Person:
Parrado Quintela, Concepción

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First Name
Concepción
Last Name
Parrado Quintela
URI
https://hdl.handle.net/20.500.14352/81547
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
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2018 - 201922020 - 20222
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Now showing 1 - 4 of 4
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    Project number: 73
    Implementación de aplicaciones móviles como herramientas de enseñanza aprendizaje en Química Analítica
    (2018) Villalonga Santana, Reynaldo; Parrado Quintela, Concepción; Sánchez Sánchez, Alfredo; Reviejo García, Ángel Julio; Pérez Quintanilla, Damián; Díez Sánchez, Paula; Jiménez Falcao, Sandra
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    Dendrimers as Soft Nanomaterials for Electrochemical Immunosensors
    (Nanomaterials, 2019) Sánchez Sánchez, Alfredo; Villalonga, Anabel; Martínez García, Gonzalo; Parrado Quintela, Concepción; Villalonga Santana, Reynaldo
    Electrochemical immunosensors are antibody-based affinity biosensors with a high impact on clinical, environmental, food, and pharmaceutical analysis. In general, the analytical performance of these devices is critically determined by the materials and reagents used for their construction, signal production and amplification. Dendrimers are monodisperse and highly branched polymers with three-dimensional structures widely employed as “soft” nanomaterials in electrochemical immunosensor technology. This review provides an overview on the state-of-the-art in dendrimer-based electrochemical immunosensors, focusing on those using polyamidoamine and poly (propylene imine) dendrimers. Special emphasis is given to the most original methods recently reported for the construction of immunosensor architectures incorporating dendrimers, as well as to novel sensing approaches based on dendrimer-assisted signal enhancement strategies.
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    Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor
    (Biosensors, 2022) Villalonga Chico, Anabel; Parrado Quintela, Concepción; Diaz, Raúl; Sánchez Sánchez, Alfredo; Mayol Hornero, Beatriz; Martínez-Ruíz, Paloma; Vilela García, Diana; Villalonga Santana, Reynaldo
    Here we report a novel labeling strategy for electrochemical aptasensors based on enzymatic marking via supramolecular host–guest interactions. This approach relies on the use of an adamantane-modified target-responsive hairpin DNA aptamer as an affinity bioreceptor, and a neoglycoconjugate of β-cyclodextin (CD) covalently attached to a redox enzyme as a labeling element. As a proof of concept, an amperometric aptasensor for a carcinoembryonic antigen was assembled on screen-printed carbon electrodes modified with electrodeposited fern-like gold nanoparticles/graphene oxide and, by using a horseradish peroxidase-CD neoglycoenzyme as a biocatalytic redox label. This aptasensor was able to detect the biomarker in the concentration range from 10 pg/mL to 1 ng/mL with a high selectivity and a low detection limit of 3.1 pg/mL in human serum samples.
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    Amperometric aptasensor with sandwich-type architecture for troponin I based on carboxyethylsilanetriol-modified graphene oxide coated electrodes
    (Biosensors and Bioelectronics, 2021) Villalonga, Anabel; Estabiel, Itziar; Pérez-Calabuig, Ana ; Mayol Hornero, Beatriz; Parrado Quintela, Concepción; Villalonga Santana, Reynaldo
    A novel amperometric aptasensor for the specific detection of cardiac troponin I (cTnI) was constructed by using screen-printed carbon electrodes coated with a carboxyethylsilanetriol-modified graphene oxide derivative as transduction element. This novel carboxylic acid-enriched nanomaterial allows easy and high load immobilization of the capture aptamer molecules on the electrode surface. The biosensing interface was assembled by covalent attachment of an amino-functionalized DNA aptamer on the carboxylic acid-enriched electrode surface. The sensing approach relies on the specific recognition of cTnI by the aptamer and further assembly of a sandwich-type architecture with a novel aptamer-peroxidase conjugate as signaling element. The aptasensor was employed to detect the cardiac biomarker in the broad range from 1.0 pg/mL to 1.0 μg/mL with a detection limit of 0.6 pg/mL. This electroanalytical device also showed high specificity, reproducibility and stability, and was useful to quantify cTnI in reconstituted human serum samples.