Person:
García García, María De La Concepción

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First Name
María De La Concepción
Last Name
García García
URI
https://hdl.handle.net/20.500.14352/84613
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Area
Microbiología
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2019 - 201912020 - 20222
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Author: Lago Femia, Eva de × Start date: 2016 ×

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Now showing 1 - 3 of 3
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    Project number: 270
    Puesta en marcha del curso on line “Envejecimiento y enfermedades neurodegenerativas” en la plataforma Miriadax
    (2019) Lago Femia, Eva de; Sagredo Ezquioga, Onintza; García García, María De La Concepción; Hernández Galvez, María Luz; Gómez Ruiz, María; Ramos Atance, José Antonio; Fernandez Ruiz, José Javier; Rodríguez Cueto, Carmen; de Castro Vitores, Jacinto; López Blanco, Olga; Esteban Cubero, Amparo; Satta, Valentina; Espejo Porras, Francisco; Santos-García, Irene; García Toscano, Laura; Alonso Gómez, Cristina
    Este informe detalla los objetivos, actividades y métodos llevados a cabo durante la ejecución de un proyecto de innovación docente que tiene como objetivo implementar un curso online en abierto sobre las enfermedades neurodegenerativas y el envejecimiento.
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    BiP Heterozigosity Aggravates Pathological Deterioration in Experimental Amyotrophic Lateral Sclerosis
    (International Journal of Molecular Sciences, 2021) Gómez Almería, Marta; Burgaz García-Oteyza, Sonia; Costas Insua, Carlos; Rodríguez Cueto, Carmen; Santos García, Irene; Rodríguez Crespo, Ignacio; García García, María De La Concepción; Guzmán Pastor, Manuel; Lago Femia, Eva de; Fernández Ruiz, Javier
    In the present study, we investigated the involvement of the chaperone protein BiP (also known as GRP78 or Hspa5), a master regulator of intracellular proteostasis, in two mouse models of neurodegenerative diseases: amyotrophic lateral sclerosis (ALS) and Parkinson’s disease (PD). To this end, we used mice bearing partial genetic deletion of the BiP gene (BiP+/− mice), which, for the ALS model, were crossed with mutant SOD1 (mSOD1) transgenic mice to generate mSOD1/BiP+/− double mutant mice. Our data revealed a more intense neurological decline in the double mutants, reflected in a greater deterioration of the neurological score and rotarod performance, with also a reduced animal survival, compared to mSOD1 transgenic mice. Such worsening was associated with higher microglial (labelled with Iba-1 immunostaining) and, to a lesser extent, astroglial (labelled with GFAP immunostaining) immunoreactivities found in the double mutants, but not with a higher loss of spinal motor neurons (labelled with Nissl staining) in the spinal cord. The morphological analysis of Iba-1 and GFAP-positive cells revealed a higher presence of activated cells, characterized by elevated cell body size and shorter processes, in double mutants compared to mSOD1 mice with normal BiP expression. In the case of the PD model, BiP+/− mice were unilaterally lesioned with the parkinsonian neurotoxin 6-hydroxydopamine (6-OHDA). In this case, however, we did not detect a greater susceptibility to damage in mutant mice, as the motor defects caused by 6-OHDA in the pole test and the cylinder rearing test, as well as the losses in tyrosine hydroxylase-containing neurons and the elevated glial reactivity (labelled with CD68 and GFAP immunostaining) detected in the substantia nigra were of similar magnitude in BiP+/− mice compared with wildtype animals. Therefore, our findings support the view that a dysregulation of the protein BiP may contribute to ALS pathogenesis. As BiP has been recently related to cannabinoid type-1 (CB1) receptor function, our work also opens the door to future studies on a possible link between BiP and the neuroprotective effects of cannabinoids that have been widely reported in this neuropathological context. In support of this possibility, preliminary data indicate that CB1 receptor levels are significantly reduced in mSOD1 mice having partial deletion of BiP gene.
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    Project number: 259
    Uso la plataforma H5P de creación de contenido interactivo como herramienta para la creación de un laboratorio de bioquímica virtual e interactivo
    (2022) Sagredo Ezquioga, Onintza; Lago Femia, Eva de; García García, María De La Concepción; Navarro Gonzalez de Mesa, Elisa; Gómez Cañas, María; Rodríguez Cueto, Carmen; Satta, Valentina; Hernández Fisac, Inés
    En este proyecto de innovación docente está diseñado para que el alumnado se acerque a un laboratorio de bioquímica virtual e interactivo y conozca los diferentes métodos y técnicas experimentales que se utilizan en ciertos análisis clínicos.