Person: Sevillano Fernández, David
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First Name
David
Last Name
Sevillano Fernández
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Medicina
Area
Microbiología
Identifiers
19 results
Search Results
Now showing 1 - 10 of 19
- Project number: PIMCD50/23-24
Item Descubriendo la microbiota y su relación con los portadores nasales de S. aureus: un proyecto innovador de enseñanza para la formación en salud(2024) González Hidalgo, Natalia; Gómez-Lus Centelles, María Luisa; Alou Cervera, Luis; Sevillano Fernández, David; Bas Caro, Pedro; Gambín Güemes, Aixa Bárbara Item Strengthening the relationship between intractable plantar keratosis and human papillomavirus(Journal of Medical Virology, 2024) Alou Cervera, Luis; Becerro De Bengoa Vallejo, Ricardo; Losa Iglesias, Marta Elena; Moreno, Juan; Sánchez Gómez, Rubén; González Hidalgo, Natalia; Sevillano Fernández, DavidThe aim of the study was to determine the presence of human papillomavirus (HPV) in patients with intractable plantar keratosis (IPK) by comparing the histopathological findings of biopsies. A prospective, observational, and concordance study was carried out. Three different specimens were taken from each IPK. A first punch was sent for histopathological examination, and a second punch and a superficial skin scraping were both sent for HPV polymerase chain reaction (PCR) and type determination. A total of 51 patients were included. From the histopathological examination, it was determined that 35 (68.6%) samples were diagnosed as warts and 16 (31.3%) as keratosis. However, the presence of HPV was confirmed by PCR in 49 (96.1%) and in 42 (82.4%) samples obtained by punch and superficial scraping, respectively. In the 49 PCR‐positive samples, the most common HPV types were HPV1, HPV2, HPV27, HPV57, and HPV65, accounting for 81.6% of the samples. In conclusion, this study demonstrates that HPV infection and IPK lesions are very closely related. Although we cannot confirm that HPV is the cause of the development of IPK, the high prevalence of HPV observed in these lesions calls for a change to the procedures for managing IPK.Item A deeper look at low‐frequency contact ultrasonic debridement in the clinical management of patients with diabetic foot ulcers(Wound Repair and Regeneration: The International Journal of Tissue Repair and Regeneration, 2023) Álvaro Afonso, Francisco Javier; Flores Escobar, Sebastián; Sevillano Fernández, David; García Álvarez, Yolanda; García-Madrid Martín De Almagro, Marta; Lázaro Martínez, José LuisThe current study aimed to evaluate the dispersal of solution and microbes (aerosol) in the clinical environment during treatment with Low-frequency contact ultrasonic debridement (LFCUD) with or without suction attachment in patients with diabetic foot ulcers (DFUs). We performed 20 treatments in 10 patients divided into two groups to receive the proposed LFCUD modalities. We measured the microbial load of the environment pre-treatment (sample M1), during treatment with each LFCUD modality (sample M2) and post-treatment (sample M3). The use of LFCUD debridement without a suction attachment results in significantly higher immediate contamination of the clinic environment than the suction attachment, particularly during the procedure (1.70 ± 0.98 log 10 CFU/mL versus 0.77 ± 0.85 log 10 CFU/mL, p = 0.035). When suction is not applied, there are statistically significant differences depending on whether the DFUs are neuropathic or neuroischemic, finding a greater number of microorganisms with high loads in neuropathic DFUs. We found a statistically significant positive correlation between wound area (r = 0.450, p = 0.047) and TBI (r = 0.651, p = 0.006) with the bacterial load during the LFCUD. Based on our results, we recommend using the personal protective equipment required to protect staff members and patients during treatment with LFCUD and using a suction attachment where clinically possible to reduce