Person:
Menéndez Ramos, José Carlos

First Name

José Carlos

Last Name

Menéndez Ramos

URI

https://hdl.handle.net/20.500.14352/77600

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Farmacia

Department

Química en Ciencias Farmacéuticas

Area

Química Orgánica

Identifiers

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Search Results

Now showing 1 - 10 of 10

Neuroprotective Action of Multitarget 7-Aminophenanthridin-6(5H)-one Derivatives against Metal-Induced Cell Death and Oxidative Stress in SN56 Cells
(ACS Chemical Neuroscience, 2021) Moyano-Cires Ivanoff, Paula Viviana; Vicente Zurdo, David; Blázquez-Barbadillo, Cristina; Menéndez Ramos, José Carlos; González Matilla, Juan Francisco; Rosales Conrado, Noelia; Pino Sans, Javier Del
Neurodegenerative diseases have been associated with brain metal accumulation, which produces oxidative stress (OS), matrix metalloproteinases (MMPs) induction, and neuronal cell death. Several metals have been reported to downregulate both the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway and the antioxidant enzymes regulated by it, mediating OS induction and neurodegeneration. Among a recently discovered family of multitarget 7-amino-phenanthridin-6-one derivatives (APH) the most promising compounds were tested against metal-induced cell death and OS in SN56 cells. These compounds, designed to have chelating activity, are known to inhibit some MMPs and to present antioxidant and neuroprotective effects against hydrogen peroxide treatment to SN56 neuronal cells. However, the mechanisms that mediate this protective effect are not fully understood. The obtained results show that compounds APH1, APH2, APH3, APH4, and APH5 were only able to chelate iron and copper ions among all metals studied and that APH3, APH4, and APH5 were also able to chelate mercury ion. However, none of them was able to chelate zinc, cadmium, and aluminum, thus exhibiting selective chelating activity that can be partly responsible for their neuroprotective action. Otherwise, our results indicate that their antioxidant effect is mediated through induction of the Nrf2 pathway that leads to overexpression of antioxidant enzymes. Finally, these compounds exhibited neuroprotective effects, reversing partially or completely the cytotoxic effects induced by the metals studied depending on the compound used. APH4 was the most effective and safe compound.
Project number: 377
Empleo de Software libre para la enseñanza en el área de la Química Orgánica
(2022) González Matilla, Juan Francisco; Gómez-Carpintero Jiménez, Jorge; Menéndez Ramos, José Carlos; Ramos García, María Teresa; Villacampa Sanz, Mercedes; Sanchez Cebrián, Juan Domingo
Twenty-first century antiepileptic drugs. An overview of their targets and synthetic approaches
(2024) Sánchez Cebrián, Juan Domingo; González Matilla, Juan Francisco; Menéndez Ramos, José Carlos
The therapeutic use of the traditional drugs against epilepsy has been hindered by their toxicity and low selectivity. These limitations have stimulated the design and development of new generations of antiepileptic drugs. This review explores the molecular targets and synthesis of the antiepileptic drugs that have entered the market in the 21st century, with a focus on manufacturer synthesis.
Mechanochemical Synthesis of Primary Amides
(The Journal of Organic Chemistry, 2021) Gómez-Carpintero Jiménez, Jorge; Sánchez Cebrián, J. Domingo; González Matilla, Juan Francisco; Menéndez Ramos, José Carlos
Ball milling of aromatic, heteroaromatic, vinylic, and aliphatic esters with ethanol and calcium nitride afforded the corresponding primary amides in a transformation that was compatible with a variety of functional groups and maintained the integrity of a stereocenter α to carbonyl. This methodology was applied to α-amino esters and N-BOC dipeptide esters and also to the synthesis of rufinamide, an antiepileptic drug.
Neuroprotective mechanisms of multitarget 7-aminophenanthridin-6(5H)-one derivatives against metal-induced amyloid proteins generation and aggregation
(Food and Chemical Toxicology, 2022) Moyano-Cires Ivanoff, Paula Viviana; Vicente Zurdo, David; Blázquez Barbadillo, Cristina; Menéndez Ramos, José Carlos; González Matilla, Juan Francisco; Rosales Conrado, Noelia; Pino Sans, Javier Del
