Person:
Colilla Nieto, Montserrat

First Name

Montserrat

Last Name

Colilla Nieto

URI

https://hdl.handle.net/20.500.14352/77764

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Farmacia

Department

Química en Ciencias Farmacéuticas

Area

Química Inorgánica

Identifiers

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Search Results

Now showing 1 - 8 of 8

Magnetic colloidal nanoformulations to remotely trigger mechanotransduction for osteogenic differentiation
(Journal of Colloid And Interface Science, 2024) Gutiérrez Estévez, Manuel; Cicuéndez Maroto, Mónica; Colilla Nieto, Montserrat; Vallet Regí, María Dulce Nombre; González Ortiz, Blanca; Izquierdo Barba, Isabel
Nowadays, diseases associated with an ageing population, such as osteoporosis, require the development of new biomedical approaches to bone regeneration. In this regard, mechanotransduction has emerged as a discipline within the field of bone tissue engineering. Herein, we have tested the efficacy of superparamagnetic iron oxide nanoparticles (SPIONs), obtained by the thermal decomposition method, with an average size of 13 nm, when exposed to the application of an external magnetic field for mechanotransduction in human bone marrowderived mesenchymal stem cells (hBM-MSCs). The SPIONs were functionalized with an Arg-Gly-Asp (RGD) peptide as ligand to target integrin receptors on cell membrane and used in colloidal state. Then, a comprehensive and comparative bioanalytical characterization of non-targeted versus targeted SPIONs was performed in terms of biocompatibility, cell uptake pathways and mechanotransduction effect, demonstrating the osteogenic
Mesoporous Silica Nanoparticles Decorated with Polycationic Dendrimers for Infection Treatment
(Acta Biomaterialia, 2018) González Ortiz, Blanca; Colilla Nieto, Montserrat; Díez, Jaime; Pedraza, Daniel; Guembe, Marta; Izquierdo Barba, Isabel; Vallet Regí, María Dulce Nombre
This work aims to provide an effective and novel solution for the treatment of infection by using nanovehicles loaded with antibiotics capable of penetrating the bacterial wall, thus increasing the antimicrobial effectiveness. These nanosystems, named "nanoantibiotics", are composed of mesoporous silica nanoparticles (MSNs), which act as nanocarriers of an antimicrobial agent (levofloxacin, LEVO) localized inside the mesopores. To provide the nanosystem of bacterial membrane interaction capability, a polycationic dendrimer, concretely the poly(propyleneimine) dendrimer of third generation (G3), was covalently grafted to the external surface of the LEVOloaded MSNs. After physicochemical characterization of this nanoantibiotic, the release kinetics of LEVO and the antimicrobial efficacy of each released dosage were evaluated. Besides, internalization studies of the MSNs functionalized with the G3 dendrimer were carried out, showing a high penetrability throughout Gram-negative bacterial membranes. This work evidences that the synergistic combination of polycationic dendrimers as bacterial membrane permeabilization agents with LEVO-loaded MSNs triggers an efficient antimicrobial effect on Gram-negative bacterial biofilm. These positive results open up very promising expectations for their potential application in new infection therapies.
Nanoantibiotics Based in Mesoporous Silica Nanoparticles: New Formulations for Bacterial Infection Treatment.
(Pharmaceutics, 2021) Álvarez Corchado, Elena; González Ortiz, Blanca; Lozano Borregón, Daniel; Doadrio Villarejo, Antonio Luis; Colilla Nieto, Montserrat; Izquierdo Barba, Isabel
This review focuses on the design of mesoporous silica nanoparticles for infection treat‐ment. Written within a general context of contributions in the field, this manuscript highlights the major scientific achievements accomplished by Prof. Vallet‐Regí’s research group in the field of silica‐based mesoporous materials for drug delivery. The aim is to bring out her pivotal role on the envisage of a new era of nanoantibiotics by using a deep knowledge on mesoporous materials as drug delivery systems and applying cutting‐edge technologies to design and engineer advanced nanoweapons to fight infection. This review has been divided in two main sections: the first part overviews the influence of the textural and chemical properties of silica‐based mesoporous materials on the loading and release of antibiotic molecules, depending on the host‐guest interactions.Furthermore, this section also remarks the potential of molecular modelling in the design and comprehension of the performance of these release systems. The second part describes the more recent advances in the use of mesoporous silica nanoparticles as versatile nanoplatforms for the development of novel targeted and stimuli‐responsive antimicrobial nanoformulations for future application in personalized infection therapies.