clinic environmental pollution, especially in neuropathic DFUs and those with larger areas.Item Alcohol-Based Chlorhexidine and Potassium Sorbate Rub Strengthens the Effectiveness of Traditional Hand Scrubbing and Improves Long-Lasting Effectiveness—Evaluation of Hand Preparation Protocols According to EN 12791(Antibiotics, 2024) Herráiz Soria, Elena; Alou Cervera, Luis; Martin Villa, Carlos; Becerro De Bengoa Vallejo, Ricardo; Losa Iglesias, Marta; Sevillano Fernández, David; Simões, ManuelDespite the advantages of surgical handrub in terms of the ease of application and effectiveness, chlorhexidine (CHG)-based hand scrubbing remains the preferred method for surgical hand preparation. However, it does not systematically meet the non-inferiority requirement of the European norm (EN) 12791 with respect to n-propanol (the reference product) and does not provide the sustained efficacy expected for these long-lasting agents. Commercially available alcohol-based products have also failed to demonstrate sustained efficacy according to EN 12791. Multi-step protocols enhance the efficacy of hand scrubbing, yet their extended disinfection duration might diminish their allure for healthcare professionals. In this study, we show that hand scrubbing with CHG 4% followed by a 1 min rubbing with the novel formulation of ethanol (Et) 70%/CHG 3% plus 0.3% potassium sorbate food additive (PS) meets the non-inferiority requirement and demonstrates sustained efficacy when tested according to EN 12791. The immediate and 3 h effect of this protocol was significantly higher than that of n-propanol and the homologous disinfection protocol without PS (CHG 4% hand scrub plus Et 70%/CHG 3% rub), demonstrating that the inclusion of PS confers a notable residual effect. We speculate that this non-volatile ingredient acts synergistically with CHG. This promising combination represents an alternative method for the development of new disinfection strategies.Item Do face masks increase the rate of the Staphylococcus aureus nasal carriers?(Revista Española de Quimioterapia, 2023) Urquía Cutillas, Salvador; Gómez-Lus Centelles, María Luisa; Sevillano Fernández, David; González Hidalgo, Natalia; Alou Cervera, Luis; Barberán, JoséItem 3Y-TZP/Ta Biocermet as a Dental Material: An Analysis of the In Vitro Adherence of Streptococcus Oralis Biofilm and an In Vivo Pilot Study in Dogs(Antibiotics, 2024) Smirnov, Anton; Yanushevich, Oleg; Krikheli, Natella; Solis Pinargote, Nestor Washington; Peretyagin, Pavel; Grigoriev, Sergey; Alou Cervera, Luis; Sevillano Fernández, David; López Piriz, Roberto; Guitian, Francisco; Bartolomé Gómez, José Florindo; Varoni, ElenaThe surface adhesion of bacterial cells and the in vivo biocompatibility of a new ceramic–metal composite made of zirconium dioxide and tantalum were evaluated. Within the framework of an in vitro study using the crystal violet staining and colony counting methods, a relatively similar adhesion of Streptococcus oralis to the 3Y-TZP/Ta biocermet (roughness Ra = 0.12 ± 0.04 µm) and Ti-Al6-V4 titanium alloy (Ra = 0.04 ± 0.01 µm) was found. In addition, in an in vivo preliminary study focused on the histological analysis of a series of rods implanted in the jaws of beagle dogs for a six-month period, the absence of any fibrous tissue or inflammatory reaction at the interface between the implanted 3Y-TZP/Ta biocermets and the new bone was found. Thus, it can be concluded that the developed ceramic–metal biocomposite may be a promising new material for use in dentistry.- Project number: 177
