Person: González Burgos, Elena María
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First Name
Elena María
Last Name
González Burgos
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Farmacología, Farmacognosia y Botánica
Area
Farmacología
Identifiers
9 results
Search Results
Now showing 1 - 9 of 9
- Project number: 249
Item Flipped Learning y Web App de Plantas Medicinales Nuevas estrategias educativas aplicadas al ámbito de la enseñanza en la asignatura de Farmacognosia y Fitoterapia(2017) Gómez-Serranillos Cuadrado, María Pilar; González Burgos, Elena María; Fernández Moriano, Carlos; Iglesias Peinado, Irene; Gavilán García, Rosario Gloria; Garcia Broncano, Rosario Item Current knowledge on Parmelia genus: Ecological interest, phytochemistry, biological activities and therapeutic potential(2019) González Burgos, Elena María; Fernández Moriano, Carlos; Gómez-Serranillos Cuadrado, María PilarParmelia Acharius is one of the most representative genera within Parmeliaceae family which is the largest and the most widespread family of lichen-forming fungi. Parmelia lichens present a medium to large foliose thallus and they are distributed from the Artic to the Antartic continents, being more concentrated in temperate regions. According to its current description, the genus encompasses up to 41 different species and it is phylogenetically located within the Parmelioid clade (the largest group in the family). Interestingly, some of its species are among the most common epiphytic lichens in Europe such as Parmelia sulcata Taylor and Parmelia saxatilis (L.) Ach. The present work aims at providing a complete overview of the existing knowledge on the genus, from general concepts such as taxonomy and phylogeny, to their ecological relevance and biological interest for pharmaceutical uses. As reported, Parmelia lichens arise as valuable tools for biomonitoring environmental pollution due to their capacity to bioaccumulate metal elements and its response to acid rain. Moreover, they produce a wide array of specialized products/metabolites including depsides, depsidones, triterpenes and dibenzofurans, which have been suggested to exert promising pharmacological activities, mainly antimicrobial, antioxidant and cytotoxic activities. Herein, we discuss past and recent data regarding to the phytochemical characterization of more than 15 species. Even though the knowledge is still scarce in comparsion to other groups of organisms such as higher plants and other non-lichenized fungi. Reviewed works suggest that Parmelia lichens are worthy of further research for determining their actual possibilities as sources of bioactive compounds with potential therapeutic applications.Item HPLC isolation of antioxidant constituents from Xanthoparmelia spp(Journal of Pharmaceutical and Biomedical Analysis, 2010) Amo De Paz, Guillermo; Raggio Quílez, José; Gómez-Serranillos Cuadrado, María Pilar; Palomino Ruiz-Poveda, Olga María; González Burgos, Elena María; Carretero Accame, María Emilia; Crespo De Las Casas, Ana MaríaA chromatographic method is described for the purification and characterization of secondary lichen substances with biological activity. A simple reversed-phase high-performance liquid chromatography method with gradient elution has been developed that allows the determination and isolation of salazinic, usnic and stictic acids from lichen samples in a single run and the quantification of every acid in the tested extracts. The antioxidant activity of both the isolated compounds and the respective lichen belonging to Xanthoparmelia genus was determined by the Oxygen Radical Absorbance Capacity (ORAC) assay; their effect as free radical scavengers, effect on cell survival by the 3(4,5-dimethyltiazol-2-yl)-2,5-diphenyltetrazolium reduction assay and 2′,7′-dichlorofluorescin diacetate method were tested on U373 MG human astrocytome cell line. Both lichens extracts and all isolated compounds protected U373 MG cells from hydrogen peroxide-induced damage, suggesting that they could act as antioxidant agents in those neurodegenerative disorders associated with oxidative damage, such as Alzheimer's disease and Parkinson's disease.- Project number: 253
