Person: Peral Cerda, María Asunción
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First Name
María Asunción
Last Name
Peral Cerda
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
Identifiers
10 results
Search Results
Now showing 1 - 10 of 10
Item Improving ocular surface comfort in contact lens wearers(Contact Lens and Anterior Eye, 2021) Fernández Jiménez, Elena; Diz Arias, Elena; Peral Cerda, María AsunciónPurpose Contact lens discomfort (CLD) is a major concern that can lead to the decreased or abandoned use of contact lenses. Contact lens users with dry eye disease are more likely to present with CLD. This study was conducted to evaluate the efficacy of a bioprotective preservative free, hypotonic, 0.15% hyaluronic acid (HA)-3% Trehalose artificial tear in managing dry eye symptoms in contact lens wearers. Methods A prospective, single-arm, observational pilot study to evaluate the effectiveness of treatment with HA-Trehalose artificial tears in contact lens wearers (N = 33) aged 18–45 years with symptoms of ocular discomfort. Participants used a preservative-free, hypotonic HA-Trehalose artificial tear (1 drop/4 times per day) for 84 days. Participants were assessed using Visual Analogue Scale (VAS) for dry eye symptoms (pain, photophobia, dry eye sensation, blurry vision, foreign body sensation, itching, tingling/burning, and sticky eye feeling), Ocular Surface Disease Index (OSDI), Contact Lens Dry Eye questionnaire (CLDEQ-8), Berkley Dry Eye Flow-Chart (DEFC) on Day 0 and Day 84 and tear break-up time (TBUT), ocular surface staining with fluorescein and lissamine green, tear meniscus evaluation, and visual acuity on Day 0, 35, and 84. Results All VAS symptoms (except tingling/burning and sticky eye feeling), OSDI, CLEDQ-8, and DEFC showed statistically significant (p < 0.05) improvement from baseline (Day 0) to Day 84. Similarly, corneal (fluorescein) and conjunctival (lissamine green) quality improved during the study (p < 0.05 at Day 84 versus baseline). Tear break-up time (TBUT), conjunctival (lissamine green) staining, and tear meniscus decreased but the changes were not statistically significant. Visual acuity did not change during the study. There were no ocular or systemic adverse events. Conclusions This study showed that the instillation of a preservative-free, hypotonic, HA-Trehalose artificial tear in contact lenses wearers with dry eye syndrome significantly improved symptoms and reduced associated signs such as corneal and conjunctival staining.Item Variation of Meibomian glands contrats throughout the day(2024) Peral Cerda, María Asunción; Fernandez Jimenez Elena; Diz Arias, Elena; Gómez Pedrero, José Antonio; Peral Cerda, María Asunción; Gómez Pedrero, José Antonio; Peral APurpose: To evaluate Meibomian glands (MG), specialized imaging techniques are used. The most commonly used technique for the observation of MG is non-contact meibography with infrared light. This technique allows to see specific details of the MG, provides quantitative measurements, in addition to structural appearance, shortening, distortions or atrophy. [1] Recent studies have shown that the analysis of MG contrasts can represent an advance in the diagnosis of related pathologies to MG, such as Meibomian gland dysfunction and Blepharitis. [2] The present study wants to observe the contrast variation throughout the day in the spaces between two adjacent MG, interglandular contrasts (CINTER) and within the same gland, intraglandular contrasts (CIN). The main objective of this study is to observe the variation of MG contrasts throughout the day and analyze its possible correlation with alterations related to these glands. Methods: A prospective, descriptive, pilot study was carried out on 8 participants, including controls, MG pathology participants, and contact lens users. A set of tests were performed, including symptomatology tests, clinical tests to recognize the ocular surface and an assessment of the MG. Images of the MG were taken throughout the day, with the Oculus Keratograph 5MTM. Meibography images were captured from 8 am to 8 pm in two hours slots. The eyelids were everted and the images were always captured by the same clinical examiner. The meibography images have been processed with the Matlab® program to analyze the CINTER and CIN contrast. [3] Results: Preliminary results show that there is an oscillatory and fluctuating trend in glandular contrasts throughout the day, following different patterns in each subject. Conclusions: As has been previously studied, MG contrasts are postulated as a possible