Person:
González Burgos, Elena María

First Name

Elena María

Last Name

González Burgos

URI

https://hdl.handle.net/20.500.14352/77699

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Farmacia

Department

Farmacología, Farmacognosia y Botánica

Area

Farmacología

Identifiers

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Search Results

Now showing 1 - 10 of 35

Leveraging 3D-printed microfluidic micromixers for the continuous manufacture of melatonin loaded SNEDDS with enhanced antioxidant activity and skin permeability
(International Journal of Pharmaceutics, 2024) Ongoren, Baris; Aytug, Kara; Casettari, Luca; Tiboni, Mattia; Lalatsa, Aikaterini; Sanz Pérez, Amadeo; González Burgos, Elena María; Romero Martínez, Manuel Alejandro; Juberías, Antonio; Torrado Durán, Juan José; Serrano López, Dolores Remedios
Vesicants are chemical warfare agents (CWAs) capable of causing severe skin damage and systemic toxicity. Melatonin, known for its anti-inflammatory and antioxidant properties, can mitigate the effects of these agents. Self-nano-emulsifying drug delivery systems (SNEDDS) containing a high melatonin concentration (5 %, 50 mg/g) were optimized using a quality-by-design approach from biocompatible, non-irritant excipients with a particle size of about 100 nm. The melatonin-loaded SNEDDS showed a 43-fold greater permeability than a conventional melatonin cream. Chemical stability at ambient temperature (25 °C) was maintained for one year. The preparation of optimised melatonin-loaded SNEDDS using a simple mixing method was compared to microfluidic micromixers. Mixing was successfully achieved using a 3D-printed (fused deposition modeling or stereolithography) T-shaped toroidal microfluidic chip (with a channel geometry optimized by computational fluid dynamics), resulting in a scalable, continuous process for the first time with a substantial reduction in preparation time compared to other conventional mixing approaches. No statistically significant differences were observed in the key quality attributes, such as particle size and melatonin loading, between mixing method till kinetic equilibrium solubility is reached and mixing using the 3D-printed micromixers. This scalable, continuous, cost-effective approach improves the overall efficiency of SNEDDS production, reduces the cost of quality control for multiple batches, and demonstrates the potential of continuous microfluidic manufacture with readily customizable 3D-printed micromixers at points of care, such as military bases.
The Pharmacological Activity of Camellia sinensis (L.) Kuntze on Metabolic and Endocrine Disorders: A Systematic Review
(Biomolecules, 2020) Sánchez-Paniagua López, Marta; González Burgos, Elena María; Iglesias Peinado, Irene; Lozano Fernández, Rafael; Gómez-Serranillos Cuadrado, María Pilar
Tea made from Camellia sinensis leaves is one of the most consumed beverages worldwide. This systematic review aims to update Camellia sinensis pharmacological activity on metabolic and endocrine disorders. Inclusion criteria were preclinical and clinical studies of tea extracts and isolated compounds on osteoporosis, hypertension, diabetes, metabolic syndrome, hypercholesterolemia, and obesity written in English between 2014 and 2019 and published in Pubmed, Science Direct, and Scopus. From a total of 1384 studies, 80 reports met inclusion criteria. Most papers were published in 2015 (29.3%) and 2017 (20.6%), conducted in China (28.75%), US (12.5%), and South Korea (10%) and carried out with extracts (67.5%, especially green tea) and isolated compounds (41.25%, especially epigallocatechin gallate). Most pharmacological studies were in vitro and in vivo studies focused on diabetes and obesity. Clinical trials, although they have demonstrated promising results, are very limited. Future research should be aimed at providing more clinical evidence on less studied pathologies such as osteoporosis, hypertension, and metabolic syndrome. Given the close relationship among all endocrine disorders, it would be of interest to find a standard dose of tea or their bioactive constituents that would be beneficial for all of them.
Leveraging 3D-Printed Microfluidic Micromixers for the Continuous Manufacture of Melatonin Loaded SNEDDS with Enhanced Antioxidant Activity and Skin Permeability
(International Journal of Pharmaceutics, 2024) Ongoren, Baris; Kara, Aytug; Casettari, Luca; Tiboni, Mattia; Lalatsa, A.; Sanz-Perez, A.; González Burgos, Elena María; Romero Martínez, Manuel Alejandro; Juderías, Antonio; Torrado Durán, Juan José; Serrano López, Dolores Remedios
