Person:
Andrés Gamazo, Paloma Jimena De

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First Name
Paloma Jimena De
Last Name
Andrés Gamazo
URI
https://hdl.handle.net/20.500.14352/80475
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Medicina y Cirugía Animal
Area
Medicina y Cirugía Animal
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Now showing 1 - 7 of 7
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    Establishment and Characterization of a New Cell Line of Canine Inflammatory Mammary Cancer: IPC-366
    (PLoS ONE, 2015) Cáceres Ramos, Sara Cristina; Peña Fernández, Laura Luisa; Andrés Gamazo, Paloma Jimena De; Illera Del Portal, Josefina María; Lopez, Mirtha S.; Woodward, Wendy A.; Reuben, James M.; Illera Del Portal, Juan Carlos
    Canine inflammatory mammary cancer (IMC) shares epidemiologic, histopathological and clinical characteristics with the disease in humans and has been proposed as a natural model for human inflammatory breast cancer (IBC). The aim of this study was to characterize a new cell line from IMC (IPC-366) for the comparative study of both IMC and IBC. Tumors cells from a female dog with clinical IMC were collected. The cells were grown under adherent conditions. The growth, cytological, ultrastructural and immunohistochemical (IHC) characteristics of IPC-366 were evaluated. Ten female Balb/SCID mice were inoculated with IPC-366 cells to assess their tumorigenicity and metastatic potential. Chromosome aberration test and Karyotype revealed the presence of structural aberration, numerical and neutral rearrangements, demonstrating a chromosomal instability. Microscopic examination of tumor revealed an epithelial morphology with marked anysocytosis. Cytological and histological examination of smears and ultrathin sections by electron microscopy revealed that IPC-366 is formed by highly malignant large round or polygonal cells characterized by marked atypia and prominent nucleoli and frequent multinucleated cells. Some cells had cytoplasmic empty spaces covered by cytoplasmic membrane resembling capillary endothelial cells, a phenomenon that has been related to s vasculogenic mimicry. IHC characterization of IPC-366 was basal-like: epithelial cells (AE1/AE3+, CK14+, vimentin+, actin-, p63-, ER-, PR-, HER-2, E-cadherin, overexpressed COX-2 and high Ki-67 proliferation index (87.15 %). At 2 weeks after inoculating the IPC-366 cells, a tumor mass was found in 100 % of mice. At 4 weeks metastases in lung and lymph nodes were found. Xenograph tumors maintained the original IHC characteristics of the female dog tumor. In summary, the cell line IPC-366 is a fast growing malignant triple negative cell line model of inflammatory mammary carcinoma that can be used for the comparative study of both IMC and IBC.
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    Project number: PIMCD155/23-24
    Elaboración de un atlas fotográfico en formato digital y físico de órganos sanos como complemento a la docencia de Anatomía Patológica Especial y Rotatorio Clínico
    (2024) Andrés Gamazo, Paloma Jimena De; García Fernández, Rosa Ana; Jiménez Martínez, María De Los Ángeles; García Palencia, María Del Pilar; Sánchez Maldonado, María Belén; Peña Fernández, Laura Luisa; González Huecas, Marta; Tabanera De Lucio, Enrique; Barreno San Antolín, Lucía; Valdivia Lara, Edgar Guillermo; Sánchez Pérez, María De Los Ángeles; Andrés Gamazo, Paloma Jimena De