biomarker of MG alterations. The study of the behavior of these contrasts throughout the day and in different groups of subjects may lead to advances in the diagnosis of MG alterations or pathologies such as Meibomian gland dysfunction or Blepharitis, and could also provide further insight on the meibum production cycle and MG physiology.Item Therapeutic potential of topical administration of siRNAs against HIF-1α for corneal neovascularization(Experimental Eye Research, 2022) Peral Cerda, María Asunción; Mateo Ibáñez, Jesús; Domínguez Godínez, Carmen Olalla; Carracedo Rodríguez, Juan Gonzalo; Gómez Pedrero, José Antonio; Crooke Álvarez, Almudena; Pintor Just, Jesús JerónimoGiven the implications of the problem of neovascularization on ocular health, as well as the growth in the number of cases, the purpose of the present study has been testing the efficacy of siRNAs (small interfering RNA) designed to silence Hypoxia Inducible Factor -1α (HIF-1α) and to demonstrate that their use stops neovascularization in a model of corneal burn. Corneal wounds in the limbic zone were made in the eyes of New Zealand white rabbits. Topical applications of siRNAs were done the next day to the wound for four consecutive days and eyes were examined with a slit lamp. Evaluation of neovascularization progress was done by analyzing images by ImageJTM and to determine the neovascular area in Matlab ® was used. At the same time, a rabbit corneal cell line was used for in vitro study of hypoxia exposure and Western blot analysis of the cell's extracts were done. Under normal cell culture oxygenation, the expression of HIF-1α was lower than that observed under hypoxic conditions. After 2 h of hypoxia, there was a significant increase in the HIF-1α expression, effect that was maintained up to 6 h. The increased in HIF-1α was mimicked by a cell permeable prolyl-4-hydroxylase inhibitor. Cobalt chloride showed no capacity to increase HIF-1α in vitro. The effect of three different siRNA on HIF-1α was tested after 4 h of hypoxia. siRNA#1 was able to silence 80% of HIF-1α expression, siRNA#2 and siRNA#3 reduce the expression in 45% and 40% respectively. In addition, the three siRNA were tested in a corneal model of neovascularization. scrambledsiRNA#2 was the most effective inhibitor of blood vessel production, followed by siRNA#3 and siRNA#1. Compared to the scrambled siRNA (100% of blood vessel generation), siRNA#2 blocked the presence of blood vessels by 83 ± 2%, siRNA#3 inhibited 45 ± 7% and siRNA#1 only inhibited 18 ± 5%. The necessary time to observe the 50% of effect showed values of NV50 of 10.2 ± 2.4 days for the scrambled siRNA, 9.1 ± 1.4 for siRNA#1, 6.5 ± 1.85 for siRNA#2 and 4.8 ± 1.8 days for siRNA#3. In conclusion, the topical application of siRNA towards HIF-1α seems to be an effective and reliable method to stop neovascularization.Item Contact Lenses as Drug Delivery System for Glaucoma: A Review(Applied sciences, 2020) Peral Cerda, María Asunción; Martínez Águila, Alejandro; Pastrana Robles, Cristina; Huete Toral, Fernando; Carpena Torres, Carlos; Carracedo Rodríguez, Juan GonzaloGlaucoma is an optical neuropathy associated to a progressive degeneration of retinal ganglion cells with visual field loss and is the main cause of irreversible blindness in the world. The treatment has the aim to reduce intraocular pressure. The first therapy option is to instill drugs on the ocular surface. The main limitation of this is the reduced time of the drug staying on the cornea. This means that high doses are required to ensure its therapeutic effect. A drug-loaded contact lens can diffuse into the post lens tear film in a constant and prolonged flow, resulting in an increased retention of the drug on the surface of the cornea for up to 30 min and thus providing a higher drug bioavailability, increasing the therapeutic efficacy, reducing the amount of administered drug, and thereby provoking fewer adverse events. Several different systems of drug delivery have been studied in recent decades; ranging from more simple methods of impregnating the lenses, such as soaking, to more complex ones, such as molecular imprinting have been proposed. Moreover, different drugs, from those already commercially available to new substances such as melatonin have been studied to improve the glaucoma treatment efficacy. This review describes the role of contact lenses as an innovative drug delivery system to treat glaucoma.Item PhDAY 2020 -FOO (Facultad de Óptica y Optometría)(2020) Pintor Just, Jesús Jerónimo; Carpena Torres, Carlos; Peral Cerda, María Asunción; Pérez