Person: Sánchez Sánchez, Roberto
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First Name
Roberto
Last Name
Sánchez Sánchez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Identifiers
7 results
Search Results
Now showing 1 - 7 of 7
- Project number: 290
Item Parasitología Interactiva(2016) Gómez Bautista, Mercedes; Ortega Mora, Luis Miguel; Álvarez García, Gema; Ferré Pérez, Ignacio; Moreno Gonzalez, Javier; Gutierrez Expósito, Daniel; Arranz-Solís, David; Pastor Fernández, Iván; Rojo Montejo, Silvia; Sánchez Sánchez, Roberto; García Lunar, Paula; Jiménez Meléndez, Alejandro; Jimenez Pelayo, Laura; García Sánchez, Marta; Diezma Díaz, Carlos; Horcajo Iglesias, María Del Pilar; Regidor Cerrillo, Javier; Pérez Villalobos, Natividad; Collantes Fernández, EstherDesarrollo de una guía interactiva donde se muestran los ciclos biológicos,imágenes y dibujos de los estadios evolutivos de los principales géneros y especies de parásitos protozoos relevantes en al ámbito veterinario. - Project number: 25
Item Parasit`Xpert, un blog de Parasitología Veterinaria(2023) Ferré Pérez, Ignacio; Ortega Mora, Luis Miguel; Álvarez García, Gema; Collantes Fernández, Esther; Horcajo Iglesias, María Del Pilar; Calero Bernal, Rafael; Pastor Fernández, Iván; Arranz-Solís, David; Sánchez Sánchez, Roberto; Regidor Cerrillo, Javier; Hecker, Yanina PaolaParasit’Xpert es un blog sobre enfermedades parasitarias de los rumiantes domésticos para el veterinario (https://parasitxpert.es/). Su objetivo es ser un punto de encuentro y referencia para los profesionales del sector y estudiantes de veterinaria que quieran conocer y profundizar en el campo de las enfermedades parasitarias. La plataforma se inició en enero de 2021 y se estructura en diversas secciones prácticas: parásito del mes, ¿sabías qué?, diagnóstico, control, zoonosis y avances. El grupo SALUVET y la empresa de transferencia del conocimiento universitario SALUVET-Innova han puesto en marcha la web Parasit’xpert junto a la empresa farmacéutica Boehringer Ingelheim Animal Health España. El objetivo general del presente proyecto de innovación docente fue desarrollar el blog Parasit’Xpert (https://parasitxpert.es/) ya en activo e implementar un plan de difusión promocional para incrementar el número de usuarios, especialmente en países hispanohablantes. - Project number: 262
Item Parasitología interactiva: Protozoos y afines(2018) Collantes Fernández, Esther; Gómez Bautista, Mercedes; Ortega Mora, Luis Miguel; Álvarez García, Gema; Ferré Pérez, Ignacio; García Lunar, Paula; Sánchez Sánchez, Roberto; Jiménez Meléndez, Alejandro; García Sánchez, Marta; Jiménez Pelayo, Laura; Diezma Díaz, Carlos; Regidor Cerrillo, Javier; Moreno González, Javier; Horcajo Iglesias, María Del PilarDesarrollo de una guía interactiva donde se muestran los ciclos biológicos, imágenes y dibujos de los estadios evolutivos de los principales géneros y especies de parásitos protozoos relevantes en al ámbito veterinario. Item Assessment of the Activity of Decoquinate and Its Quinoline-O-Carbamate Derivatives against Toxoplasma gondii In Vitro and in Pregnant Mice Infected with T. gondii Oocysts(Molecules, 2021) Ramseier, Jessica; Imhof, Dennis; Anghel, Nicoleta; Hänggeli, Kai; Beteck, Richard M.; Ortega Mora, Luis Miguel; Balmer, Vreni; Sánchez Sánchez, Roberto; Ferré Pérez, Ignacio; Haynes, Richard K.; Hemphill, AndrewThe quinolone decoquinate (DCQ) is widely used in veterinary practice for the treatment of bacterial and parasitic infections, most notably, coccidiosis in poultry and in ruminants. We have investigated the effects of treatment of Toxoplasma gondii in infected human foreskin fibroblasts (HFF) with DCQ. This induced distinct alterations in the parasite mitochondrion within 24 h, which persisted even after long-term (500 nM, 52 