Person:
Cuadrado Vives, María Carmen

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First Name
María Carmen
Last Name
Cuadrado Vives
URI
https://hdl.handle.net/20.500.14352/77721
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Nutrición y Ciencia de los Alimentos
Area
Nutrición y Bromatología
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20171
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Author: Ballesteros Redondo, María Isabel × Subject: 577.213.3.08 ×

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    Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches
    (Food Control, 2017) Sanchiz Giraldo, África; Ballesteros Redondo, María Isabel; Martín, A.; Rueda Muñoz de San Pedro, Julia; Martín Pedrosa, Mercedes; Diéguez, Carmen; Rovira, Mercè; Cuadrado Vives, María Carmen; Linacero De La Fuente, M. Rosario
    The labelled of pistachio on food products is mandatory and, as a consequence, the development of suitable analytical methodologies to detect this nut in processed foods is advisable. In this work, two different qPCR assays to detect pistachio, SYBR®Green and locked nucleic acid (LNA) probes, are tested and compared. Pis v allergen coding sequences have been amplified and cloned in different pistachio varieties, and specific primers and probes for each allergen have been designed. According to our results, LNA probe-real time PCR appears to be the most sensitive and specific method, reaching 10 mg/kg of pistachio. The effect of temperature and/or pressure on pistachio DNA detection was also analysed by LNA probe-based qPCR. Data showed a reduced amplificability of pistachio after thermal treatment under pressure, nevertheless, this effect was not observed after boiling. The applicability of this method has been studied by analysing 14 food products and by comparison with a commercial ELISA kit.