Person:
Ochando González, María Dolores

First Name

María Dolores

Last Name

Ochando González

URI

https://hdl.handle.net/20.500.14352/83658

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Ciencias Biológicas

Area

Genética

Identifiers

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Now showing 1 - 10 of 12

Genetic polymorphism at the cytochrome oxidase I gene in Mediterranean populations of Batrocera oleae (Diptera: Tephritidae)
(Journal of Applied Entomology, 2013) Matallanas Peñas, Beatriz; Lantero, Esther; M'Saad, Meriem; Callejas Hervás, Carmen; Ochando González, María Dolores
The tephritid Bactrocera oleae (Gmelin) is a harmful pest of olive crops that cause important agricultural and economic losses in the Mediterranean area where 90% of the world olive trees are cultivated. The knowledge of the genetic diversity in insect pest species populations is critical for decisions concerning appropriate control management strategies. In the presnt work, the genetic variability within and among 7 populations -five from Spain, one from Italy and one from Tunisia-, was assessed by sequencing 1151 bp of the COI gene. A total of 21 haplotypes were observed. The intraspecific diversity was high, particularly in the Spanish populations (haplotype and nucleotide diversity 0.84 and 0.00137, respec tively). However, the genetic differentiation among the populations was low in the case of Spanish ones (Fst between 0 and 0.041), and higher – and statistically significant– when comparing with the Italian and Tuni sian samples. The haplotypes distribution and the PCoA analysis show three clear groups of populations: Spanish, Italian and Tunisian. The results might indicate the length of time elapsed since B. oleae became established in the Mediterranean region, the large effective sizes expected of its populations and the high gene flow among Iberian populations. The information could be relevant for integrated control programmes coordination.
Genetic structure of Spanish populations of Ceratitis capitata revealed by RAPD and ISSR markers: implications for resistance management
(Spanish Journal of Agricultural Research, 2012) Beroiz Remírez, Beatriz; Ortego, Félix; Callejas Hervás, Carmen; Hernández-Crespo, Pedro; Castañera, Pedro; Ochando González, María Dolores
The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is considered one of the most economically damaging pests of citrus orchards in Spain. The characterization of C. capitata population structure, at a large geographical scale, by using a combination of RAPD and ISSR markers can allow analyzing the genetic variability of this species, and provide some insight in decision making for resistance management, recently recorded in Spain. We compared six Spanish populations along the Mediterranean area (Gerona, Amposta, Tortosa, Castellón, Valencia and Málaga) with populations from other geographical areas where this pest is widely distributed (Africa, Middle East, South America and Atlantic Islands) and two laboratory strains. The results obtained with both types of molecular markers were similar. A dendrogram based on Nei genetic distances showed that all Mediterranean Spanish populations, except the population collected in Gerona, were clearly separated from the rest. However, no clear differentiation among Spanish populations was found, probably as a result of the high levels of gene flow (Nm value of 2.8 for RAPD and 3.9 for ISSR). Implications of these findings on resistance management of C. capitata are discussed.
A genetic approach to spanish populations of the threatened Austropotamobius italicus located at three different scenarios
(The Scientific World Journal, 2012) Matallanas Peñas, Beatriz; Callejas Hervás, Carmen; Ochando González, María Dolores
Spanish freshwater ecosystems are suffering great modification and some macroinvertebrates like Austropotamobius italicus, the white-clawed crayfish, are threatened. This species was once widely distributed in Spain, but its populations have shown a very strong decline over the last thirty years, due to different factors. Three Spanish populations of this crayfish—from different scenarios—were analysed with nuclear (microsatellites) and mitochondrial markers (COI and 16S rDNA). Data analyses reveal the existence of four haplotypes at mitochondrial level and polymorphism for four microsatellite loci. Despite this genetic variability, bottlenecks were detected in the two natural Spanish populations tested. In addition, the distribution of the mitochondrial haplotypes and SSR alleles show a similar geographic pattern and the genetic differentiation between these samples is mainly due to genetic drift. Given the current risk status of the species across its range, this diversity offers some hope for the species from a management point of view.
Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera: Tephritidae)
(Spanish Journal of Agricultural Research, 2017) Lantero, Esther; Matallanas Peñas, Beatriz; Ochando González, María Dolores; Pascual, Susana; Callejas Hervás, Carmen
