Person: Sevillano Fernández, David
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First Name
David
Last Name
Sevillano Fernández
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Medicina
Area
Microbiología
Identifiers
7 results
Search Results
Now showing 1 - 7 of 7
Item Comparing rubbing and scrubbing surgical hand antisepsis with propan-1-ol 60% in accordance with European regulation UNE-EN 12791:2016+A1:2018(Infection Control & Hospital Epidemiology, 2021) Martin Villa, Carlos; Becerro De Bengoa Vallejo, Ricardo; Alou Cervera, Luis; González Hidalgo, Natalia; Losa Iglesias, Marta; Gómez-Lus Centelles, María Luisa; Sevillano Fernández, David; Cambridge University PressA crossover trial was conducted to compare hand rub and hand scrub-brush methods for reducing bacterial loads when using propan-1-ol-60% according to European regulations. Both methods significantly reduced the bacterial load immediately after antisepsis, but only the hand rub method achieved significant bacterial load reduction 3 hours after the procedure.Item Effect on the reduction of bacterial load after surgical hand antisepsis with triclosan 0.5% compared to triclosan 0.5% followed by 70% alcoholic solution(Infection Control & Hospital Epidemiology, 2021) Santé Serna, Luis Narciso; Gómez-Lus Centelles, María Luisa; Martin Villa, Carlos; Becerro De Bengoa Vallejo, Ricardo; Alou Cervera, Luis; Sevillano Fernández, DavidTriclosan 0.5% by scrubbing does not meet the UNE-EN12791 criteria to be used in the surgical hand preparation (SHP). Triclosan 0.5% by scrubbing followed by ethanol 70% hand rubbing is suitable without the additional characteristic of sustained effect. This limited effectiveness implies that triclosan should be avoided in SHP given the restrictions on its use in consumer antiseptic products. The trial was registered at ClinicalTrials.gov (ID: NCT04538365).Item Estudiando la microbiota a través de la prevalencia de portadores nasales de Staphylococcus aureus en los estudiantes de Microbiología del Grado de Podología(European Journal of Podiatry, 2020) Gómez-Lus Centelles, María Luisa; Ciudad Cabañas, María José; Collado Yurrita, Luis Rodolfo; Sevillano Fernández, David; González Hidalgo, Natalia; Alou Cervera, LuisObjetivos. Se ha planteado como objetivo la mejora de la calidad de la docencia de la Microbiología mediante la actualización de la metodología docente, introduciendo como actividad el aprendizaje activo basado en la detección de portadores nasales de Staphylococcus aureus para conseguir mejorar las competencias que deberán adquirir los estudiantes como parte de su formación integral. Material y métodos. En este estudio han participado 56 y 72 alumnos (128 alumnos en total) del Grado de Podología de la Universidad Complutense de Madrid de los cursos 2018-2019 y 2019-2020, respectivamente. Los alumnos asistieron al laboratorio de Microbiología y completaron el estudio. Resultados. Un total de 29 alumnos (22,7%) fueron portadores nasales de S. aureus, uno de los cuales fue portador de S. aureus resistente a meticilina (SARM). La valoración de la actividad por parte de los alumnos fue muy positiva y se produjo una mejora general en la adquisición de conocimientos. Conclusión. El trabajo en el laboratorio de Microbiología unido a la actualización en la metodología docente puede mejorar el rendimiento académico de los estudiantes de Microbiología al relacionarlo con su futura práctica profesional y el diagnóstico microbiológico.Item Fungal bioaerosol as an occupational hazard in the podiatrist’s workplace(International Journal of Environmental Health Research, 2021) Alija Martínez, Beatriz; Becerro De Bengoa Vallejo, Ricardo; Sevillano Fernández, David; González Hidalgo, Natalia; Losa Iglesias, Marta Elena; Collado Yurrita, Luis Rodolfo; Espinosa Rubio, Rubén; Alou Cervera, LuisPrevious studies have reported the potential emission of nail dust to the indoor air of the podiatrist's workplace during nail drilling procedures. The main objective of this study was to analyse the fungal bioaerosol present in the indoor air of podiatry rooms, including during the nail drilling procedure. Seventeen private clinics were sampled in Madrid during spring and summer. The most prevalent filamentous fungi detected were Cladosporium spp. and Penicillium spp. in both seasons. The presence of Trichophyton rubrum and Trichophyton interdigitale was reported in samples during the drilling procedures, establishing a positive association with the clinical suspicion of onychomycosis (p = 0.002) and the utilization of aspiration systems (p=0.04). The present study concludes that airborne fungal concentrations in podiatrist's clinics are consistent with national and international guidance. Occupational exposure to dermatophytes may briefly occur during definite periods across the daily activity despite the use of dust aspiration systems.Item Bactericidal ZnO glass-filled thermoplastic polyurethane and polydimethyl siloxane composites to inhibit biofilm-associated infections(Scientific Reports, 2019) Cabal, Belén; Sevillano Fernández, David; Fernández García, Elisa; Alou Cervera, Luis; Suárez, Marta; González Hidalgo, Natalia; Moya, José S.; Torrecillas, RamónThis study investigates a novel approach to controlling biofilms of the most frequent pathogens implicated in the etiology of biomaterials-associated infections. New bactericidal filler based on a non-toxic glass, belonging to B2O3-SiO2-Al2O3-Na2O-ZnO system, was used to formulate composites of the