Item Elaboración de material docente virtual para las prácticas de la asignatura de Microbiología del Grado de Podología(2021) Alou Cervera, Luis; Sevillano Fernández, David; González Hidalgo, Natalia; Bas Caro, Pedro; Domínguez Guardiola, Natalia; Álvaro Afonso, Francisco Javier; Molines Barroso, Raúl Juan; Lázaro Martínez, José LuisEl aprendizaje mixto con la incorporación de nuevas tecnologías como vídeos cortos y cuestionarios online ha permitido mejorar el rendimiento, la satisfacción y el compromiso de los estudiantes. El objetivo de este Proyecto de Innovación Docente fue elaborar material docente virtual para las prácticas de la asignatura de Microbiología del Grado de Podología. Al inicio del curso se elaboró un cuestionario para valorar los conocimientos previos en materia de microbiología del alumnado. Posteriormente los alumnos se inscribieron de forma voluntaria a las prácticas de Microbiología eligiendo entre el formato virtual o presencial. El guion teórico de prácticas presenciales se adaptó a un formato virtual incorporando multitud de imágenes y ejemplos prácticos de las pruebas de diagnóstico microbiológico y centrados en dos áreas prioritarias de diagnóstico, las infecciones de pie diabético y las infecciones fúngicas en podología. Posteriormente, realizaron dos casos clínicos prácticos tanto en las prácticas presenciales como en las virtuales. Estos últimos debieron contestar un cuestionario sobre los casos prácticos así como valorar la actividad. Por último, los alumnos respondieron de nuevo al cuestionario de conocimientos planteado al inicio del curso académico. Todo el material fue subido al Campus Virtual para su consulta y resolución. De los 71 alumnos matriculados, 19 (26,8%) eligieron realizar las prácticas en formato virtual y 52 (73,2%) en formato presencial. Tanto las prácticas en formato virtual como presencial mejoraron el rendimiento de los alumnos, observándose un incremento significativo de 1,9 y de 2,0 en la calificación para el grupo de prácticas en formato virtual y presencial, respectivamente. No se observaron diferencias significativas entre ambos formatos (virtual y presencial) en la calificación obtenida después de realizar las prácticas. Se observó un nivel alto de participación de la actividad. Un 71,8% de los alumnos realizaron el cuestionario previo de conocimientos y un 66,2% realizaron el cuestionario de conocimientos después de las prácticas. La participación del alumno que eligió el formato presencial fue superior a la del alumno que eligió el formato presencial. El grado de satisfacción de las prácticas en formato virtual fue elevado ya que se obtuvo un valor medio de 4 sobre 5. Item Influence of Sexual Dimorphism, Aging, and Differential Cell Capture Efficiency of Blood Separation Systems on the Quality of Platelet-Rich Plasma(Journal of Clinical Medicine, 2022) Trevissón, Bibiana; Becerro de Bengoa Vallejo, Ricardo; Sevillano Fernández, David; González, Natalia; Losa Iglesias, Marta Elena; López López, Daniel; Alou Cervera, LuisFew studies have checked the impact of the hormonal/immunological dimorphism of patients on the cellular composition of platelet-rich plasma products (PRP). Whole blood (WB) from 26 volunteers was concentrated using a device previously characterized. Platelet and white blood cell (WBC) counts in WB and PRP were compared between genders, and after the population was split into pre (≤50 years)- and post (>50 years)-menopausal ages. In WB, platelet–total WBC densities were comparable in men and women. The phagocytic cell composition differed (p ≤ 0.04). After dividing by ages, phagocytic cell discrepancies were linked to women > 50 years (p ≤ 0.05), and differences emerged in lymphocyte counts (lower in >50 years groups, within and between genders, p ≤ 0.05). In PRP, densities were significantly higher, but the PRP/WB ratios varied according to blood cell (lower for phagocytic cells) and between subjects (more favorable at a lower density of a particular blood cell in WB). This “system compensatory efficiency” reduced/reverted PRP differences in the leukocyte composition between genders/age–sex groups in WB. In PRP, neutrophils were higher in younger men than older women (p < 0.05). WB lymphocyte differences between age–sex groups persisted. Age is a more determining factor than sex in the preparation of PRP. Post-menopause, sexual dimorphism strongly influences the composition of leukocytes, also