    El proyecto se centró en crear un atlas fotográfico digital y físico de órganos sanos para estudiantes de Veterinaria, con el objetivo de mejorar la identificación de lesiones durante las necropsias y fomentar el aprendizaje autónomo. A raíz de la pandemia de COVID-19, se incorporaron tecnologías digitales en la docencia, lo que subrayó la necesidad de herramientas didácticas innovadoras en el Grado en Veterinaria, que tiene una elevada carga práctica. Para realizar el atlas se recopilaron y clasificaron imágenes de órganos sanos de diferentes fuentes, se creó un espacio en el Campus Virtual para mostrar el atlas en su versión digital, y paralelamente las imágenes se imprimieron y plastificaron para su uso en la sala de necropsias. Finalmente, se realizó una evaluación de la eficacia del atlas en ambos formatos mediante encuestas anónimas a los estudiantes. Los resultados preliminares fueron difundidos internacionalmente en el VII Congreso Español y I Hispano-Portugués de Docencia Veterinaria organizado por la Asociación Española de Docentes Veterinarios (VetDoc). Los resúmenes de los trabajos, además de en el libro de resúmenes del congreso, serán publicados en la revista Frontiers in Veterinary Science. La recopilación de imágenes resultó desafiante, pero se logró recopilar una suficiente cantidad de imágenes de las especies canina, felina, equina, porcina, bovina y de pequeños rumiantes. La implementación en el Campus Virtual facilitó el acceso continuo al recurso, mientras que la versión física fue esencial para las prácticas en la sala de necropsias. La evaluación del proyecto, a través de encuestas anónimas, mostró que los estudiantes apreciaron y utilizaron el atlas en ambos formatos, destacando su utilidad para otras asignaturas y su efectividad en mejorar el proceso de enseñanza y aprendizaje. La preferencia general fue por el formato digital, aunque el físico también fue muy valorado en las prácticas. En conclusión, el proyecto no solo mejoró la enseñanza de Anatomía Patológica en Veterinaria, sino que también demostró la validez de integrar tecnologías digitales en la educación práctica. La metodología y los resultados pueden servir como referencia para futuros proyectos educativos, contribuyendo al avance de la educación veterinaria.
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    Canine cell line, IPC‐366, as a good model for the study of inflammatory breast cancer
    (Veterinary and Comparative Oncology, 2016) Cáceres Ramos, Sara Cristina; Peña Fernández, Laura Luisa; Lacerda, Lara; Illera Del Portal, Josefina María; Andrés Gamazo, Paloma Jimena De; Larson, Richard; Gao, Hui; Debeb, Bisrat; Woodward, Wendy; Reuben, James; Illera Del Portal, Juan Carlos
    Inflammatory breast cancer (IBC) is an aggressive type of cancer with poor survival in women. Inflammatory mammary cancer (IMC) in dogs is very similar to human IBC and it has been proposed as a good surrogate model for study the human disease. The aim was to determine if IPC-366 shared characteristics with the IBC cell line SUM149. The comparison was conducted in terms of ability to grow (adherent and nonadherent conditions), stem cell markers expression using flow cytometry, protein production using western blot and tumorigenic capacity. Our results revealed that both are capable of forming long-term mammospheres with a grape-like morphology. Adherent and nonadherent cultures exhibited fast growth in vivo. Stem cell markers expressions showed that IPC-366 and SUM149 in adherent and nonadherent conditions has mesenchymal-like characteristics, E-cadherin and N-cadherin, was higher in adherent than in nonadherent cultures. Therefore, this study determines that both cell lines are similar and IPC-366 is a good model for the human and canine disease.
