Person:
Torre Fuentes, Laura

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Laura
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Torre Fuentes
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https://hdl.handle.net/20.500.14352/85788
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Universidad Complutense de Madrid
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Author: Domínguez Rodríguez, Lucas José ×

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    Antimicrobial Susceptibility and Resistance Mechanisms in Mannheimia haemolytica Isolates from Sheep at Slaughter
    (Animals, 2023) Álvarez Sánchez, Julio; Calderón Bernal, Johan M.; Torre Fuentes, Laura; Hernández Martín, Marta María; Pinto Jiménez, Chris E.; Domínguez Rodríguez, Lucas José; Fernández-Garayzábal Fernández, José Francisco; Vela Alonso, Ana Isabel; Cid Vázquez, María Dolores
    Mannheimia haemolytica is the main pathogen contributing to pneumonic pasteurellosis in sheep. The aim of this study was to investigate the antimicrobial resistance levels in M. haemolytica isolates from the lungs of slaughtered sheep and to examine the genetic resistance mechanisms involved. A total of 256 M. haemolytica isolates, 169 from lungs with pneumonic lesions and 87 from lungs without lesions, were analyzed by the disk diffusion method for 12 antimicrobials, and the whole genome of 14 isolates was sequenced to identify antimicrobial resistance determinants. Levels of phenotypic resistance ranged from <2% for 10 antimicrobials (amoxicillin, amoxicillin-clavulanic, ceftiofur, cefquinome, lincomycin/spectinomycin, gentamicin, erythromycin, florfenicol, enrofloxacin, and doxycycline) to 4.3% for tetracycline and 89.1% for tylosin. Six isolates carried tetH genes and four isolates carried, in addition, the strA and sul2 genes in putative plasmid sequences. No mutations associated with macrolide resistance were identified in 23 rDNA sequences, suggesting that the M. haemolytica phenotypic results for tylosin should be interpreted with care in the absence of well-established epidemiological and clinical breakpoints. The identification of strains phenotypically resistant to tetracycline and of several resistance genes, some of which were present in plasmids, highlights the need for continuous monitoring of susceptibility patterns in Pasteurellaceae isolates from livestock.
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    New insights into the pathogenesis and transmission of Brucella pinnipedialis: systemic infection in two bottlenose dolphins (Tursiops truncatus)
    (Microbiology Spectrum, 2023) Vargas Castro, Ignacio; Crespo Picazo, José Luis; Fayos, Manena; Jiménez Martínez, María De Los Ángeles; Torre Fuentes, Laura; Álvarez Sánchez, Julio; Moura, André E.; Hernández, Marta; Buendía Andrés, Aranzazu; Barroso Arévalo, Sandra; García-Seco Romero, María Teresa; Pérez Sancho, Marta; De Miguel, María Jesús; Andrés Barranco, Sara; Marco Cabedo, Vicente; Peñín Villahoz, Gaizka; Muñoz, Pilar María; Domínguez Rodríguez, Lucas José; García Párraga, Daniel; Sánchez-Vizcaíno Rodríguez, José Manuel
    The emergence of Brucella infections in marine mammals is a growing concern. The present study reports two cases of systemic Brucella pinnipedialis infection detected in bottlenose dolphins (Tursiops truncatus) pair stranded together in the Cantabrian coast of Spain. Both animals showed systemic lesions associated with the Brucella infection, more severe in the younger dolphin, considered the likely offspring of the other individual. Real-time PCR, bacterial culture, and whole-genome sequencing were used to detect and characterize the Brucella strains involved in both dolphins. The phylogenetic analysis performed on the Brucella genomes retrieved revealed that the species involved was B. pinnipedialis (ST25). Both animals resulted seropositive in a commercial multispecies blocking ELISA but tested negative in the standard Rose Bengal test. To the best of our knowledge, this is the first report of a systemic infection resulting in various lesions associated with Brucella pinnipedialis (ST25) in two bottlenose dolphins. It is also the initial isolation of Brucella in the milk of a non-pregnant or non-aborting female cetacean likely stranded with its offspring. These findings provide new insights into the epidemiology and clinical impact of B. pinnipedialis infection in cetaceans and underscore the importance of continued diagnostic surveillance to gain better understanding of brucellosis effects and transmission in marine mammal populations.