Person:
Guevara Acosta, Flor Govinda

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First Name
Flor Govinda
Last Name
Guevara Acosta
URI
https://hdl.handle.net/20.500.14352/74397
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Department
Bioquímica y Biología Molecular
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Author: Navarro Llorens, Juana María × Subject: 577.175.62 ×

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    Metabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone production
    (Plos One, 2019) Guevara Acosta, Flor Govinda; Olortegui Flores, Yamileth; Fernández de las Heras, Laura; Perera González, Julián; Navarro Llorens, Juana María
    Rhodococcus ruber Chol-4 is a potent steroid degrader that has a great potential as a biotechnological tool. As proof of concept, this work presents testosterone production from 4- androstene-3,17-dione by tailoring innate catabolic enzymes of the steroid catabolism inside the strain. A R. ruber quadruple mutant was constructed in order to avoid the breakage of the steroid nucleus. At the same time, an inducible expression vector for this strain was developed. The 17-ketoreductase gene from the fungus Cochliobolus lunatus was cloned and overexpressed in this vector. The engineered strain was able to produce testosterone from 4-androstene-3,17-dione using glucose for cofactor regeneration with a molar conversion of 61%. It is important to note that 91% of the testosterone was secreted outside the cell after 3 days of cell biotransformation. The results support the idea that Rhodococcus ruber Chol-4 can be metabolically engineered and can be used for the production of steroid intermediates.