Person:
Medina Bujalance, Rafael

First Name

Rafael

Last Name

Medina Bujalance

URI

https://hdl.handle.net/20.500.14352/74313

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Ciencias Biológicas

Department

Biodiversidad, Ecología y Evolución

Area

Botánica

Identifiers

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Now showing 1 - 10 of 22

Unnoticed diversity within the disjunct moss Orthotrichum tenellum s.l. validated by morphological and molecular approaches
(Taxon, 2013) Medina Bujalance, Rafael; Francisco Lara; Bernard Goffinet; Ricardo Garilleti; Vicente Mazimpaka
Transoceanic disjunctions are thought to be common in species of bryophytes. While much effort focuses on identifying the causes of these disjunctions, few studies test the assumption that the disjunct populations are conspecific. The moss Orthotrichum tenellum is considered to be disjunct between western North America and the western Old World. A thorough morphological revision together with molecular analyses using four plastid (atpB-rbcL, rps4, trnG, trnL-F) and two nuclear loci (adk, ort-LFY) reveals that this putative taxon is composed of six well-supported species, each of them diagnosable by morphological and phylogenetic criteria. Furthermore, these species, which were previously treated as a single, morphologically variable taxon, belong to different lineages within the genus and are the result of separated evolutionary histories. Only two of them (O. tenellum s.str., O. norrisii) are sister species that may have resulted from speciation following a transatlantic disjunction. Today, all species are either restricted to the Old World (i.e., O. tenellum s.str., O. comosum sp. nov.) or endemic to North America (i.e., O. coulteri, O. norrisii, O cucullatum sp. nov., O. franciscanum sp. nov.). This work emphasizes the relevance of integrative approaches to solve taxonomic problems and to provide more solid bases for biodiversity assessment.
Nuevas citas briológicas de España y Portugal, 2023
(Boletín de la Sociedad Española de Briología, 2023) Patiño, Jairo; Calleja, Juan A.; Cuerdo, Macarena; Olmo, David G. del; Horcajada, David; Mairal, Mario; Medina Bujalance, Rafael; Muñoz, Álvaro; Santos, Guillermo
Northeastern Turkey, an unnoticed but very important area for the Orthotrichaceae (Bryophyta)
(Bryophyte Systematics, Phytodiversity, Phytosociology and Ecology. Festschrift in honour of Professor Dr. Harald Kürschner, 2010) Lara, Francisco; Mazimpaka, Vicente; Medina Bujalance, Rafael; Caparrós, Rut; Garilleti, Ricardo; Schweizerbart science publishers
In the course of a prospecting trip in square A4 in Turkey, the authors have recorded a total of 29 species of Orthotrichaceae. This increases by 19 the number of species in this moss family in the area. Among the discoveries are 11 new records for Turkey: Orthotrichum callistomum, O. consobrinum, O. crenulatum, O. hispanicum, O. rogeri, O. sordidum, O. stellatum, O. vladikavkanum, Ulota coarctata, U. rehmannii and Zygodon dentatus, some of them previously unknown in Asia. Furthermore, Orthotrichum microcarpum is reported for the fi rst time from Anatolia and new populations of some other little known mosses in Turkey are documented: O. alpestre, O. patens, O. scanicum, and O. vittii. Data on distribution and habitat in the prospected territory are provided for each of the species. The novelties are discussed and comments provided on global distribution and key characters for differentiation, in some cases accompanied by illustrations. In the light of the new data, North-eastern Turkey is shown to be one of the most diverse and interesting areas for Orthotrichaceae in the Palaearctic.
Epiphytic bryophytes in harsh environments: theJuniperus thuriferaforests
(Journal of Bryology, 2010) Medina Bujalance, Rafael; Francisco Lara; Belén Albertos; Isabel Draper; Ricardo Garilleti; Vicente Mazimpaka
The forests of Juniperus thurifera are peculiar ecosystems that typically grow on mountains and highplateaux of the western Mediterranean basin with dry and continental climates. Some previous surveys suggested that these forests house a rather distinctive epiphytic bryophyte flora. Epiphytic bryophyte communities were systematically sampled in 19 representative juniper forests, for the first time spanning all the distribution area of this conifer. The flora consists of 44 species (32 acrocarpous mosses, 10 pleurocarpous mosses and 2 liverworts). Orthotrichum species are the most frequent and abundant in most of the sampled localities, including some uncommon taxa such as Orthotrichum vittii which shows a clear association with J. thurifera. Epiphytic bryophyte communities on this species were highly homogeneous, although they can be arranged into different groups in response to environmental conditions. Typically, the epiphytic communities of the Mediterranean juniper forests comprise a distinct combination of xerophytic taxa that enhances the interest of these ecosystems and provides new fields for their research and conservation.
Orthotrichum Norrisii (Orthotrichaceae), A New Epiphytic Californian Moss
(Bryologist, 2008) Lara, Francisco; Mazimpaka, Vicente; Garilleti, Ricardo; Medina Bujalance, Rafael
A new Orthotrichum species, O. norrisii, is described from California. The new taxon is recognized by a unique combination of sporophytic characters: capsule narrowly cylindrical and not contracted below mouth when dry, exothecial bands narrow and formed by 2(–3) cell rows, peristome with eight pairs of exostome teeth and eight endostome segments, stomata cryptopore and located in the lower part of the urn, lid plane and rostrate and vaginula hairy. Widely distributed throughout California, the new taxon has been overlooked and confused with O. tenellum.
Orthotrichum pilosissimum (Orthotrichaceae), a new moss from arid areas of Nevada with unique axillary hairs
(Bryologist, 2011) Medina Bujalance, Rafael; Francisco Lara; Vicente Mazimpaka; James R. Shevock; Ricardo Garilleti
Orthotrichum pilosissimum is described from herbarium material and recently collected specimens in the state of Nevada. Its specific epithet refers to the diagnostic hyper-developed axillary hairs, which are frequently longer than 1 mm, multiseriate and branched, covering most of the stems surface and sometimes protruding from the foliose shoots. These hairs are not only unique in the genus but also among mosses, providing a reliable diagnostic character for the new taxon. Additional gametophytic and sporophytic characters that contribute to its taxonomic delimitation are also given.
On the presence of Orthotrichum patens Bruch & Brid. in the Iberian Peninsula
(Cryptogamie, Bryologie, 2008) Lara, Francisco; Medina Bujalance, Rafael; Garilleti, Ricardo; Mazimpaka, Vicente
Orthotrichum patens Bruch & Brid., considered to be a rare moss in the Iberian Peninsula, is reported from two localities in Northern Spain (Asturias and Navarra). After revision of the herbarium material corresponding to previous records of this species in the Iberian Peninsula, we conclude that most of them (Asturias, Álava, León and La Rioja provinces) are erroneous. However, two early records (Sierra Nevada and Sierra de Ancares) remain uncertain since the corresponding herbarium material has not been found. We also provide details of two new localities of Orthotrichum patens in Romania and two in Turkey, where the species has been poorly recorded. Finally, we discuss the characters that allow the discrimination of Orthotrichum patens from related species, especially from O. stramineum Hornsch., the moss that has most often been confused with it in Spain.
Origin and fate of the single‐island endemic moss Orthotrichum handiense
(Journal of Biogeography, 2013) Jairo Patiño; Medina Bujalance, Rafael; Alain Vanderpoorten; Juana M. González‐Mancebo; Olaf Werner; Nicolas Devos; Rubén G. Mateo; Francisco Lara; Rosa M. Ros; Peter Linder
Aim. Our aims were: to determine the evolutionary origin of the single-island endemic moss Orthotrichum handiense; to assess whether its endemicity results from a recent origin or founder event, a loss of dispersal ability, specific habitat requirements, or contraction of a formerly wider range; and to make predictions about its ability to face ongoing climate change. Location. Fuerteventura, Canary Islands. Methods. The evolutionary origin of O. handiense was determined by phylogenetic analysis and molecular dating. The spatial genetic structure and demographic history of 48 individuals of O. handiense were analysed with inter-simple sequence repeat (ISSR) markers through analyses of molecular variance, Mantel tests, mismatch distributions and bottleneck analyses. The macroclimatic envelope of the species was modelled using an ensemble approach of eight species distribution models, and its suitable areas predicted for extant, past (21 ka) and future (2050, 2080) conditions. Results. Orthotrichum handiense was resolved as sister to the Californian O. underwoodii and their most recent common ancestor was dated to early Miocene–Pliocene. ISSR analyses revealed extremely low levels of genetic diversity and provided evidence for a recent bottleneck and for isolation-by-distance at the local