Item Implementación del modelo TPACK (Technological Pedagogical Content Knowledge) en un entorno virtual de enseñanza y aprendizaje de la Farmacocinética en su perspectiva clínica(2019) Gómez Oliver, Francisca; García García, Luis; González Burgos, Elena María; Pozo García, Miguel Angel; Moreno Montes, Eva María; Ureña Vacas, Isabel MaríaImplementación de metodologías de enseñanza-aprendizaje universitario activo e integrado gracias al desarrollo y aplicación de un TPACK (Technological Pedagogical Content Knowledge) en entornos virtuales de enseñanza y aprendizaje (EVEA). Este modelo se basa en la triada (pedagogía-contenido-tecnología) y constituye una alternativa eficaz a los métodos docentes clásicos. El objetivo es optimizar los procesos de enseñanza-aprendizaje de la farmacocinética clinica para estudiantes de la asignatura de Farmacología General. Item The Impact of CXCR4 Blockade on the Survival of Rat Brain Cortical Neurons(International Journal of Molecular Sciences, 2016) Merino Martín, José Joaquín; Garcimartín Álvarez, Alba; López-Oliva Muñoz, María Elvira; Benedí González, Juana María; González Burgos, Elena MaríaBackground: Chemokine receptor type 4 (CXCR4) plays a role in neuronal survival/cell repair and also contributes to the progression of cancer and neurodegenerative diseases. Chemokine ligand 12 (CXCL12) binds to CXCR4. In this study, we have investigated whether CXCR4 blockade by AMD3100 (a CXCR4 antagonist, member of bicyclam family) may affect neuronal survival in the absence of insult. Thus, we have measured the mitochondrial membrane potential (MMP), Bax and Bcl-2 protein translocation, and cytochrome c release in AMD3100-treated brain cortical neurons at 7 DIV (days in vitro). Methods: For this aim, AMD3100 (200 nM) was added to cortical neurons for 24 h, and several biomarkers like cell viability, reactive oxygen species (ROS) generation, lactate dehydrogenase (LDH) release, caspase-3/9 activity, proteins Bax and Bcl-2 translocation, and cytochrome c release were analyzed by immunoblot. Results: CXCR4 blockade by AMD3100 (200 nM, 24 h) induces mitochondrial hyperpolarization and increases caspase-3/9 hyperpolarization without affecting LDH release as compared to untreated controls. AMD3100 also increases cytochrome c release and promotes Bax translocation to the mitochondria, whereas it raises cytosolic Bcl-2 levels in brain cortical neurons. Conclusion: CXCR4 blockade induces cellular death via intrinsic apoptosis in rat brain cortical neurons in absence of insult.Item Estudio de la actividad neuroprotectora de diterpenos aislados del género sideritis(2013) González Burgos, Elena María; Gómez-Serranillos Cuadrado, María Pilar; Carretero Accame, María EmiliaLa excesiva producción de especies reactivas de oxígeno (ROS) en el cerebro puede llevar a una alteración de la homeostasis óxido-reducción intracelular (denominada estrés oxidativo) que trae consigo daño de las estructuras biológicas y muerte celular. Consistentes evidencias implican al estrés oxidativo en la fisiopatología de varias enfermedades neurodegenerativas incluidas la enfermedad de Alzheimer, la enfermedad de Parkinson, la enfermedad de Huntington y la Esclerosis Lateral Amiotrófica. El objetivo de este trabajo es el de evaluar el potencial antioxidante de seis diterpenos mayoritarios (andalusol, conchitriol, foliol, lagascatriol, linearol y sidol) aislados de especies del género Sideritis en dos modelos celulares, PC12 (neuronal) y U373-MG (astrocitoma), bajo condiciones de estrés oxidativo inducido por H2O2. El pretratamiento con los diterpenos objeto de estudio (concentraciones 5 y 10 M, 24 h) protege a ambos tipos celulares frente a los efectos dañinos derivados del estrés oxidativo y causados por H2O2 (concentraciones de 0,1 mM para las células PC12 y de 1 mM para las células U373-MG, 30 min). Estos diterpenos aumentan la viabilidad celular, atenúan los cambios morfológicos, inhiben la producción de ROS y la peroxidación lipídica, restablecen los cambios en los niveles de GSH y GSSG y aumentan la actividad y la expresión proteica de las principales enzimas antioxidantes. Además, estos diterpenos protegen a la mitocondria de los cambios derivados del estrés oxidativo; éstos disminuyen las alteraciones en la homeostasis del calcio (mitocondrial y citosólico), aumentan el potencial de membrana mitocondrial, atenúan los cambios en los niveles de ATP e inhiben la activación de la caspasa-3. [ABSTRACT] Excessive reactive oxygen species (ROS) production in the brain may disrupt intracellular redox homeostasis (called oxidative stress) that can cause damage of biological structures and lead to cell death. Evidence from other studies implicates oxidative stress in the physiopathology of several neurodegenerative diseases including Alzheimer's disease, Parkinson's disease, Huntington's disease and Amyotrophic Lateral Sclerosis. The objective of this study is to evaluate the antioxidant potential of six major diterpenes (andalusol, conchitriol, foliol, lagascatriol, linearol and sidol) isolated from species of the genus Sideritis in two cellular models, PC12 (neuronal) and U373-MG (astrocytoma) under oxidative stress conditions induced by H2O2. Pretreatments with diterpenes (concentrations 5 and 10 M, 24 h) protect both cellular types from the harmful effects of oxidative stress and caused by H2O2 (0.1 mM concentration for PC12 cells and 1 mM concentration for U373-MG cells, 30 min). These diterpenes increase cell viability, attenuate morphological changes, inhibit ROS production and lipid peroxidation, counteract GSH and GSSG levels and increase activity and protein expression of the main antioxidant enzymes. Moreover, these diterpenes protect mitochondria from oxidative stress-derived changes; these decrease calcium homeostasis alterations (mitochondrial and cytosolic), increase mitochondrial membrane potential, attenuate changes in ATP levels and inhibit caspase-3 activation.- Project number: 271
