Person: Ochando González, María Dolores
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First Name
María Dolores
Last Name
Ochando González
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Department
Area
Genética
Identifiers
3 results
Search Results
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Item Fine-tuning of a COI PCR-RFLP assay for fast genetic characterization of Spanish white-clawed crayfish(Knowledge and Management of Aquatic Ecosystems, 2016) Matallanas Peñas, Beatriz; Ochando González, María Dolores; Callejas Hervás, CarmenThe white-clawed crayfish is endemic to western and southern Europe and its population has dramatically decreased in the last few decades. The latest reports on this species have shown that the genetic variability of Spanish populations displays a clear geographic pattern, with two main genetic groups across its range of distribution. To ensure the preservation of this endangered species, specimens for restocking purposes should be chosen, ideally, accordingly to their genetic characteristics. Here, we propose a PCR-RFLP assay developed for this purpose. Digestion of a fragment of the mitochondrial cytochrome oxidase subunit I gene – with two restriction enzymes, HpaI and CviAII - differentiates the most common haplotypes identified in Spain. This technique is a useful, low-cost and rapid method to facilitate the genetic characterization of Spanish white-clawed crayfish and, in doing so, to incorporate genetic information into conservation plans for this species.Item Genetic structure of Spanish white-clawed crayfish (Austropotamobius pallipes) populations as determined by RAPD analysis: reasons for optimism(Aquatic conservation: marine and freshwater ecosystems, 2008) Beroiz Remírez, Beatriz; Callejas Hervás, Carmen; Alonso, Fernando; Ochando González, María Dolores1. Spanish populations of the white-clawed crayfish have declined sharply over the last three decades. Although Austropotamobius pallipes was once widely distributed and very abundant in most of the limestone basins of the country, outbreaks of crayfish plague since 1978 have reduced its populations, and now only some 500–600 small populations are left. 2. Consequently, the species now enjoys protection under national legislation. Management decisions regarding the conservation of a threatened species require an understanding of the genetic structure of its populations. 3. Using random amplified polymorphic DNA (RAPD) fingerprinting the genetic variability of 11 populations of A. pallipes was assessed over the species' range in Spain, and their phylogenetic relationships determined. 4. Substantial genetic differentiation was detected among the populations tested; no clear relationship was found between patterns of genetic variability and hydrological basin. The RAPD markers showed the degree of genetic variability of these populations to be similar to, and in some cases slightly higher than, that reported in previous studies on other Spanish and European populations of A. pallipes. 5. The results offer hope for the recovery of this species in Spain, and provide information that might be useful in the management of crayfish reintroduction programmes.Item Update of genetic information for the white-clawed crayfish in Spain, with new insights into its population genetics and origin(Organisms Diversity and Evolution, 2016) Matallanas Peñas, Beatriz; Ochando González, María Dolores; Alonso, F.; Callejas Hervás, CarmenThe white-clawed crayfish is endemic to western and southern Europe and its populations have decreased over recent decades. Spanish populations are generally poorly represented in scientific reports and are usually studied only with a single molecular marker. Here, we use two mitochondrial markers (cytochrome oxidase subunit I and rDNA 16S genes) to examine levels and patterns of genetic structure across the range of the species’ distribution in Spain. Data reveal the existence of two main genetic groups of white-clawed crayfish in Spain with the Ebro basin as a possible contact zone. Processes occurred in historical and recent times, such as genetic drift and translocations, contribute greatly to this genetic structure. Levels of genetic variability and genetic structure of Spanish populations together with demographic inferences suggest that the species established in the Iberian Peninsula, at least since the Late Pleistocene. Knowing the true origin of the Spanish populations is crucial when deciding upon the management policies that should be followed. Given the lack of any clear evidence against its indigenous status, we propose that current protection and conservation measures should be maintained. From a management point of view, we suggest that Spanish population should be considered as a single evolutionary significant unit (ESU) with two management units (MUs) corresponding with the genetic clusters detected in the present study.