Person:
Rancán, Lisa

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First Name
Lisa
Last Name
Rancán
URI
https://hdl.handle.net/20.500.14352/79005
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Bioquímica y Biología Molecular
Area
Bioquímica y Biología Molecular
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    Differences in platelet‐rich plasma composition influence bone healing
    (Journal of Clinical Periodontology, 2021) Al‐Hamed,; Abu‐Nada, Lina; Rodan, Rania; Sarrigiannidis, Stylianos; Ramirez‐Garcialuna, Jose Luis; Moussa, Hanan; Elkashty, Osama; Gao, Qiman; Tayebeh Basiri; Baca, Laura; Torres García Denche, Jesús; Rancán, Lisa; Simon D. Tran; Marie Lordkipanidzé; Mari Kaartinen; Zahi Badran; Faleh Tamimi
    Aim: Platelet-rich plasma (PRP) is an autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP (L-PRP) composition on bone defect healing. Materials and Methods: L-PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP, double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro-CT and histology. Results: Double-spin L-PRP contained higher concentrations of platelets than singlespin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08 ± 0.2 mm3) compared to single-spin L-PRP (1.42 ± 0.27 mm3) or filtered L-PRP (1.38 ± 0.28 mm3). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double-spin L-PRP than in those treated with single-spin or filtered L-PRP. Conclusion: The preparation method of L-PRP affects their composition and potential to regenerate bone.