Person:
Sevillano Fernández, David

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First Name
David
Last Name
Sevillano Fernández
URI
https://hdl.handle.net/20.500.14352/79192
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Medicina
Area
Microbiología
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2023 - 20243
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Subject: 579.2 ×

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    Alcohol-Based Chlorhexidine and Potassium Sorbate Rub Strengthens the Effectiveness of Traditional Hand Scrubbing and Improves Long-Lasting Effectiveness—Evaluation of Hand Preparation Protocols According to EN 12791
    (Antibiotics, 2024) Herráiz Soria, Elena; Alou Cervera, Luis; Martin Villa, Carlos; Becerro De Bengoa Vallejo, Ricardo; Losa Iglesias, Marta; Sevillano Fernández, David; Simões, Manuel
    Despite the advantages of surgical handrub in terms of the ease of application and effectiveness, chlorhexidine (CHG)-based hand scrubbing remains the preferred method for surgical hand preparation. However, it does not systematically meet the non-inferiority requirement of the European norm (EN) 12791 with respect to n-propanol (the reference product) and does not provide the sustained efficacy expected for these long-lasting agents. Commercially available alcohol-based products have also failed to demonstrate sustained efficacy according to EN 12791. Multi-step protocols enhance the efficacy of hand scrubbing, yet their extended disinfection duration might diminish their allure for healthcare professionals. In this study, we show that hand scrubbing with CHG 4% followed by a 1 min rubbing with the novel formulation of ethanol (Et) 70%/CHG 3% plus 0.3% potassium sorbate food additive (PS) meets the non-inferiority requirement and demonstrates sustained efficacy when tested according to EN 12791. The immediate and 3 h effect of this protocol was significantly higher than that of n-propanol and the homologous disinfection protocol without PS (CHG 4% hand scrub plus Et 70%/CHG 3% rub), demonstrating that the inclusion of PS confers a notable residual effect. We speculate that this non-volatile ingredient acts synergistically with CHG. This promising combination represents an alternative method for the development of new disinfection strategies.
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    Do face masks increase the rate of the Staphylococcus aureus nasal carriers?
    (Revista Española de Quimioterapia, 2023) Urquía Cutillas, Salvador; Gómez-Lus Centelles, María Luisa; Sevillano Fernández, David; González Hidalgo, Natalia; Alou Cervera, Luis; Barberán, José
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    3Y-TZP/Ta Biocermet as a Dental Material: An Analysis of the In Vitro Adherence of Streptococcus Oralis Biofilm and an In Vivo Pilot Study in Dogs
    (Antibiotics, 2024) Smirnov, Anton; Yanushevich, Oleg; Krikheli, Natella; Solis Pinargote, Nestor Washington; Peretyagin, Pavel; Grigoriev, Sergey; Alou Cervera, Luis; Sevillano Fernández, David; López Piriz, Roberto; Guitian, Francisco; Bartolomé Gómez, José Florindo; Varoni, Elena
    The surface adhesion of bacterial cells and the in vivo biocompatibility of a new ceramic–metal composite made of zirconium dioxide and tantalum were evaluated. Within the framework of an in vitro study using the crystal violet staining and colony counting methods, a relatively similar adhesion of Streptococcus oralis to the 3Y-TZP/Ta biocermet (roughness Ra = 0.12 ± 0.04 µm) and Ti-Al6-V4 titanium alloy (Ra = 0.04 ± 0.01 µm) was found. In addition, in an in vivo preliminary study focused on the histological analysis of a series of rods implanted in the jaws of beagle dogs for a six-month period, the absence of any fibrous tissue or inflammatory reaction at the interface between the implanted 3Y-TZP/Ta biocermets and the new bone was found. Thus, it can be concluded that the developed ceramic–metal biocomposite may be a promising new material for use in dentistry.