Person:
Olivares Olivares, Bernardo David

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First Name
Bernardo David
Last Name
Olivares Olivares
URI
https://hdl.handle.net/20.500.14352/76610
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Derecho
Department
Derecho Mercantil, Financiero y Tributario
Area
Derecho Financiero y Tributario
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2020 - 20233
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    Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress
    (Frontiers in Immunology, 2023) Wculek, Stefanie K.; Fernández-Bravo, Sergio; Torres-Ruiz, Raúl; Gomez-Sánchez, Maria José; Hernández-Walias, José Carlos; Moraes Ferreira, Frederico; Corraliza, Ana María; Sancho, David; Esteban, Vanesa; Rodriguez-Perales, Sandra; Nakaya, Helder I.; Salas, Azucena; Campos-Martín, Yolanda; Martínez-Zamorano, Elena; Muñoz-López, Diego; Blumberg, Richard S.; Martín Adrados, Beatriz; Valle Noguera, Ana; Cruz Adalia, Aranzazu; Olivares Olivares, Bernardo David; Gómez Del Moral Martín-Consuegra, Manuel María; Cubero Palero, Francisco Javier; Martínez Naves, Eduardo
    Introduction: The Unfolded Protein Response, a mechanism triggered by the cell in response to Endoplasmic reticulum stress, is linked to inflammatory responses. Our aim was to identify novel Unfolded Protein Response-mechanisms that might be involved in triggering or perpetuating the inflammatory response carried out by the Intestinal Epithelial Cells in the context of Inflammatory Bowel Disease. Methods: We analyzed the transcriptional profile of human Intestinal Epithelial Cell lines treated with an Endoplasmic Reticulum stress inducer (thapsigargin) and/or proinflammatory stimuli. Several genes were further analyzed in colonic biopsies from Ulcerative Colitis patients and healthy controls. Lastly, we generated Caco-2 cells lacking HMGCS2 by CRISPR Cas-9 and analyzed the functional implications of its absence in Intestinal Epithelial Cells. Results: Exposure to a TLR ligand after thapsigargin treatment resulted in a powerful synergistic modulation of gene expression, which led us to identify new genes and pathways that could be involved in inflammatory responses linked to the Unfolded Protein Response. Key differentially expressed genes in the array also exhibited transcriptional alterations in colonic biopsies from active Ulcerative Colitis patients, including NKG2D ligands and the enzyme HMGCS2. Moreover, functional studies showed altered metabolic responses and epithelial barrier integrity in HMGCS2 deficient cell lines. Conclusion: We have identified new genes and pathways that are regulated by the Unfolded Protein Response in the context of Inflammatory Bowel Disease including HMGCS2, a gene involved in the metabolism of Short Chain Fatty Acids that may have an important role in intestinal inflammation linked to Endoplasmic Reticulum stress and the resolution of the epithelial damage.
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    Programa de Derecho Financiero Tributario (I y II)
    (2020) Martínez Lago, Miguel Ángel; Almudí Cid, José Manuel; Calero García, María Luz; Gandarias Cebrián, Leopoldo; Olivares Olivares, Bernardo David; Sánchez-Archidona Hidalgo, Guillermo; Verdesoto Gómez, Marta
    Programa de las asignaturas impartidas en la Universidad Complutense de Madrid. Incluye orientaciones generales, fuentes básicas de conocimiento, las lecciones que integran el contenido de las asignaturas y horarios de tutorías para estudiantes durante el curso 2020-2021.
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    Project number: 313
    Estudio comparativo sobre el aprendizaje mediante la introducción gradual de recursos digitales y nuevas metodologías vs. el método de clase magistral
    (2020) Olivares Olivares, Bernardo David; Almudi Cid, José Manuel; Calero García, María Luz; Dimitrova Slavcheva, Slavka; Martínez Lago, Miguel Ángel; Verdesoto Gómez, Marta María
    La investigación tiene como objetivo encontrar evidencia científica que avale la eficiencia y eficacia de cada uno de los métodos propuestos para desarrollar las competencias del alumnado. Para ello, se crearán dos grupos docentes integrados por diversas asignaturas donde se emplearán de manera contrapuesta el método magistral y la docencia a través del empleo de recursos digitales y de autoaprendizaje (Flipped classroom, Open Educational Resources y M learning). Posteriormente se medirán y evaluarán los distintos aspectos relacionados con la percepción del alumnado y el desarrollo de las competencias en cada una de las materias.