Vesicants are chemical warfare agents (CWAs) capable of causing severe skin damage and systemic toxicity. Melatonin, known for its anti-inflammatory and antioxidant properties, can mitigate the effects of these agents. Self-nano-emulsifying drug delivery systems (SNEDDS) containing a high melatonin concentration (5%, 50 mg/g) were optimized using a quality-by-design approach from biocompatible, non-irritant excipients with a particle size of about 100 nm. The melatonin-loaded SNEDDS showed a 43-fold greater permeability than a conventional melatonin cream. Chemical stability at ambient temperature (25oC) was maintained for one year. The preparation of optimised melatonin-loaded SNEDDS using a simple mixing method was compared to microfluidic micromixers. Mixing was successfully achieved using a 3D-printed (fused deposition modeling or stereolithography) T-shaped toroidal microfluidic chip (with a channel geometry optimized by computational fluid dynamics), resulting in a scalable, continuous process for the first time with a substantial reduction in preparation time compared to other conventional mixing approaches. No statistically significant differences were observed in the key quality attributes, such as particle size and melatonin loading, between mixing method till kinetic equilibrium solubility is reached and mixing using the 3D-printed micromixers. This scalable, continuous, cost-effective approach improves the overall efficiency of SNEDDS production, reduces the cost of quality control for multiple batches, and demonstrates the potential of continuous microfluidic manufacture with readily customizable 3D-printed micromixers at points of care, such as military bases.
Project number: 238
Implementación del aprendizaje-servicio en “Farmacología General” como estrategia para fomentar el conocimiento significativo.
(2020) Fernández Alfonso, María Soledad; Garcimartín Álvarez, Alba; González Burgos, Elena María; García García, Luis; Bedoya Del Olmo, Luis Miguel; Gómez Oliver, Francisca; Guerra Guirao, José Antonio; Manouchehri, Marjan; Manzano Lista, Francisco Javier; Ureña Vacas, Isabel María
Lichen depsides and tridepsides: progress in pharmacological approaches
(Journal of Fungi, 2023) Ureña Vacas, Isabel María; González Burgos, Elena María; Dulare Devi, Pradeep Divakar; Gómez-Serranillos Cuadrado, María Pilar
Depsides and tridepsides are secondary metabolites found in lichens. In the last 10 years, there has been a growing interest in the pharmacological activity of these compounds. This review aims to discuss the research findings related to the biological effects and mechanisms of action of lichen depsides and tridepsides. The most studied compound is atranorin, followed by gyrophoric acid, diffractaic acid, and lecanoric acid. Antioxidant, cytotoxic, and antimicrobial activities are among the most investigated activities, mainly in in vitro studies, with occasional in silico and in vivo studies. Clinical trials have not been conducted using depsides and tridepsides. Therefore, future research should focus on conducting more in vivo work and clinical trials, as well as on evaluating the other activities. Moreover, despite the significant increase in research work on the pharmacology of depsides and tridepsides, there are many of these compounds which have yet to be investigated (e.g., hiascic acid, lassalic acid, ovoic acid, crustinic acid, and hypothamnolic acid).
Self-assembling, supramolecular chemistry and pharmacology of amphotericin B: Poly-aggregates, oligomers and monomers
(Journal of Controlled Release, 2021) Raquel Fernández-García; Juan C. Muñoz-García; Matthew Wallace; Laszlo Fabian; González Burgos, Elena María; Gómez-Serranillos Cuadrado, María Pilar; Raposo González, Rafaela; Bolas Fernández, Francisco; Ballesteros Papantonakis, María De La Paloma; Anne Marie Healy; Yaroslav Z. Khimyak; Serrano López, Dolores Remedios
Antifungal drugs such as amphotericin B (AmB) interact with lipids and phospholipids located on fungal cell membranes to disrupt them and create pores, leading to cell apoptosis and therefore efficacy. At the same time, the interaction can also take place with cell components from mammalian cells, leading to toxicity. AmB was selected as a model antifungal drug due to the complexity of its supramolecular chemical structure which can self-assemble in three different aggregation states in aqueous media: monomer, oligomer (also known as dimer) and poly-aggregate. The interplay between AmB self-assembly and its efficacy or toxicity against fungal or mammalian cells is not yet fully understood. To the best of our knowledge, this is the first report that investigates the role of excipients in the supramolecular chemistry of AmB and the impact on its biological activity and toxicity. The monomeric state was obtained by complexation with cyclodextrins resulting in the most toxic state, which was attributed to the greater production of highly reactive oxygen species upon disruption of mammalian cell membranes, a less specific mechanism of action compared to the binding to the ergosterol located in fungal cell membranes. The interaction between AmB and sodium deoxycholate resulted in the oligomeric and polyaggregated forms which bound more selectively to the ergosterol of fungal cell membranes. NMR combined with XRD studies elucidated the interaction between drug and excipient to achieve the AmB aggregation states, and ultimately, their diffusivity across membranes. A linear correlation between particle size and the efficacy/toxicity ratio was established allowing to modulate the biological effect of the drug and hence, to improve pharmacological regimens. However, particle size is not the only factor modulating the biological response but also the equilibrium of each state which dictates the fraction of free monomeric form available. Tuning the aggregation state of AmB formulations is a promising strategy to trigger a more selective response against fungal cells and to reduce the toxicity in mammalian cells.