conditioned by the capture efficiency of the system.Item Innovative Medial Cushioning Orthoses Affect Peroneus Longus Electromyographic Activity during Running(Journal of Clinical Medicine, 2022) Sánchez Gómez, Rubén; Gómez Carrión, Álvaro; Martinez Sebastian, Carlos; Alou Cervera, Luis; Sevillano Fernández, David; Nuñez Fernández, Almudena; Sanz Wozniak, Paola; De la Cruz Torres, BlancaBackground: Over-supination processes of the foot and ankle involving peroneus longus (PL) damage during running sports have been treated conservatively with passive control tools, such as tapes, braces, or external ankle supports, but the effect of orthoses with typical lateral wedging orthoses (TLWO) on the muscular activity of PL during running remains unclear. Here we investigate the effects of innovative medial cushioning orthoses (IMCO) on PL activity during the full running gait cycle. In addition, we wished to ascertain the effects of innovative medial cushioning orthoses (IMCO) on PL activity during running. Methods: Thirty-one healthy recreational runners (mean age 34.5 ± 3.33) with neutral foot posture index scores, were selected to participate in the present study. They ran on a treadmill at 9 km/h wearing seven different orthoses (NRS, IMCO 3 mm, IMCO 6 mm, IMCO 9 mm, TLWO 3 mm, TLWO 6 mm and TLWO 9 mm), randomly performed on the same day while electromyographic activity of the PL muscle was recorded. Statistical intraclass correlation coefficient (ICC) to test reliability was carried out and the Wilcoxon test with Bonferroni’s correction was developed to analyze the differences between the conditions. Results: the reliability of all assessments showed data higher than 0.81, that is, “almost perfect reliability”; all EMG PL values wearing either TLWO or IMCO showed a statistically significant reduction versus NRS during the fully analyzed running gait cycle; the highest difference was set on NRS 23.08 ± 6.67 to TLWO 9 mm 17.77 ± 4.794 (p < 0.001). Conclusions: Muscular EMG activity of the PL during the full running gait cycle decreases when wearing either TLWO or IMCO relative to NRS; therefore, these orthoses could be prescribed to treat the strain and overload pathologies of PL. In addition, IMCO—as it less thick, compared with TLWO—can be used when aiming to achieve better running economy.Item Seconeolitsine, the Novel Inhibitor of DNA Topoisomerase I, Protects against Invasive Pneumococcal Disease Caused by Fluoroquinolone-Resistant Strains(Antibiotics, 2021) Tirado Vélez, Jose Manuel; Carreño, David; Sevillano Fernández, David; Alou Cervera, Luis; Yuste, José; de la Campa, Adela G.Antibiotic resistance in Streptococcus pneumoniae has increased worldwide, making fluoroquinolones an alternative therapeutic option. Fluoroquinolones inhibit the type II DNA topoisomerases (topoisomerase IV and gyrase). In this study we have evaluated the in vivo activity of seconeolitsine, an inhibitor of topoisomerase I. Levofloxacin (12.5 to 50 mg/kg) or seconeolitsine (5 to 40 mg/kg) were administered every 12 h during two days in mice infected with a serotype 8-resistant strain. At 48 h, a 70% protection was obtained with seconeolitsine (40 mg/kg; p < 0.001). However, survival with levofloxacin was 20%, regardless of the dose. In addition, seconeolitsine decreased bacteremia efficiently. Levofloxacin had higher levels in serum than seconeolitsine (Cmax of 14.7 vs. 1.6; p < 0.01) and higher values of area under the serum concentration-time curve (AUC0-12h of 17.3 vs. 5; p < 0.01). However, seconeolitsine showed higher levels of time to peak concentration and elimination half-life. This is consistent with the higher binding of seconeolitsine to plasma proteins (40% and 80% when used at 1 µg/mL and 50 µg/mL, respectively) in comparison to levofloxacin (12% at 5 µg/mL and 33% at 50 µg/mL). Our results suggest that seconeolitsine would be a promising therapeutic alternative against pneumococcal isolates with high fluoroquinolone resistance levels.