Bactrocera oleae, the olive fruit fly, is a major pest of olive (Olea europaea L.) trees worldwide. Its presence can cause important losses, with consequences for the economies of countries that produce and export table olives and olive oil. Efforts to control olive fruit fly populations have, however, been insufficient. Now more than ever, environmentally friendly alternatives need to be considered in potential control programs. Generalist predators could provide a way of managing this pest naturally. However, the identification of candidate predator species is essential if such a management system is to be introduced. The present paper describes a set of species-specific primers for detecting the presence of B. oleae DNA in the gut of predatory arthropods. All primers were tested for checking cross-reactive amplification of other fruit fly DNA and evaluated in heterospecific mixes of nucleic acids. All were found to be very sensitive for B. oleae. Subsequent feeding trials were conducted using one of the most abundant species of ground dwelling carabids in olive groves in south-eastern Madrid, Spain. These trials allowed determining that 253F-334R and 334F-253R primer pairs had the highest detection efficiency with an ID50 of around 78 h. These primers therefore provide a very useful tool for screening the gut contents of potential predators of B. oleae, and can thus reveal candidate species for the pest's biological control.
Fine-tuning of a COI PCR-RFLP assay for fast genetic characterization of Spanish white-clawed crayfish
(Knowledge and Management of Aquatic Ecosystems, 2016) Matallanas Peñas, Beatriz; Ochando González, María Dolores; Callejas Hervás, Carmen
The white-clawed crayfish is endemic to western and southern Europe and its population has dramatically decreased in the last few decades. The latest reports on this species have shown that the genetic variability of Spanish populations displays a clear geographic pattern, with two main genetic groups across its range of distribution. To ensure the preservation of this endangered species, specimens for restocking purposes should be chosen, ideally, accordingly to their genetic characteristics. Here, we propose a PCR-RFLP assay developed for this purpose. Digestion of a fragment of the mitochondrial cytochrome oxidase subunit I gene – with two restriction enzymes, HpaI and CviAII - differentiates the most common haplotypes identified in Spain. This technique is a useful, low-cost and rapid method to facilitate the genetic characterization of Spanish white-clawed crayfish and, in doing so, to incorporate genetic information into conservation plans for this species.
Mitochondrial DNA variability in Spanish populations of A. italicus inferred from the analysis of a COI region
(Knowledge and Management of Aquatic Ecosystems, 2011) Matallanas, Beatriz; Ochando González, María Dolores; Vivero, Almudena; Beroiz Remírez, Beatriz; Alonso, Fernando; Callejas, Carmen; Callejas Hervás, Carmen
Austropotamobius italicus was once widely distributed throughout most of the country’s limestone basins in Spain. But its populations have shown a very strong decline over the last thirty years, due to different factors. Thus, the species now enjoys protection under regional, national and international legislation. Therefore, knowledge of the levels and patterns of distribution of genetic diversity in crayfish populations is critical when making conservation management decisions. In the present work, the current genetic structure of Spanish populations of white-clawed crayfish, A. italicus, was analyzed. Eleven Spanish populations and an Italian sample were studied through an 1184 bp-lentgh sequence of cytochrome oxidase subunit I mitochondrial gene. Data analysis revealed the existence of eight haplotypes in the Iberian Peninsula, the highest diversity reported to date in Spanish crayfish. Also a substantial genetic differentiation among populations was found, with a clear geographic pattern. The genetic variability found in these populations is similar to, and even higher, than that reported in previous studies on other Spanish and European populations of A. italicus. Thus, given the current risk status of the species across its range, this variability in certain populations offers some hope for the species from a management point of view.
The white-clawed crayfish in Spain—reply to Clavero and Centeno-Cuadros
(Organisms, diversity and evolution, 2016) Matallanas Peñas, Beatriz; Ochando González, María Dolores; Alonso, F.; Callejas Hervás, Carmen
This short communication is only intended to point out briefly and objectively some of the comments, appreciations, and sometimes misinterpretations made by Clavero and CentenoCuadros in their criticism to our article on the phylogeography of the white-clawed crayfish in Spain, recently published in this journal. For those skilled in the discipline of phylogeography, it is well known to be a multidisciplinary science. Therefore, we disagree with the argument of monodisciplinary approach given by these authors. The work of Matallanas et al. 2016, as all our previous ones, has no intention, no bias, or directionality. It clearly aims what the title synthesizes, an update of genetic information for the white-clawed crayfish in Spain. To achieve this, we have been the first to use two mitochondrial markers, the longest so far used, in a wide and representative sample of Iberian crayfish. A sample of crayfish fromNorthern Italy was just used as outgroup. The design is, therefore, very suitable and appropriate for the question posed.
New information about genetic structure of B. oleae species revealed by ISSR markers
(Proceedings of the IOBC/WPRS Working Group "Integrated Protection of Olive Crops", 2012) Callejas Hervás, Carmen; Ochando González, María Dolores; Hernández, Sofía; Matallanas, Beatriz; D. Perdikis, P. Weintraub, A. Lucchi