Large-scale production of superparamagnetic iron oxide nanoparticles by flame spray pyrolysis: in vitro biological evaluation for biomedical applications
(Journal of Colloid And Interface Science, 2023) Gutiérrez Estévez, Manuel; Cicuéndez Maroto, Mónica; Crespo, Julián; Serrano-López, Juana; Colilla Nieto, Montserrat; Fernández-Acevedo, Claudio; Oroz-Mateo, Tamara; Rada-Leza, Amaia; González Ortiz, Blanca; Izquierdo Barba, Isabel; Vallet Regí, María Dulce Nombre
Despite the large number of synthesis methodologies described for superparamagnetic iron oxide nanoparticles (SPIONs), the search for their large-scale production for their widespread use in biomedical applications remains a mayor challenge. Flame Spray Pyrolysis (FSP) could be the solution to solve this limitation, since it allows the fabrication of metal oxide nanoparticles with high production yield and low manufacture costs. However, to our knowledge, to date such fabrication method has not been upgraded for biomedical purposes. Herein, SPIONs
A versatile multicomponent mesoporous silica nanosystem with dual antimicrobial and osteogenic effects.
(Acta Biomaterialia, 2021) Alvarez Corchado, Elena; Estévez Amado, Manuel; Jiménez Jiménez, Carla; Colilla Nieto, Montserrat; Izquierdo Barba, Isabel; González Ortiz, Blanca; Vallet Regí, María Dulce Nombre
In this manuscript, we propose a simple and versatile methodology to design nanosystems based on biocompatible and multicomponent mesoporous silica nanoparticles (MSNs) for infection management. This strategy relies on the combination of antibiotic molecules and antimicrobial metal ions into the same nanosystem, affording a significant improvement of the antibiofilm effect compared to that of nanosystems carrying only one of these agents. The multicomponent nanosystem is based on MSNs externally functionalized with a polyamine dendrimer (MSN-G3) that favors internalization inside the bacteria and allows the complexation of multiactive metal ions (MSN-G3-M n+). Importantly, the selection of both the antibiotic and the cation may be done depending on clinical needs. Herein, levofloxacin and Zn2+ ion, chosen owing to both its antimicrobial and osteogenic capability, have been incorporated. This dual biological role of Zn2+ could have and adjuvant effect thought destroying the biofilm in combination with the antibiotic as well as aid to the repair and regeneration of lost bone tissue associated to osteolysis during infection process. The versatility of the nanosystem has been demonstrated incorporating Ag+ ions in a reference nanosystem. In vitro antimicrobial assays in planktonic and biofilm state show a high antimicrobial efficacy due to the combined action of levofloxacin and Zn2+, achieving an antimicrobial efficacy above 99% compared to the MSNs containing only one of the microbicide agents. In vitro cell cultures with MC3T3-E1 preosteoblasts reveal the osteogenic capability of the nanosystem, showing a positive effect on osteoblastic differentiation while preserving the cell viability.
Superparamagnetic iron oxide nanoparticles decorated mesoporous silica nanosystem for combined antibiofilm therapy
(Pharmaceutics, 2023) Álvarez Corchado, Elena; Estévez Amado, Manuel; Gallo Cordova, Álvaro; González Ortiz, Blanca; Castillo, Rafael R.; Morales, María del Puerto; Colilla Nieto, Montserrat; Izquierdo Barba, Isabel; Vallet Regí, María Dulce Nombre