Item Creación de un SPOC (Small Private Open Course) colaborativo entre docentes y estudiantes para la mejora del aprendizaje de la Farmacología(2018) González Burgos, Elena María; García García, Luis; Gómez Serranillos Cuadrado, María Pilar; Garcia Broncano, Rosario; Sieteiglesias Mansilla, Víctor; Gómez Oliver, Francisca Item Lichens of Parmelioid Clade as Promising Multitarget Neuroprotective Agents(2019) Sieteiglesias Mansilla, Víctor; González Burgos, Elena María; Bermejo Bescos, María De La Paloma; Dulare Devi, Pradeep Divakar; Gómez-Serranillos Cuadrado, María PilarNeurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease are multifactorial disorders which are increasing in incidence and prevalence over the world without existing effective therapies. The search for new multitarget compounds is the latter therapeutic strategy to address these pathological conditions. Lichens have an important and unknown therapeutic value attributed to their unique secondary metabolites. The aim of this study is to evaluate for the first time the in vitro neuroprotective activities and molecular mechanisms underlying methanol extracts of lichens of the parmelioid clade and to characterize major bioactive secondary metabolites responsible for their pharmacological actions. Of the 15 parmelioid lichen species, our results showed that Parmotrema perlatum and Hypotrachyna formosana methanol extracts exhibited high antioxidant activity as evidenced in ORAC, DPPH, and FRAP assays. Then, SH-SY5Y cells were pretreated with methanol extracts (24 h) followed by Fenton reagent exposure (2 h). Pretreatments with these two more antioxidant methanol lichen extracts increased cell viability, reduced intracellular ROS, prevented oxidative stress biomarkers accumulation, and upregulated antioxidant enzyme (CAT, SOD, GR, and GPx) activity compared to Fenton reagent cells. The neuroprotective activity was much higher for H. formosana than for P. perlatum, even equal to or higher than Trolox (reference compound). Moreover, H. formosana extracts inhibited both AChE and BuChE activities in a concentration dependent manner, and P. perlatum only showed concentration dependent activity against AChE. Finally, chemical composition analysis using TLC and HPLC methods revealed that physodic acid, lividic acid, and lichexanthone are major secondary metabolites in H. formosana and stictic acid and constictic acid are in P. perlatum. These results demonstrated that P. perlatum and, specially, H. formosana are promising multitargeted neuroprotective agents due to their antioxidant and AChE and BuChE inhibition activities.Item Regulation of redox status in neuronal SH-SY5Y cells by blueberry (Vaccinium myrtillus L.) juice, cranberry (Vaccinium macrocarpon A.) juice and cyanidin(2018) Cásedas, Guillermo ; González Burgos, Elena María; Smith, Carine ; López, Víctor ; Gómez-Serranillos Cuadrado, María PilarBlueberry and cranberry are fruits with high polyphenol content, particularly anthocyanins. As cyanidin derivatives have been identified as one of the most representative polyphenols in berry juices, cyanidin has been designated for a better comparison and understanding of the potential neuroprotection of juices obtained from two Vaccinium species. Neuroblastoma SH-SY5Y cells were previously treated with different concentrations of lyophilized blueberry juice, cranberry juice or cyanidin for 24 h and oxidative stress was then generated with hydrogen peroxide (100 μM) for 30 min. Cytoprotective properties of cranberry juice, blueberry juice or cyanidin were evaluated using different methodologies such as mitochondrial activity (MTT), TBARS and ROS production, antioxidant enzymes (CAT, SOD) and antioxidant properties (ORAC, FRAP). Results indicated that blueberry and cranberry juices as well as cyanidin increased mitochondrial activity and reduced intracellular ROS production and lipid peroxidation induced by hydrogen peroxide. Furthermore, these berry juices and cyanidin upregulated the activity of the antioxidant enzymes catalase and superoxide dismutase. Finally, in vitro antioxidant capacities were confirmed by ORAC and FRAP assays demonstrating the potential of cyanidin and cyanidin-containing products for pharmaceutical or nutritional applications to prevent oxidative stress in neuronal cells.