Person:
Cuadrado Vives, María Carmen

Profile Picture
First Name
María Carmen
Last Name
Cuadrado Vives
URI
https://hdl.handle.net/20.500.14352/77721
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Nutrición y Ciencia de los Alimentos
Area
Nutrición y Bromatología
Identifiers
UCM identifierORCIDScopus Author IDDialnet ID

Filters

2015 - 20173
Reset filters

Settings

Author: Martín Pedrosa, Mercedes ×

Search Results

Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches
    (Food Control, 2017) Sanchiz Giraldo, África; Ballesteros Redondo, María Isabel; Martín, A.; Rueda Muñoz de San Pedro, Julia; Martín Pedrosa, Mercedes; Diéguez, Carmen; Rovira, Mercè; Cuadrado Vives, María Carmen; Linacero De La Fuente, M. Rosario
    The labelled of pistachio on food products is mandatory and, as a consequence, the development of suitable analytical methodologies to detect this nut in processed foods is advisable. In this work, two different qPCR assays to detect pistachio, SYBR®Green and locked nucleic acid (LNA) probes, are tested and compared. Pis v allergen coding sequences have been amplified and cloned in different pistachio varieties, and specific primers and probes for each allergen have been designed. According to our results, LNA probe-real time PCR appears to be the most sensitive and specific method, reaching 10 mg/kg of pistachio. The effect of temperature and/or pressure on pistachio DNA detection was also analysed by LNA probe-based qPCR. Data showed a reduced amplificability of pistachio after thermal treatment under pressure, nevertheless, this effect was not observed after boiling. The applicability of this method has been studied by analysing 14 food products and by comparison with a commercial ELISA kit.
  • Thumbnail Image
    Item
    Determination of B-N-oxalyl-L-a, B-diaminopropionic acid and homoarginine in Lathyrus sativus and Lathyrus cicera by capillary zone electrophoresis
    (Journal of the Science of Food and Agriculture, 2015) Sacristán San Cristóbal, Mara; Varela, Alejandro; Martín Pedrosa, Mercedes; Burbano, Carmen; Cuadrado Vives, María Carmen; Legaz González, María Estrella; Muzquiz, Mercedes
    BACKGROUND: Lathyrus species as legumes represent an alternative protein source for human and animal nutrition. Heavy consumption of these species can lead to lathyrism, caused by the non-protein amino acid B-N-oxalyl-L-a, B-diaminopropionic acid (B-ODAP). Currently, there is no well-defined level below which B-ODAP is considered non-toxic. In this work, the B-ODAP content was determined in L. sativus and L. cicera samples to assess their potential toxicity. Homoarginine is another non-protein amino acid found in Lathyrus spp. with interesting implications for human and animal nutrition. RESULTS: The level of B-ODAP found in these two species ranged from 0.79 to 5.05 mg g−1. The homoarginine content of the samples ranged from 7.49 to 12.44 mg g−1. CONCLUSION: This paper describes an accurate, fast and sensitive method of simultaneous detection and quantification of B-ODAP and homoarginine by capillary zone electrophoresis in L. cicera and L. sativus seeds. Moreover, several methods of extraction were compared to determine the highest performance.
  • Thumbnail Image
    Item
    Detection by real time PCR of walnut allergen coding sequences in processed foods
    (Food Chemistry, 2016) Linacero De La Fuente, M. Rosario; Ballesteros Redondo, María Isabel; Sanchiz Giraldo, África; Prieto, Nuria; Iniesto, Elisa; Martínez, Yolanda; Martín Pedrosa, Mercedes; Muzquiz, Mercedes; Cabanillas Martín, Beatriz; Rovira, Mercè; Burbano, Carmen; Cuadrado Vives, María Carmen
    A quantitative real-time PCR (RT-PCR) method, employing novel primer sets designed on Jug r 1, Jug r 3, and Jug r 4 allergen-coding sequences, was set up and validated. Its specificity, sensitivity, and applicability were evaluated. The DNA extraction method based on CTAB–phenol–chloroform was best for walnut. RT-PCR allowed a specific and accurate amplification of allergen sequence, and the limit of detection was 2.5 pg of walnut DNA. The method sensitivity and robustness were confirmed with spiked samples, and Jug r 3 primers detected up to 100 mg/kg of raw walnut (LOD 0.01%, LOQ 0.05%). Thermal treatment combined with pressure (autoclaving) reduced yield and amplification (integrity and quality) of walnut DNA. High hydrostatic pressure (HHP) did not produce any effect on the walnut DNA amplification. This RT-PCR method showed greater sensitivity and reliability in the detection of walnut traces in commercial foodstuffs compared with ELISA assays.