The olive fruit fly, Bactrocera oleae, is a major pest of olive crops and its expansion isrestricted to the geographic areas where olive trees are grown. The knowledge of the within andbetween populations genetic variability can help to understand the history of a species andmonitoring the origin and spread of invading populations. Such information could be crucial todefine appropriate strategies for eradication or control.In the present work, PCR amplification ofinter-simple sequence repeats (ISSR technique) was applied to the analysis of the geneticvariability of four Iberian populations of B. oleae. Flies from four different geographical areas,representing the Iberian distribution range of the species, were collected by harvesting infestedfruit and allowing the larvae to pupate in the laboratory. Four random primers (817, 820, 820,847, UBC primer set no. 9) were used to assess their genetic variation. The results showconsiderable levels of genetic polymorphism in the analysed samples, ranging from 78.5% to82.1%. Regarding the distribution of this variability, most of the genetic variation was foundwithin populations (92.6%). Likewise, a substantial level of gene flow (5.36) was deduced. Thus,ISSR are highly sensitive markers for variability detection in B. oleae and could help to answerfundamental questions related to the population structure and dynamics of the fly and, hence, toimprove management control.
Update of genetic information for the white-clawed crayfish in Spain, with new insights into its population genetics and origin
(Organisms Diversity and Evolution, 2016) Matallanas Peñas, Beatriz; Ochando González, María Dolores; Alonso, F.; Callejas Hervás, Carmen
The white-clawed crayfish is endemic to western and southern Europe and its populations have decreased over recent decades. Spanish populations are generally poorly represented in scientific reports and are usually studied only with a single molecular marker. Here, we use two mitochondrial markers (cytochrome oxidase subunit I and rDNA 16S genes) to examine levels and patterns of genetic structure across the range of the species’ distribution in Spain. Data reveal the existence of two main genetic groups of white-clawed crayfish in Spain with the Ebro basin as a possible contact zone. Processes occurred in historical and recent times, such as genetic drift and translocations, contribute greatly to this genetic structure. Levels of genetic variability and genetic structure of Spanish populations together with demographic inferences suggest that the species established in the Iberian Peninsula, at least since the Late Pleistocene. Knowing the true origin of the Spanish populations is crucial when deciding upon the management policies that should be followed. Given the lack of any clear evidence against its indigenous status, we propose that current protection and conservation measures should be maintained. From a management point of view, we suggest that Spanish population should be considered as a single evolutionary significant unit (ESU) with two management units (MUs) corresponding with the genetic clusters detected in the present study.
Preserving the biodiversity of freshwater ecosystems in a scenario of increasing desertification: lessons from genetics
(Handbook of Environmental Research, 2010) Beroiz Remírez, Beatriz; Callejas Hervás, Carmen; Ochando González, María Dolores; Alonso, Fernando; Vivero, Almudena; Matallanas, Beatriz; Edelstein, Aurel & Bär, Dagmar
The European Union is faced with the challenge of environmental alteration. In recent years, human activities and accelerating climate change have had a great impact in many regions, with the freshwater ecosystems of Mediterranean countries being hit particularly hard. Spain has the greatest biodiversity of Europe. The country’s maritime barriers, the Pyrenees, and its orographic and climatic peculiarities, invest it with its unique biogeographic characteristics, which together have led to the appearance of many endemic freshwater species. However, the freshwater ecosystems of Spain are suffering great modification at the hands of climate change (some Mediterranean regions now receive 20% less rain than a century ago), environmental degradation, habitat fragmentation, the rise in human demand for water, and a range of human activities; together these factors have contributed to a notable increase in the size of Spain’s arid and semiarid regions, and to changes in its biodiversity. The country’s aquatic biodiversity is now threatened, and the Spanish Ministry of the Environment and the Rural and Marine Environments and the Biodiversity Foundation have seen the need to launch several biodiversity conservation programmes. A major goal of such programmes should be to preserve genetic variability. Indeed, knowledge of the levels and patterns of distribution of genetic diversity in populations of freshwater species is critical when making conservation management decisions. Our group is conducting research into the genetic variation, and its distribution, in two cornerstone freshwater groups: the genus Barbus, which has the greatest species diversity of all Iberian fish genera, and the white-clawed crayfish Austropotamobius pallipes. The latter was once widely distributed throughout most of the country’s limestone basins; now much more rare, it enjoys protection under regional, national and European legislation. The ecological importance and the conservation interest surrounding the above organisms led us to analyse the genetic variability of their populations over their entire distribution ranges. To obtain as complete a picture as possible, nuclear (RAPD, ISSR) and mitochondrial (coI, cyt b and 16S genes) molecular markers were studied. Evidence is provided suggesting that the genetic variability in barbel species is low. The genetic variation seen for A. Pallipes, however, was slightly higher than expected, allowing some hope of its recovery. We are also generating a genetic database for both groups; this could serve as an aid to managers and policy-makers involved in freshwater conservation.