DNA-Based Authentication and Metabolomics Analysis of Medicinal Plants Samples by DNA Barcoding and Ultra-High-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (UHPLC-MS)
(Plants, 2020) Sánchez Gómez-Serranillos, Marta; González Burgos, Elena María; Dulare Devi, Pradeep Divakar; Gómez-Serranillos Cuadrado, María Pilar
There is growing interest for medicinal plants in the world drug market. Particularly, Matricaria recutita L., Valeriana officinalis L., Tilia spp., and Camellia sinensis (L.) Kuntze are some of the most consumed medicinal plants for treatment of minor health problems. Medicinal plants are seen as natural and safe; however, they can cause interactions and produce adverse reactions. Moreover, there is lack of consensus in medicinal plants regulation worldwide. DNA barcoding and UHPLC-MS technique are increasingly used to correctly identify medicinal plants and guarantee their quality and therapeutic safety. We analyzed 33 samples of valerian, linden, tea, and chamomile acquired in pharmacies, supermarkets, and herbal shops by DNA barcoding and UHPLC-MS. DNA barcoding, using matk as a barcode marker, revealed that CH1 sold as Camellia sinensis was Blepharocalyx tweediei, and sample TS2 sold as linden belong to Malvales. On the other hand, UHPLC-MS analysis revealed the presence of bioactive compounds (apigenin-7-glucoside, acetoxy valerenic acid, valerenic acid, epigallocatechin, and tiliroside). However, none of samples met minimum content of these active principles (except for valerenic acid in VF3) according to the European Medicines Agency (EMA) and Real Spanish Pharmacopeia. In conclusion, this study revealed the need to incorporate DNA barcoding and HPLC-MS techniques in quality controls of medicinal plants.
Estrategias de uso racional de medicamentos en personas mayores como mejora de la calidad de vida y de promoción del bienestar
(2021) Garcimartín Álvarez, Alba; García Aguilar, Ana; Gómez Oliver, Francisca; Guerra Guirao, José Antonio; Merino Martín, José Joaquín; García García, Luis; González Burgos, Elena María; Fernández Alfonso, María Soledad; Bocanegra De Juana, Aranzazu; Bedoya Del Olmo, Luis Miguel; Manzano Lista, Francisco Javier; Garcia Broncano, Rosario; Hernández Martín, Marina; Ureña Vacas, Isabel María
El proyecto “Estrategias de uso racional de medicamentos en personas mayores como mejora de la calidad de vida y de promoción del bienestar” se basa en la elaboración y diseño de estrategias de prevención y mejora en torno al cumplimiento terapéutico, el uso racional del medicamento, y la identificación temprana de reacciones adversas a medicamentos, entre otros problemas relacionados con el medicamento. Este proyecto es una continuación del anterior ApS UCM 2019 “Identificación de problemas relacionados con el medicamento y estrategias de mejora en personas mayores”. En esta ocasión, y por la pandemia por COVID-19, este ApS será virtual, desarrollándose diversos talleres en formato de vídeo para dar formación a las personas mayores en materia de medicamento y que se centrarán en el tratamiento de las distintas patologías de la meta 3.4. de los ODS 2030, con el fin de aumentar la calidad de vida y el bienestar social de este colectivo. Esta actividad se integra en la docencia de las asignaturas de Farmacología General, y Farmacología y Farmacoterapia del Grado en Farmacia y del Doble Grado de Farmacia y Nutrición como estrategia de aprendizaje para consolidar y profundizar lo aprendido en estas materias.
Project number: 249
Flipped Learning y Web App de Plantas Medicinales Nuevas estrategias educativas aplicadas al ámbito de la enseñanza en la asignatura de Farmacognosia y Fitoterapia
(2017) Gómez-Serranillos Cuadrado, María Pilar; González Burgos, Elena María; Fernández Moriano, Carlos; Iglesias Peinado, Irene; Gavilán García, Rosario Gloria; Garcia Broncano, Rosario
Identificación de problemas relacionados con el medicamento y estrategias de mejora en personas mayores
(2020) Fernández Alfonso, María Soledad; Cea Soriano, Trinidad Lucía; Astasio Arbiza, Paloma; Bedoya Del Olmo, Luis Miguel; Garcia Broncano, Rosario; García Ferrera, Alicia; García García, Luis; Garcimartín Álvarez, Alba; Gómez Oliver, Francisca; González Burgos, Elena María; