), specific immunoglobulin (Ig) E determination, enzyme-linked immunoassay (ELISA), and controlled oral challenge to assess allergy to GSM. Statistical analysis was carried out to identify markers of allergy to GSM. Results: Fifteen of 58 (25.9%) patients were allergic to either goat's or sheep's milk or to both, as confirmed by a controlled positive oral challenge. Forty-seven percent of all positive oral challenges were classified as anaphylactic reactions. Specific IgE to CM casein, goat's whole milk, and sheep's whole milk was 13.2, 18.0, and 21.4 kU(A)/l in the group of GSM-allergic patients and 6.6, 6.5, and 6.5 kU(A) /l in the GSM-non-allergic patients (p < 0.05). Decision-making cut-off points based on sIgE for diagnosing symptomatic GSM allergy could not be determined. ELISA inhibition assays showed limited cross-reactivity (up to 77.2%) between CM casein and GSM casein in the group of GSM-allergic patients in contrast with almost 100% in GSM-not-allergic patients. Conclusion: We found a high prevalence (26%) of allergy to GSM in our population of CM-allergic children treated with oral immunotherapy. Therefore, tolerance to GSM should be assessed in order to provide accurate nutritional advice and minimize life-threatening accidental intake. Specific IgE to CM casein, goat's and sheep's whole milk is a good marker of this allergy. Although CM oral immunotherapy is a specific treatment for CM allergy, it may not be effective against allergy to the milk of other mammals.
Occupational rhinitis due to inhalation of chicken meat protein.
(J Investig Allergol Clin Immunol, 2014) Lobera Labairu, T; González Mahave, I; Del Pozo Gil, M D; Venturini Díaz, M; Blasco Sarramián, A; Pastor Vargas, Carlos; Vivanco Martínez, Fernando; Bartolomé Zavala, Borja
Detection of major food allergens in amniotic fluid: initial allergenic encounter during pregnancy
(Pediatr Allergy Immunol, 2016) Pastor Vargas, Carlos; Aroa S. Maroto; Araceli Díaz‐Perales; Villalba Díaz, María Teresa; Vanesa Esteban; Marta Ruiz‐Ramos; Marta Rodriguez de Alba; Vivanco Martínez, Fernando; Javier Cuesta‐Herranz
Background: Ingestion of food allergens present in maternal milk during breastfeeding has been hypothesized as a gateway to sensitization to food; however, this process could develop during pregnancy, as the maternal-fetal interface develops a Th2- and Treg-mediated environment to protect the fetus. We hypothesized that in these surroundings, unborn children are exposed to food allergens contained in the mother's diet, possibly giving rise to first sensitization. Methods: The presence of allergens in utero was studied by analyzing amniotic fluid (AF) samples in two different stages of pregnancy: at 15-20 weeks and after delivery at term. An antibody microarray was developed to test for the most common food allergens. The array detects the presence of ten allergens from milk, fruit, egg, fish, nuts, and wheat. Results: AF from 20 pregnant women was collected: eight after delivery at term and 12 from women who underwent diagnostic amniocentesis between weeks 15 and 20 of gestation. The presence of allergens was detected in all samples. Samples from amniocentesis had a higher allergen concentration than samples after delivery at term. Conclusions: We demonstrated the presence of intact major food allergens in AF samples. This early contact could explain subsequent sensitization to foods never eaten before.
Allergy to Uncommon Pets: New Allergies but the Same Allergens
(Front. Immunol., 2013) Díaz-Perales, Araceli; González-de-Olano, David; Pérez-Gordo, Marina; Pastor Vargas, Carlos
The prevalence of exotic pet allergies has been increasing over the last decade. Years ago, the main allergy-causing domestic animals were dogs and cats, although nowadays there is an increasing number of allergic diseases related to insects, rodents, amphibians, fish, and birds, among others. The current socio-economic situation, in which more and more people have to live in small apartments, might be related to this tendency. The main allergic symptoms related to exotic pets are the same as those described for dog and cat allergy: respiratory symptoms. Animal allergens are therefore, important sensitizing agents and an important risk factor for asthma. There are three main protein families implicated in these allergies, which are the lipocalin superfamily, serum albumin family, and secretoglobin superfamily. Detailed knowledge of the characteristics of allergens is crucial to improvement treatment of uncommon-pet allergies.
Involvement of Can f 5 in a Case of Human Seminal Plasma Allergy
(International Archives of Allergy Immunology, 2012) Maria Basagaña; Borja Bartolome; Pastor Vargas, Carlos; Lars Mattsson; Jonas Lidholm; Moises Labrador-Horrillo
Background: The existence of IgE binding to dog dander extract without IgE antibodies against the described dog allergens (Can f 1, 2, 3 and 4) implies the presence of other dog allergens yet to be identified. Recently, an IgE-binding protein was isolated from dog urine and identified as prostatic kallikrein; it has been named Can f 5. Cross-reactivity between a dog dander allergen and human prostate-specific antigen (PSA) has been described. The aim of this study was to identify the dog dander allergen that presents cross-reactivity with PSA and demonstrate its clinical relevance in our patient with human seminal plasma allergy. Methods: SDS-PAGE immunoblotting and inhibition tests were performed. Mass spectrometry was carried out to identify the protein involved in the allergy reactions. Results: SDS-PAGE immunoblotting-inhibition with an IgE-binding protein from dog prostatic secretion showed total IgE binding inhibition to a 28-kDa IgE-reactive band identified as PSA. The electroeluted protein from dog prostatic secretion was identified by mass spectrometry as Can f 5. IgE immunoblotting of human seminal plasma incubated with the serum of the patient revealed two IgE-binding bands (28 and 32.7 kDa). Both SDS-PAGE immunoblotting inhibition assays, with human seminal plasma or purified PSA in solid phase, showed complete IgE binding inhibition when the serum of the anaphylactic patient was preincubated with dog dander extract or recombinant Can f 5. Conclusions: The dog dander allergen that shows cross-reactivity with human PSA has been characterized and turns out to be the recently described Can f 5. We demonstrated the clinical relevance of this cross-reactivity in a patient.
Angler fish is not an alternative in allergy to multiple fish species
(ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2014) Julián Azofra; Aroa Sanz Maroto; Vivanco Martínez, Fernando; Pastor Vargas, Carlos
Fish allergy is one of the most frequent food allergies. 1 Its prevalence is highest is those regions where consumption is greatest. Spain has one of the highest fish intakes per inhabitant and, as a result, fish allergy is a serious health problem.
First case of airborne-induced anaphylaxis triggered by fruit
(ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2015) Eva M. Macías; Omar Sierra-Salgado; Borja Bartolomé; Pastor Vargas, Carlos; Francisco J. Muñoz-Bellido; Ignacio Dávila
Identification of allergens in chicken meat allergy.
(J Investig Allergol Clin Immunol, 2011) Eloina González-Mancebo, Eloina; Pastor Vargas, Carlos; González-de-Olano, David; Gandolfo-Cano, Mar; Melendez, Amaya; Cuesta Herranz, Javier; Zapatero, A.; Bartolome, Borja