de Lara, María Jesús; Toral, Fernando; Crooke Álvarez, Almudena; Pastrana Robles, Cristina; Carracedo Rodríguez, Juan Gonzalo; Cayuela López, Ana; Sorzano Sánchez, Óscar; Charbel, Carla; Garzón Jiménez, Nuria; Carballo Álvarez, Jesús; Diz Arias, Elena; Fernández Jiménez, Elena; Lledó Mayans, Victoria Eugenia; Gómez Pedrero, José Antonio; Durán Prieto, Elena; López Alonso, José Manuel; Fernández Torres, Miguel Ángel; Guzmán Aránguez, Ana Isabel; Gómez Manzanares, Ángela; Vázquez Molini, Daniel; Martínez Antón, Juan Carlos; Bernárdez Vilaboa, Ricardo; Mayorga Pinilla, Santiago; Álvarez Fernández-Balbuena, Antonio; Benítez, AntoJ.; Igalla El-Youssfi, Asmae; León Álvarez, Alejandro; Palomo Álvarez, Catalina; Awad Alkozi, Hanan; Sánchez Naves, Juan; Martínez Alberquilla, Irene; García Montero, María; Ruiz Alcocer, Javier; Madrid Costa, David; Martínez Florentín, Gema; Papas, Eric B.; Medrano Muñoz, Sandra Milena; Molina, Nancy; Jurado, Sandra; Oliveiros López, Juan; Platero Alvarado, Nadiuska Cristine; Garrido Mercado, Rafaela; Pérez Garmendia, Carlos; Antona Peñalba, Beatriz; Barrio De Santos, Ana Rosa; González Pérez, Mariano; Pérez Garmendia, Carlos; Serramito Blanco, María; Privado Aroco, Ana; Almalki, Wael; Bodas Romero, Julia; Ouzzani, Mohamed; Paune, Jaume; Calderón García, Raquel; Pitarch Velasco, Aída; Cebrián, José Luis; Sánchez Pérez, María Isabel; García Rojo, Marta María; Bonnin Arias, Cristina Natalia; Sánchez Ramos, Celia; Gutiérrez Jorrín, Sara Carmen; Rodríguez Alonso, Xabier; Laucirica Sáenz, Gorka; Arranz Márquez, Esther; Alonso Castellanos, Miriam; Teus Guezala, Miguel Ángel; Hernández Verdejo, José Luis; Mármol Errasti, Esther; Martín García, Beatriz; Arriola Villalobos, Pedro; Gómez De Liaño Sánchez, María Rosario; Mínguez Caro, N; Orduña Azcona, Javier; Navarro Gil, Francisco Javier; Huete Toral, Fernando; Rodríguez Pomar, Candela; Martínez Águila, Alejandro; Martín Gil, Alba; Tomé de la Torre, Miguel ÁngelPor cuarto año consecutivo los doctorandos de la Facultad de Óptica y Optometría de la Universidad Complutense de Madrid cuentan con un congreso propio organizado por y para ellos, el 4º PhDAY- FOO. Se trata de un congreso gratuito abierto en la que estos jóvenes científicos podrán presentar sus investigaciones al resto de sus compañeros predoctorales y a toda la comunidad universitaria que quiera disfrutar de este evento. Apunta en tu agenda: el 15 de octubre de 2020. En esta ocasión será un Congreso On-line para evitar que la incertidumbre asociada a la pandemia Covid-19 pudiera condicionar su celebración.Item A Comparative Study of Two Imaging Techniques of Meibomian Glands(Life, 2023) Diz Arias, Elena; Fernández Jiménez, Elena; Peral Cerda, María Asunción; Gómez Pedrero, José AntonioIn the present study, two different meibographers, Oculus Keratograph 5M (K5M) that uses 840 nm infrared light and the Visible Light Non-Contact Meibographer (VLNCM) that uses 610 nm visible light have been used to obtain meibography images from normal and Meibomian Gland Dysfunction (MGD) population. The main objective has been to validate and demonstrate that the use of visible light is useful for observation and quantification of MG in clinical practice. Twenty participants were enrolled in this prospective study. The upper eyelids of one randomly chosen eye were used to obtain results. Forty images were captured and analysed. Three specialized observers were recruited to grade images using Pult and Riede Pult 5-degree scale, in two different sessions. Intra-observer agreement between sessions for both devices was shown. Inter-observer variability analysis showed discrepancy between meiboscores obtained from observers with K5M (p-value < 0.05), except for session 2 in the pathology group, while no statistical difference was found with VLNCM. Repeatability analysis found no statistically significant differences between sessions. Correlation between meibographers showed no statistically significant difference and a moderate correlation coefficient between meiboscores graded with the two devices. The current study suggests that VLNCM can allow MG to be properly visualized and classified in the upper eyelids.- Project number: 260
