Person:
Ortiz Vera, Luis Tomás

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First Name
Luis Tomás
Last Name
Ortiz Vera
URI
https://hdl.handle.net/20.500.14352/80615
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Producción Animal
Area
Producción Animal
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UCM identifierORCIDScopus Author IDWeb of Science ResearcherIDDialnet IDGoogle Scholar ID

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2014 - 20172
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End date: 2019 × Start date: 2010 ×

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    Validation of an Inductively Coupled Plasma-Optical Emission Spectrometry Method for the Determination of Major Elements in Farmed Rainbow Trout (Oncorhynchus mykiss)
    (Journal of analytical chemistry, 2017) Velasco Villar, Susana; Ortiz Vera, Luis Tomás; Rodríguez, M. L.; Rebolé Garrido, Almudena; Treviño, J.; Benito, T.; López Andrés, Sol; Gómez-Pinilla, María Isabel
    An inductively coupled plasma−optical emission spectrometry method was optimized and validated for the determination of major elements (Ca, K, Mg, Na and P) in cultivated freshwater fish (rainbow trout Oncorhynchus mykiss). The method was validated by analysis of a Certified Reference Material, consisting in a frozen tissue homogenate from lake trout (Salvelinus namaycush namaycush). The linearity of this method was very good, as evidenced by the coefficients of correlation (r) for calibration graphs that were higher than 0.9999 in all cases and by linearity test (response factor <5% and relative calibration graph slope <2%). Accuracy, expressed as relative recovery (%) in comparison with certified concentration ranged from 100 to 109%, and precision, expressed as residual standard deviation (%) ranged from 1.2 to 6.5% (repeatability) and from 1.0 to 9.6% (reproducibility). The limit of quantification ranged from 4 ng/mL (Ca and Mg) to 203 ng/mL (P). The optimized method was applied to major element determination in skin and muscle samples from rainbow trout fillets.
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    Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens
    (Plos One, 2014) Sevane Fernández, Natalia; Bialade, Federica; Velasco Villar, Susana; Rebolé Garrigós, Almudena; Rodríguez Membibre, María Luisa; Ortiz Vera, Luis Tomás; Cañón Ferreras, Francisco Javier; Dunner Boxberger, Helene Susana; te Pas Marinus FW
    Inclusion of prebiotics in the diet is known to be advantageous, with positive influences both on health and growth. The current study investigated the differences in the hepatic transcriptome profiles between chickens supplemented with inulin (a storage carbohydrate found in many plants) and controls. Liver is a major metabolic organ and has been previously reported to be involved in the modification of the lipid metabolism in chickens fed with inulin. A nutrigenomic approach through the analysis of liver RNA hybridized to the Affymetrix GeneChip Chicken Genome Array identified 148 differentially expressed genes among both groups: 104 up-regulated (≥1.4-fold) and 44 down-regulated (≤0.6-fold). Quantitative real-time PCR analysis validated the microarray expression results for five out of seven genes tested. The functional annotation analyses revealed a number of genes, processes and pathways with putative involvement in chicken growth and performance, while reinforcing the immune status of animals, and fostering the production of long chain fatty acids in broilers supplemented with 5 g of inulin kg−1 diet. As far as we are aware, this is the first report of a microarray based gene expression study on the effect of dietary inulin supplementation, supporting further research on the use of this prebiotic on chicken diets as a useful alternative to antibiotics for improving performance and general immunity in poultry farming, along with a healthier meat lipid profile.