Person: Colilla Nieto, Montserrat
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First Name
Montserrat
Last Name
Colilla Nieto
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Química en Ciencias Farmacéuticas
Area
Química Inorgánica
Identifiers
4 results
Search Results
Now showing 1 - 4 of 4
Item Magnetic colloidal nanoformulations to remotely trigger mechanotransduction for osteogenic differentiation(Journal of Colloid And Interface Science, 2024) Gutiérrez Estévez, Manuel; Cicuéndez Maroto, Mónica; Colilla Nieto, Montserrat; Vallet Regí, María Dulce Nombre; González Ortiz, Blanca; Izquierdo Barba, IsabelNowadays, diseases associated with an ageing population, such as osteoporosis, require the development of new biomedical approaches to bone regeneration. In this regard, mechanotransduction has emerged as a discipline within the field of bone tissue engineering. Herein, we have tested the efficacy of superparamagnetic iron oxide nanoparticles (SPIONs), obtained by the thermal decomposition method, with an average size of 13 nm, when exposed to the application of an external magnetic field for mechanotransduction in human bone marrowderived mesenchymal stem cells (hBM-MSCs). The SPIONs were functionalized with an Arg-Gly-Asp (RGD) peptide as ligand to target integrin receptors on cell membrane and used in colloidal state. Then, a comprehensive and comparative bioanalytical characterization of non-targeted versus targeted SPIONs was performed in terms of biocompatibility, cell uptake pathways and mechanotransduction effect, demonstrating the osteogenicItem Organically Modified Mesoporous Silica Nanoparticles against Bacterial Resistance(Chemistry of Materials, 2023) Colilla Nieto, Montserrat; Vallet Regí, María Dulce NombreBacterial antimicrobial resistance is posed to become a major hazard to global health in the 21st century. An aggravating issue is the stalled antibiotic research pipeline, which requires the development of new therapeutic strategies to combat antibiotic-resistant infections. Nanotechnology has entered into this scenario bringing up the opportunity to use nanocarriers capable of transporting and delivering antimicrobials to the target site, overcoming bacterial resistant barriers. Among them, mesoporous silica nanoparticles (MSNs) are receiving growing attention due to their unique features, including large drug loading capacity, biocompatibility, tunable pore sizes and volumes, and functionalizable silanol-rich surface. This perspective article outlines the recent research advances in the design and development of organically modified MSNs to fight bacterial infections. First, a brief introduction to the different mechanisms of bacterial resistance is presented. Then, we review the recent scientific approaches to engineer multifunctional MSNs conceived as an assembly of inorganic and organic building blocks, against bacterial resistance. These elements include specific ligands to target planktonic bacteria, intracellular bacteria, or bacterial biofilm; stimuli-responsive entities to prevent antimicrobial cargo release before arriving at the target; imaging agents for diagnosis; additional constituents for synergistic combination antimicrobial therapies; and aims to improve the therapeutic outcomes. Finally, this manuscript addresses the current challenges and future perspectives on this hot research area.Item Squaraine-loaded mesoporous silica nanoparticles for antimicrobial Photodynamic Therapy against bacterial infection(Microporous and Mesoporous Materials, 2024) Melese Dereje, Degnet; García Fontecha, Ana; Pontremoli, Carlotta; González Ortiz, Blanca; Colilla Nieto, Montserrat; Vallet Regí, María Dulce Nombre; Izquierdo Barba, Isabel; Barbero, NadiaAntimicrobial photodynamic therapy (aPDT) shows promise as a complementary or alternative approach to conventional antimicrobial treatments. Despite possessing some key advantages, many challenges remain, such as optimizing the delivery of photosensitizers, improving light penetration into tissues, and determining the most effective combinations of photosensitizers and light wavelengths for different infections. Moreover, addressing the challenges associated with the aggregation tendency and poor solubility of some photosensitizers, squaraine dyes (SQs) in particular, is crucial for unlocking their full potential in biomedical applications. This contribution focuses on designing innovative anophotosensitizers with antimicrobial properties using mesoporous silica nanoparticles (MSNs) loaded with a SQ dye (i.e. Br-SQ). MSNs before and after Br-SQ loading were deeply characterized using different techniques, proving the successful incorporation of the dye into the nanocarriers. Upon visible light (640 nm) irradiation, these nanosystems demonstrated remarkable antibacterial activities against both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli). Our results confirmed that MSNs are valuable nanocarriers of hydrophobic photosensitizers, such as Br-SQ, bringing up new opportunities to develop antibiotic-free anoformulations to treat bacterial infection while minimizing the risk of antimicrobial resistance.Item Poly Lactic-co-Glycolic Acid (PLGA) Loaded with a Squaraine Dye as Photosensitizer for Antimicrobial Photodynamic Therapy(Polymers, 2024) Melese Dereje, Degnet; Pontremoli, Carlotta; García Fontecha, Ana; Galliano, Simone; Colilla Nieto, Montserrat; González Ortiz, Blanca; Vallet Regí, María Dulce Nombre; Izquierdo Barba, Isabel; Barbero, NadiaAntimicrobial Photodynamic Therapy (aPDT) is an innovative and promising method for combating infections, reducing the risk of antimicrobial resistance compared to traditional antibiotics. Squaraine (SQ) dyes can be considered promising photosensitizers (PSs) but are generally hydrophobic molecules that can self-aggregate under physiological conditions. To overcome these drawbacks, a possible solution is to incorporate SQs inside nanoparticles (NPs). The present work deals with the design and development of innovative nanophotosensitizers based on poly lactic-co-glycolic acid (PLGA) NPs incorporating a brominated squaraine (BrSQ) with potential application in aPDT. Two designs of experiments (DoEs) based on the single emulsion and nanoprecipitation methods were set up to investigate how different variables (type of solvent, solvent ratio, concentration of PLGA, stabilizer and dye, sonication power and time) can affect the size, zeta (ζ)-potential, yield, entrapment efficiency, and drug loading capacity of the SQ-PLGA NPs. SQ-PLGA NPs were characterized by NTA, FE-SEM, and UV-Vis spectroscopy and the ability to produce reactive oxygen species (ROS) was evaluated, proving that ROS generation ability is preserved in SQ-PLGA. In vitro antimicrobial activity against Gram-positive bacteria in planktonic state using Staphylococcus aureus was conducted in different conditions and pH to evaluate the potential of these nanophotosensitizers for aPDT in the local treatment of infections.