Person: Sánchez Ramos, Celia
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First Name
Celia
Last Name
Sánchez Ramos
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
Identifiers
3 results
Search Results
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Item Removal of the blue component of light significantly decreases retinal damage after high intensity exposure(PLoS ONE, 2018) Vicente-Tejedor, Javier; Marchena, Miguel; Ramírez, Laura; García Ayuso, Diego; Gómez Vicente, Violeta; Sánchez Ramos, Celia; Villa Polo, Pedro de la; Germain, FranciscoLight causes damage to the retina (phototoxicity) and decreases photoreceptor responses to light. The most harmful component of visible light is the blue wavelength (400–500 nm). Different filters have been tested, but so far all of them allow passing a lot of this wavelength (70%). The aim of this work has been to prove that a filter that removes 94% of the blue component may protect the function and morphology of the retina significantly. Three experimental groups were designed. The first group was unexposed to light, the second one was exposed and the third one was exposed and protected by a blue-blocking filter. Light damage was induced in young albino mice (p30) by exposing them to white light of high intensity (5,000 lux) continuously for 7 days. Short wavelength light filters were used for light protection. The blue component was removed (94%) from the light source by our filter. Electroretinographical recordings were performed before and after light damage. Changes in retinal structure were studied using immunohistochemistry, and TUNEL labeling. Also, cells in the outer nuclear layer were counted and compared among the three different groups. Functional visual responses were significantly more conserved in protected animals (with the blue-blocking filter) than in unprotected animals. Also, retinal structure was better kept and photoreceptor survival was greater in protected animals, these differences were significant in central areas of the retina. Still, functional and morphological responses were significantly lower in protected than in unexposed groups. In conclusion, this blue-blocking filter decreases significantly photoreceptor damage after exposure to high intensity light. Actually, our eyes are exposed for a very long time to high levels of blue light (screens, artificial light LED, neons…). The potential damage caused by blue light can be palliated.Item Effects of Light‐emitting Diode Radiations on Human Retinal Pigment Epithelial Cells in vitro(Photochemistry and Photobiology, 2012) Chamorro, Eva; Muñoz de Luna, Javier; Bonnin Arias, Cristina Natalia; Pérez Carrasco, María Jesús; Vázquez Molini, Daniel; Sánchez Ramos, CeliaHuman visual system is exposed to high levels of natural and artificial lights of different spectra and intensities along lifetime. Light-emitting diodes (LEDs) are the basic lighting components in screens of PCs, phones and TV sets; hence it is so important to know the implications of LED radiations on the human visual system. The aim of this study was to investigate the effect of LEDs radiations on human retinal pigment epithelial cells (HRPEpiC). They were exposed to three light-darkness (12 h/12 h) cycles, using blue-468 nm, green-525 nm, red-616 nm and white light. Cellular viability of HRPEpiC was evaluated by labeling all nuclei with DAPI; Production of reactive oxygen species (ROS) was determined by H2DCFDA staining; mitochondrial membrane potential was quantified by TMRM staining; DNA damage was determined by H2AX histone activation, and apoptosis was evaluated by caspases-3,-7 activation. It is shown that LED radiations decrease 75-99% cellular viability, and increase 66-89% cellular apoptosis. They also increase ROS production and DNA damage. Fluorescence intensity of apoptosis was 3.7% in nonirradiated cells and 88.8%, 86.1%, 83.9% and 65.5% in cells exposed to white, blue, green or red light, respectively. This study indicates three light-darkness (12 h/12 h) cycles of exposure to LED lighting affect in vitro HRPEpiC.Item New Optical Filter Plate for Use as Eye Protection by Welders(Welding journal, 2013) Langa Moraga, Antonio; Bonnin Arias, Cristina Natalia; Chamorro, Eva; Pérez Carrasco, María Jesús; Sánchez Ramos, CeliaPeople whose work tasks involve the use of welding torches are at special risk of suffering eye injuries due to the emission of visible, short-wavelength radiation. Current legislation requires that a company provide its employees with protection against the harmful radiation produced by welding equipment. Often, however, a worker will be reluctant to use protective goggles since these markedly cut visibility and can consequently lead to errors or even burns. This practice of avoiding the use of protection makes them susceptible to suffer irreversible severe retinal damage leading to partial or complete loss of vision. In this paper, we propose the use of a new photoprotective filter in the form of safety goggles that seeks to improve the compromised vision produced by conventional filters. We compare a series of visual function variables in 36 adults, aged 30 to 58 years, using the new optical filter and a conventional filter used for welding protection. Our findings suggest that the filter proposed provides optimal protection against the harmful effects of short-wavelength radiation while minimizing the reduced vision effects of conventional filters used for this purpose.