most widely used polymers in biomedical applications [i.e. thermoplastic polyurethane (TPU) and polydimethyl siloxane (PDMS)], with varying percentage by weight of the bactericidal glass (5, 15, 25, 35, 50%). Glass-filled polymer composites show dramatically restricted bacterial colonisation and biofilm formation. They exhibit time- and dose-dependent killing, with maximal action at 5 days. The highest activity was found against S.epidermidis biofilm (99% of reduction), one of the most common cause of nosocomial infections. The tensile properties of the obtained glass-filled composites are comparable with the literature data concerning polymeric biomaterials for medical implants and devices. In addition, all the materials presented in this research, revealed an excellent biocompatibility. This was disclosed by cell viability values above 70%, none alteration on erythrocyte membrane or cell functionality in contact with materials (haemolytic index 0–2%), and absence of interferences in blood coagulation (intrinsic, extrinsic and final pathways).Item Prevalence of Staphylococcus spp. nasal colonization among doctors of podiatric medicine and associated risk factors in Spain(Antimicrobial Resistance & Infection Control, 2018) Benito, Sheila de; Alou Cervera, Luis; Becerro De Bengoa Vallejo, Ricardo; Losa Iglesias, Marta Elena; Gómez-Lus Centelles, María Luisa; Collado Yurrita, Luis Rodolfo; Sevillano Fernández, DavidBackground: This study aimed to estimate the prevalence of methicillin-susceptible and -resistant Staphylococcus aureus (MSSA and MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE) nasopharyngeal carriage among Doctors of Podiatric Medicine (Podiatrists) and to determine the potential risk factors. Methods: A cross-sectional study was carried out in 2016-2017 among 239 podiatrists in Spain. The presence of MSSA, MRSA, and MRSE was determined by microbiological analysis of nasal exudate and antimicrobial susceptibility was determined. Each podiatrist completed a questionnaire. The questionnaire comprised various parameters such as sex, age, podiatry experience duration, underlying diseases, prior antibiotic treatment, hospitalization during the last year, and use of a protective mask, an aspiration system, or gloves. Results: The prevalence of MSSA, MRSA, and MRSE was 23.0%, 1.3%, and 23.8%, respectively. The MSSA prevalence was higher among podiatrists who did not use an aspiration system (32.3%) compared to those who did (19.3%; p = 0.0305), and among podiatrists with respiratory diseases (36.8%) compared to those without (20.8%; p = 0.0272). The MRSE prevalence was higher among men (33.7%) compared to women (8.6%; p = 0.0089), podiatrists aged ≥50 (38.5%) compared to ≤35 (17.8%; p = 0.0101), and podiatrists with ≥15 (39.3%) compared to ≤5 years of podiatry experience (12.5%; p = 0.0015). Among the S. aureus strains, 84.5% were resistant to penicillin, 22.4% to erythromycin, 20.7% to clindamycin, and 12.7% to mupirocin. The MRSE strains were resistant to penicillin (93.0%), erythromycin (78.9%), and mupirocin (73.7%). Conclusions: The prevalence of S. aureus and S. epidermidis nasal carriage is low among Spanish podiatrists compared to other health professionals.Item Evaluating the optimal time for amikacin administration with respect to haemodialysis using an in vitro pharmacodynamic simulation against epidemic nosocomial OXA-48 producing Klebsiella pneumoniae ST405 strains(Journal of Global Antimicrobial Resistance, 2019) Carcas, Antonio J.; Sevillano Fernández, David; González Hidalgo, Natalia; Alou Cervera, Luis; Gómez Gil, Rosa; Muñoz, Mario; Llanos, Lucía; Sánchez Villanueva, Rafael J.; Gonzalez Parra, Emilio; Giménez, María José; Aguilar, LorenzoObjectives: Bacterial viability and enrichment of resistance resulting from three different amikacin administration schedules with respect to haemodialysis (HD) were assessed against three OXA-48-producing Klebsiella pneumoniae isolated during an outbreak in a Spanish hospital. Methods: A previously described two-compartment dynamic system was used. Three possible amikacin administration schedules were simulated: (i) haemodialysis immediately after amikacin infusion (pre-HD); (ii) infusion immediately after haemodialysis (post-HD); and (iii) infusion at 50% interdialytic period. Amikacin standard dose (SD) and double dose (DD) were simulated for each schedule. Values of Cmax/MIC, Cmax/MPC (mutant prevention concentration), AUC0-48h/MIC, AUC0-48h/MPC and %TMSW (percentage of time that the concentration was inside the mutant selection window) were determined with experimental data and were correlated with the area under the bacterial killing curve of the total population and the resistant subpopulation. Results: Both with SD and DD, the pre-HD schedule resulted in increases at 48h in bacterial counts of the total population at the expense of enrichment of pre-existing resistant subpopulations from 12h onwards for all strains. The estimated %TMSW that prevented enrichment of resistant mutants was <61.5%. The AUC0-48h/MPC (with values of ≈40 being associated with countering of increases in resistant subpopulations) was better than the %TMSW as a predictive parameter. Conclusion: This study showed that the longest times concentrations were above the MPC (i.e. highest AUC0-48h/MPC, lowest %TMSW), the lowest enrichment of resistant subpopulations. This implies use of the highest possible amikacin dose (limited by toxicity) and post-HD as the best administration schedule.