Brain’s metals accumulation is associated with toxic proteins, like amyloid-proteins (Aβ), formation, accumulation, and aggregation, leading to neurodegeneration. Metals downregulate the correct folding, disaggregation, or degradation mechanisms of toxic proteins, as heat shock proteins (HSPs) and proteasome. The 7-amino-phenanthridin-6(5H)-one derivatives (APH) showed neuroprotective effects against metal-induced cell death through their antioxidant effect, independently of their chelating activity. However, additional neuroprotective mechanisms seem to be involved. We tested the most promising APH compounds (APH1-5, 10–100 μM) chemical ability to prevent metal-induced Aβ proteins aggregation; the APH1-5 effect on HSP70 and proteasome 20S (P20S) expression, the metals effect on Aβ formation and the involvement of HSP70 and P20S in the process, and the APH1-5 neuroprotective effects against Aβ proteins (1 μM) and metals in SN56 cells. Our results show that APH1-5 compounds chemically avoid metal-induced Aβ proteins aggregation and induce HSP70 and P20S expression. Additionally, iron and cadmium induced Aβ proteins formation through downregulation of HSP70 and P20S. Finally, APH1-5 compounds protected against Aβ proteins-induced neuronal cell death, reversing partially or completely this effect. These data may help to provide a new therapeutic approach against the neurotoxic effect induced by metals and other environmental pollutants, especially when mediated by toxic proteins.
Project number: 223
Virtualización de la docencia práctica. Visualización de montajes y funcionamiento de equipos de laboratorio para la mejora del aprendizaje a través de plataformas virtuales.
(2015) Giorgi Poletti, Giorgio; Sánchez Cebrián, Juan Domingo; Cledera Crespo, María del Pilar; Fernández Fernández, María; López-Alvarado Gutiérrez, María Pilar; Menéndez Ramos, José Carlos; González Matilla, Juan Francisco
Project number: PIMCD282/23-24
El podcast como herramienta de aprendizaje: Contribuciones de la Química Farmacéutica en el entorno “One Health”
(2024) Ruiz Serrano, Miriam; Menéndez Ramos, José Carlos; López-Alvarado Gutiérrez, María Pilar; Villacampa Sanz, Mercedes; González Matilla, Juan Francisco; Sánchez Cebrián, Juan Domingo; Sarabia Vallejo, Álvaro; Carmona Zafra, Noelia; Rodríguez Cordero, María Luisa; Herrero Gómez, Natalia; Solera Viana, María; Polo Parra, Sonia; Sánchez Cledera, Jaime; Ramos García, María Teresa
Este proyecto se presenta como continuación del proyecto de innovación docente Nº 96 (2022/2023) y tiene como objetivo crear un modelo didáctico basado en el aprendizaje autónomo y práctico del alumnado mediante la escucha, elaboración y/o edición de podcasts, abordando temas relacionados con la importancia de la Química Farmacéutica en el campo de la salud y el bienestar.
Project number: 143
Uso del vídeo tutorial como soporte multimedia para la comprensión de los mecanismos de las reacciones químicas en las competencias de la Química Orgánica
(2020) Cuesta Elósegui, Elena De La; Menéndez Ramos, José Carlos; López-Alvarado Gutiérrez, María Pilar; Ramos García, María Teresa; Villacampa Sanz, Mercedes; González Matilla, Juan Francisco; Cledera Crespo, María Pilar; Blázquez Barbadillo, Cristina; Sánchez Cebrián, Juan Domingo
Project number: 111
Desarrollo del aula virtual como soporte del aprendizaje flexible, de las competencias relacionadas con la determinación estructural de compuestos orgánicos
(2019) González Matilla, Juan Francisco; Cuesta Elósegui, Elena De La; Menéndez Ramos, José Carlos; Villacampa Sanz, Mercedes; Ramos García, María Teresa; Cores Esperón, Ángel
La mejora en la actividad docente y la eficacia del tiempo dedicado al aprendizaje por el alumno, están estrechamente ligadas al tiempo que dedica el alumno al aprendizaje fuera del aula. Con el desarrollo y avance de las TIC, en los últimos tiempos ha ganado terreno una práctica de aprendizaje que tiene su origen en la educación a distancia y que se conoce hoy como e-learning, educación virtual o, educación en línea. En este sentido hemos diseñado y elaborado una serie de fichas de problemas sobre determinación estructural de compuestos orgánicos. En este proyecto también se han elaborado vídeos tutoriales donde se indican las pautas necesarias para resolver los problemas de determinación estructural.
Project number: 263
Aprendizaje de las prácticas de laboratorio de Química Farmacéutica del Grado en Farmacia y Doble Grado en Farmacia y Nutrición a través del aula virtual
(2022) Menéndez Ramos, José Carlos; Hernáiz Gómez-Degano, María Josefa; Pedregal Freire, Carmen; Cabezas Baudot, Nieves; Villacampa Sanz, Mercedes; Campo Pérez, Carmen Luisa Del; Salazar Martínez De Pisón, María Loreto; González Matilla, Juan Francisco; Hoyos Vidal, María Pilar; Esteban García, Gemma; Martín Martín, Antonio; Rico Arjona, Rocío; Gómez-Carpintero Jiménez, Jorge; Martín Cámara, Olmo; Cores Esperón, Ángel