days) treatment, although there was no parasiticidal effect. Based on the low half-maximal effective concentration (IC50) of 1.1 nM and the high selectivity index of >5000, the efficacy of oral treatment of pregnant mice experimentally infected with T. gondii oocysts with DCQ at 10 mg/kg/day for 5 days was assessed. However, the treatment had detrimental effects, induced higher neonatal mortality than T. gondii infection alone, and did not prevent vertical transmission. Thus, three quinoline-O-carbamate derivatives of DCQ, anticipated to have better physicochemical properties than DCQ, were assessed in vitro. One such compound, RMB060, displayed an exceedingly low IC50 of 0.07 nM, when applied concomitantly with the infection of host cells and had no impact on HFF viability at 10 µM. As was the case for DCQ, RMB060 treatment resulted in the alteration of the mitochondrial matrix and loss of cristae, but the changes became apparent at just 6 h after the commencement of treatment. After 48 h, RMB060 induced the expression of the bradyzoite antigen BAG1, but TEM did not reveal any other features reminiscent of bradyzoites. The exposure of infected cultures to 300 nM RMB060 for 52 days did not result in the complete killing of all tachyzoites, although mitochondria remained ultrastructurally damaged and there was a slower proliferation rate. The treatment of mice infected with T. gondii oocysts with RMB060 did reduce parasite burden in non-pregnant mice and dams, but vertical transmission to pups could not be prevented.Item A questionnaire-based survey in Spain provides relevant information to improve the control of ovine coccidiosis(Frontiers in Veterinary Science, 2023) Sánchez Sánchez, Roberto; Gutiérrez, Jorge; Blasco Castello, José Luis; Marcos Santamaría, María; Cano Alsua, Santiago; Elvira, Laura; Ferré Pérez, Ignacio; Ortega Mora, Luis MiguelOvine coccidiosis is a widespread intestinal parasitic disease caused by Eimeria spp. Lambs are infected by the ingestion of sporulated oocysts, experiencing diarrhea and low growth rates. Control should be based on measures to reduce infection pressure and stress on the animals as well as on appropriate diagnosis and strategic treatment. To obtain information on how control measures are implemented in the ovine sector in Spain, a questionnaire-based survey was completed in 2022 by 154 veterinarians and 173 farmers working in this sector. Coccidiosis was highlighted as a relevant disease by 34% of the respondents. The period of greatest risk seemed to differ between production systems, being mainly early after weaning (7–15 days after weaning) in meat flocks and feedlots and later (1–2 months after weaning) in dairy flocks. The absence of cleaning and disinfection measures was identified as a risk factor by 51% of the veterinarians, with 22% mentioning overcrowding of animals and 22% indicating that coccidiosis has more incidence in flocks with large number of animals. The use of laboratory diagnosis methods (fecal oocyst count) was unusual in 70 and 84% of the veterinarians and farmers, respectively. Regarding control, dairy flocks usually housed a larger number of animals under intensive conditions, and they implemented more frequently control measures for coccidiosis than meat flocks. Anticoccidial drugs were used in 79% of the flocks, and in 74–82% of them, they were applied based on clinical criteria. Comparing protocols for anticoccidial treatment among different production systems, in meat flocks, anticoccidial drugs were applied more frequently when clinical signs were observed, and coccidiostats were used for less than 28 days compared to dairy flocks. These results highlight the need for improvement in the use of anticoccidial treatments adjusted to the new regulatory framework in the EU, which