scale. The different models investigated all pointed to the mismatch between the limited extant distribution and the extent of macroclimatically suitable areas. All models predicted climatic unsuitability on Fuerteventura and Lanzarote at 21 ka, but were conflicting in other areas. A dramatic reduction and loss of suitable areas were predicted for 2050 and 2080, respectively. Main conclusions The phylogenetic position of O. handiense points to a long-distance dispersal event from a western North American ancestor and emphasizes the differences in the origin of Macaronesian endemic bryophytes and angiosperms. The predicted absence of suitable macroclimatic conditions in Fuerteventura at 21 ka supports the hypothesis of a palaeoendemic origin and a recent founder event in Fuerteventura, consistent with the low levels of genetic diversity and with evidence for a recent bottleneck. While the biogeographical history of the species hence involves major dispersal events over periods of tens of thousands of years, its ability to respond quickly to predicted climate change during the next few decades is questioned.
Phylogenomic delineation of Physcomitrium (Bryophyta: Funariaceae) based on targeted sequencing of nuclear exons and their flanking regions rejects the retention of Physcomitrella, Physcomitridium and Aphanorrhegma
(Journal of Systematics and Evolution, 2019) Medina Bujalance, Rafael; Johnson, Matthew G.; Liu, Yang ; Wickett, Norman J. ; Shaw, A. Jonathan ; Goffinet, Bernard
Selection on spore dispersal mechanisms in mosses is thought to shape the transformation of the sporophyte. The majority of extant mosses develop a sporangium that dehisces through the loss of an operculum, and regulates spore release through the movement of articulate teeth, the peristome, lining the capsule mouth. Such complexity was acquired by the Mesozoic Era, but was lost in some groups during subsequent diversification events, challenging the resolution of the affinities for taxa with reduced architectures. The Funariaceae are a cosmopolitan and diverse lineage of mostly annual mosses, and exhibit variable sporophyte complexities, spanning from long, exerted, operculate capsules with two rings of well-developed teeth, to capsules immersed among maternal leaves, lacking a differentiated line of dehiscence (i.e., inoperculate) and without peristomes. The family underwent a rapid diversification, and the relationships of taxa with reduced sporophytes remain ambiguous. Here, we infer the relationships of five taxa with highly reduced sporophytes based on 648 nuclear loci (exons complemented by their flanking regions), based on inferences from concatenated data and concordance analysis of single gene trees. Physcomitrellopsis is resolved as nested within one clade of Entosthodon. Physcomitrella s. l., is resolved as a polyphyletic assemblage and, along with its putative relative Aphanorrhegma, nested within Physcomitrium. We propose a new monophyletic delineation of Physcomitrium, which accommodates species of Physcomitrella and Aphanorrhegma. The monophyly of Physcomitrium s. l. is supported by a small plurality of exons, but a majority of trees inferred from exons and their adjacent non-coding regions.
HybPiper: Extracting coding sequence and introns for phylogenetics from high‐throughput sequencing reads using target enrichment
(Application in Plant Sciences, 2016) G. Johnson, Matthew ; Gardner, Elliot M. ; Liu, Yang; Medina Bujalance, Rafael; Goffinet, Bernard; Shaw, A. Jonathan; Zerega, Nyree J. C.; Wickett, Norman J.
Premise of the study: Using sequence data generated via target enrichment for phylogenetics requires reassembly of highthroughput sequence reads into loci, presenting a number of bioinformatics challenges. We developed HybPiper as a userfriendly platform for assembly of gene regions, extraction of exon and intron sequences, and identification of paralogous gene copies. We test HybPiper using baits designed to target 333 phylogenetic markers and 125 genes of functional significance in Artocarpus (Moraceae). Methods and Results: HybPiper implements parallel execution of sequence assembly in three phases: read mapping, contig assembly, and target sequence extraction. The pipeline was able to recover nearly complete gene sequences for all genes in 22 species of Artocarpus. HybPiper also recovered more than 500 bp of nontargeted intron sequence in over half of the phylogenetic markers and identified paralogous gene copies in Artocarpus. Conclusions: HybPiper was designed for Linux and Mac OS X and is freely available at https://github.com/mossmatters/HybPiper.