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    In vitro and in vivo effect of flutamide on steroid hormone secretion in canine and human inflammatory breast cancer cell lines
    (Veterinary and Comparative Oncology, 2017) Cáceres Ramos, Sara Cristina; Monsalve, Beatriz; Peña Fernández, Laura Luisa; Andrés Gamazo, Paloma Jimena De; Alonso‐Diez, Ángela; Illera Del Portal, Josefina María; Woodward, Wendy; Reuben, James; Silván Granado, Gema; Illera Del Portal, Juan Carlos
    The aim was to study the effects of flutamide on cell proliferation, in vivo tumour growth andsteroid production in canine and human IBC cell lines. IPC-366 and SUM149 cell cultures wereexposed to flutamide concentrations for 72 hours. Additionally, IPC-366 and SUM149 xeno-transplanted mice were treated subcutaneously with flutamide 3 times a week for 2 weeks.Steroid hormones determination in culture media, serum and tumour homogenates (pregneno-lone, progesterone, androstenedione, testosterone, dihydrotestosterone, 17β-oestradiol andoestrone sulphate) were assayed by EIA. in vitro cell proliferation percentages showed adecrease in all flutamide dosages in IPC-366 and SUM149. in vivo flutamide reduced tumoursize by 55% to 65%, and metastasis rates decreased. In treated groups, androgen levels in cul-ture media, serum and tumour homogenates were increased as oestrogen levels decreased. These results suggest that flutamide treatment inhibits cell proliferation and promotes tumourreduction by increasing androgen levels and also support future therapy approaches
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    Tumor Growth Progression in Ectopic and Orthotopic Xenografts from Inflammatory Breast Cancer Cell Lines
    (Veterinary Sciences, 2021) Cáceres Ramos, Sara Cristina; Alonso-Diez, Angela; Crespo, Belén; Peña Fernández, Laura Luisa; Illera Del Portal, Josefina María; Silván Granado, Gema; Andrés Gamazo, Paloma Jimena De; Illera Del Portal, Juan Carlos
    Xenografts can grow in immunosuppressed hosts, such as SCID mice, and tumor material can be injected into hosts either ectopically or orthotopically. Choosing the correct model to use is a crucial step in animal research. The aim of this study was to report the differences between ectopic and orthotopic xenografts in tumor progression, metastasis capacity, histological features, and steroid hormone profiles in xenografts from the cIMC (canine inflammatory mammary cancer) cell line IPC-366 and hIBC (human inflammatory breast cancer) cell line SUM149. To achieve this purpose, 40 female mice 6–8 weeks old were inoculated with IPC-366 and SUM149 cells subcutaneously (ectopic models) or into mammary fat pad (orthotopic models). Mice were monitored for tumor progression and appearance of metastases, and generated tumors were analyzed in terms of histological examination and steroid hormone production. The results revealed differences in tumor appearance and percentage of metastasis between ectopic and orthotopic models, which were higher in the ectopic xenografts from both cell lines. However, both models had similar characteristics of tumor progression, histological features, and steroid hormone secretion profiles. We show that the ectopic model can be validated as a good and useful model of tumor development in addition to, not contrary to, the orthotopic model in breast cancer research.
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    Survival time of dogs with inflammatory mammary cancer treated with palliative therapy alone or palliative therapy plus chemotherapy
    (Veterinary Record, 2009) Clemente, Mónica; Andrés Gamazo, Paloma Jimena De; Peña Fernández, Laura Luisa; Pérez Alenza, María De Los Dolores
    Seven of 30 female dogs diagnosed with inflammatory mammary cancer were given chemotherapy and palliative treatment, and the other 23 received only palliative treatment. The median survival time of the seven dogs given chemotherapy was 57 days, compared with 35 days for the 23 given only palliative treatment.
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    Project number: 263
    Diseño e implementación de un taller de aprendizaje transversal basado en la resolución de problemas en Histología y Anatomía Patológica Veterinarias
    (2020) Jiménez Martínez, María De Los Ángeles; Andrés Gamazo, Paloma Jimena De; Sánchez Maldonado, María Belén; García Palencia, María Del Pilar; Sánchez Pérez, María De Los Ángeles; García Fernández, Rosa Ana; Rollán Landeras, Eduardo Ricardo; González Huecas, Marta; Tabanera De Lucio, Enrique; Peña Fernández, Laura Luisa
    Mediante el desarrollo de un taller práctico empleando las técnicas de aprendizaje basado en la resolución de problemas y deducción de pruebas, los alumnos de tres asignaturas diferentes pero relacionadas (Histología Veterinaria, Anatomía Patológica General y Especial), del grado en veterinaria, participaron en su propio proceso de aprendizaje, trabajando en equipo, desarrollando habilidades deductivas y críticas al resolver y analizar supuestos reales, que les ayudarán a estar mejor preparados para su futuro profesional.