in turn will rationalize the use of antimicrobial compounds and may help to mitigate the impact of coccidiosis in flocks.Item An Early Treatment With BKI-1748 Exhibits Full Protection Against Abortion and Congenital Infection in Sheep Experimentally Infected With Toxoplasma gondii(The Journal of Infectious Diseases, 2023) Sánchez Sánchez, Roberto; Imhof, Dennis; Hecker, Yanina Paola; Ferré Pérez, Ignacio; Re, Michela Tatiana; Moreno Gonzalo, Javier; Blanco Murcia, Francisco Javier; Mejías López, Elena; Hulverson, Matthew A.; Choi, Ryan; Arnold, Samuel L. M.; Ojo, Kayode K.; Barrett, Lynn K.; Hemphill, Andrew; Van Voorhis, Wesley C.; Ortega Mora, Luis MiguelCongenital toxoplasmosis in humans and in other mammalian species, such as small ruminants, is a well-known cause of abortion and fetal malformations. The calcium-dependent protein kinase 1 (CDPK1) inhibitor BKI-1748 has shown a promising safety profile for its use in humans and a good efficacy against Toxoplasma gondii infection in vitro and in mouse models. Ten doses of BKI-1748 given every other day orally in sheep at 15 mg/kg did not show systemic or pregnancy-related toxicity. In sheep experimentally infected at 90 days of pregnancy with 1000 TgShSp1 oocysts, the BKI-1748 treatment administered from 48 hours after infection led to complete protection against abortion and congenital infection. In addition, compared to infected/untreated sheep, treated sheep showed a drastically lower rectal temperature increase and none showed IgG seroconversion throughout the study. In conclusion, BKI-1748 treatment in pregnant sheep starting at 48 hours after infection was fully effective against congenital toxoplasmosis.Item A short-term treatment with BKI-1294 does not protect foetuses from sheep experimentally infected with Neospora caninum tachyzoites during pregnancy(International Journal for Parasitology: Drugs and Drug Resistance, 2021) Sánchez Sánchez, Roberto; Ferré Pérez, Ignacio; Re, Michela Tatiana; Pérez Arroyo, Bárbara; Cleofé Resta, Darío; Herrero García, Victor; Pizarro Díaz, Manuel; Ferrer, Luis Miguel; Ruiz, Hector; Vallejo García, Raquel; Benavides, Julio; Hulverson, Matthew A.; Choi, Ryan; Whitman, Grant R.; Hemphill, Andrew; Van Voorhis, Wesley C.; Ortega Mora, Luis MiguelThe Neospora caninum Calcium-dependent protein kinase 1 (NcCDPK1) inhibitor BKI-1294 had demonstrated excellent efficacy in a pregnant mouse model of neosporosis, and was also highly efficacious in a pregnant sheep model of toxoplasmosis. In this work, we present the efficacy of BKI-1294 treatment (dosed 5 times orally every 48 h) starting 48 h after intravenous infection of sheep with 105 Nc-Spain7 tachyzoites at mid-pregnancy. In the dams, BKI-1294 plasma concentrations were above the IC50 for N. caninum for 12–15 days. In treated sheep, when they were compared to untreated ones, we observed a minor increase in rectal temperature, higher IFNγ levels after blood stimulation in vitro, and a minor increase of IgG levels against N. caninum soluble antigens through day 28 post-infection. Additionally, the anti-NcSAG1 and anti-NcSAG4 IgGs were lower in treated dams on days 21 and 42 post-infection. However, BKI-1294 did not protect against abortion (87% foetal mortality in both infected groups, treated and untreated) and did not reduce transplacental transmission, parasite load or lesions in placentomes and foetal brain. The lack of foetal protection was likely caused by short systemic exposure in the dams and suboptimal foetal exposure to this parasitostatic drug, which was unable to reduce replication of the likely established N. caninum tachyzoites in the foetus at the moment of treatment. New BKIs with a very low plasma clearance and good ability to cross the blood-brain and placental barriers need to be developed.