A crucial challenge to face in the treatment of biofilm-associated infection is the ability of bacteria to develop resistance to traditional antimicrobial therapies based on the administration of antibiotics alone. This study aims to apply magnetic hyperthermia together with controlled antibiotic delivery from a unique magnetic-responsive nanocarrier for a combination therapy against biofilm. The design of the nanosystem is based on antibiotic-loaded mesoporous silica nanoparticles (MSNs) externally functionalized with a thermo-responsive polymer capping layer, and decorated in the outermost surface with superparamagnetic iron oxide nanoparticles (SPIONs). The SPIONs are able to generate heat upon application of an alternating magnetic field (AMF), reaching the temperature needed to induce a change in the polymer conformation from linear to globular, therefore triggering pore uncapping and the antibiotic cargo release. The microbiological assays indicated that exposure of E. coli biofilms to 200 µg/mL of the nanosystem and the application of an AMF (202 kHz, 30 mT) decreased the number of viable bacteria by 4 log10 units compared with the control. The results of the present study show that combined hyperthermia and antibiotic treatment is a promising approach for the effective management of biofilm-associated infections.
Squaraine-loaded mesoporous silica nanoparticles for antimicrobial Photodynamic Therapy against bacterial infection
(Microporous and Mesoporous Materials, 2024) Melese Dereje, Degnet; García Fontecha, Ana; Pontremoli, Carlotta; González Ortiz, Blanca; Colilla Nieto, Montserrat; Vallet Regí, María Dulce Nombre; Izquierdo Barba, Isabel; Barbero, Nadia
Antimicrobial photodynamic therapy (aPDT) shows promise as a complementary or alternative approach to conventional antimicrobial treatments. Despite possessing some key advantages, many challenges remain, such as optimizing the delivery of photosensitizers, improving light penetration into tissues, and determining the most effective combinations of photosensitizers and light wavelengths for different infections. Moreover, addressing the challenges associated with the aggregation tendency and poor solubility of some photosensitizers, squaraine dyes (SQs) in particular, is crucial for unlocking their full potential in biomedical applications. This contribution focuses on designing innovative anophotosensitizers with antimicrobial properties using mesoporous silica nanoparticles (MSNs) loaded with a SQ dye (i.e. Br-SQ). MSNs before and after Br-SQ loading were deeply characterized using different techniques, proving the successful incorporation of the dye into the nanocarriers. Upon visible light (640 nm) irradiation, these nanosystems demonstrated remarkable antibacterial activities against both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli). Our results confirmed that MSNs are valuable nanocarriers of hydrophobic photosensitizers, such as Br-SQ, bringing up new opportunities to develop antibiotic-free anoformulations to treat bacterial infection while minimizing the risk of antimicrobial resistance.
Poly Lactic-co-Glycolic Acid (PLGA) Loaded with a Squaraine Dye as Photosensitizer for Antimicrobial Photodynamic Therapy
(Polymers, 2024) Melese Dereje, Degnet; Pontremoli, Carlotta; García Fontecha, Ana; Galliano, Simone; Colilla Nieto, Montserrat; González Ortiz, Blanca; Vallet Regí, María Dulce Nombre; Izquierdo Barba, Isabel; Barbero, Nadia
Antimicrobial Photodynamic Therapy (aPDT) is an innovative and promising method for combating infections, reducing the risk of antimicrobial resistance compared to traditional antibiotics. Squaraine (SQ) dyes can be considered promising photosensitizers (PSs) but are generally hydrophobic molecules that can self-aggregate under physiological conditions. To overcome these drawbacks, a possible solution is to incorporate SQs inside nanoparticles (NPs). The present work deals with the design and development of innovative nanophotosensitizers based on poly lactic-co-glycolic acid (PLGA) NPs incorporating a brominated squaraine (BrSQ) with potential application in aPDT. Two designs of experiments (DoEs) based on the single emulsion and nanoprecipitation methods were set up to investigate how different variables (type of solvent, solvent ratio, concentration of PLGA, stabilizer and dye, sonication power and time) can affect the size, zeta (ζ)-potential, yield, entrapment efficiency, and drug loading capacity of the SQ-PLGA NPs. SQ-PLGA NPs were characterized by NTA, FE-SEM, and UV-Vis spectroscopy and the ability to produce reactive oxygen species (ROS) was evaluated, proving that ROS generation ability is preserved in SQ-PLGA. In vitro antimicrobial activity against Gram-positive bacteria in planktonic state using Staphylococcus aureus was conducted in different conditions and pH to evaluate the potential of these nanophotosensitizers for aPDT in the local treatment of infections.