Item Creación de material audiovisual para el Campus Virtual y plataformas educativas en aras de que los estudiantes de grado, posgrado y profesionales de la visión dominen el análisis ocular con biomicroscopio(2022) Carballo Álvarez, Jesús; Bella Gala, Rafael; Cuiña Sardiña, Ricardo; Durán Prieto, Elena; Peral Cerda, María Asunción; Valverde Megías, Alicia; Cedrún Sánchez, Juan EnriqueEdición y publicación de videos explicando los fundamentos y técnicas del biomicroscopio ocular con el análisis de la salud superficial del polo anterior ocular y las imágenes biomicroscópicas y fluoroscópicas para la correcta adaptación de lentes de contacto. Item Role of instrumental factors in Meibomian gland contrast assessment(Ophthalmic and Physiological Optics, 2023) Diz Arias, Elena; Fernández Jiménez, Elena; Peral Cerda, María Asunción; Gómez Pedrero, José AntonioPurpose: Meibomian gland contrast has been suggested as a potential biomarker in Meibomian gland dysfunction. This study analysed the instrumental factors related to contrast. The objectives were to determine whether the mathematical equations used to compute gland contrast (e.g., Michelson or Yeh and Lin), impact the ability to identify abnormal individuals, to ascertain whether contrast between the gland and the background could be an effective biomarker and to assess whether using contrast-enhancement on the gland image improves its diagnostic efficacy. Methods: A total of 240 meibography images from 40 participants (20 controls and 20 having Meibomian gland dysfunction or blepharitis), were included. The Oculus Keratograph 5M was used to capture images from the upper and lower eyelids of each eye. The contrast of unprocessed images and those pre-processed with contrast-enhancement algorithms were analysed. Contrast was measured on the eight central glands. Two equations for contrast computation were used, and the contrast both between glands and within a gland were calculated. Results: Significant differences were found between the groups for inter-gland area in the upper (p = 0.01) and lower eyelids (p = 0.001) for contrast measured with the Michelson formula. Similar effects were observed when using the Yeh and Lin method in the upper (p = 0.01) and lower eyelids (p = 0.04). These results were obtained for images enhanced with the Keratograph 5M algorithm. Conclusions: Meibomian gland contrast is a useful biomarker of disease related to the Meibomian glands. Contrast measurement should be determined using contrast-enhanced images in the inter-gland area. However, the method used to compute contrast did not influence the results.- Project number: 104
Item Fundamentos y técnicas biomicroscópicas para el análisis del globo ocular y anejos(2021) Carballo Álvarez, Jesús; Cedrún Sánchez, Juan Enrique; Bella Gala, Rafael; Peral Cerda, María Asunción; Cuiña Sardiña, RicardoDescribe las características del Biomicroscopio, también llamado Lámpara de hendidura junto a las diversas técnicas quu se pueden realizar para la observación y estudio de los medios oculares. En el enlace de Youtube siguiente se pueden consultar los videos divulgativos fruto de este Proyecto de innovación realizado en la Facultad de Óptica y Optometría de la UCM Item Oxidative Stress in the Anterior Ocular Diseases: Diagnostic and Treatment(Biomedicines, 2023) Dammak, Azza; Pastrana Robles, Cristina; Martín Gil, Alba; Carpena Torres, Carlos; Peral Cerda, María Asunción; Simovart, Mirjam; Alarma-Estrany, Pilar; Huete Toral, Fernando; Carracedo Rodríguez, Juan GonzaloThe eye is a metabolically active structure, constantly exposed to solar radiations making its structure vulnerable to the high burden of reactive oxygen species (ROS), presenting many molecular interactions. The biomolecular cascade modification is caused especially in diseases of the ocular surface, cornea, conjunctiva, uvea, and lens. In fact, the injury in the anterior segment of the eye takes its origin from the perturbation of the pro-oxidant/antioxidant balance and leads to increased oxidative damage, especially when the first line of antioxidant defence weakens with age. Furthermore, oxidative stress is related to mitochondrial dysfunction, DNA damage, lipid peroxidation, protein modification, apoptosis, and inflammation, which are involved in anterior ocular disease progression such as dry eye, keratoconus, uveitis, and cataract. The different pathologies are interconnected through various mechanisms such as inflammation, oxidative stress making the diagnostics more relevant in early stages. The end point of the molecular pathway is the release of different antioxidant biomarkers offering the potential of predictive diagnostics of the pathology. In this review, we have analysed the oxidative stress and inflammatory processes in the front of the eye to provide a better understanding of the pathomechanism, the importance of biomarkers for the diagnosis of eye diseases, and the recent treatment of anterior ocular diseases.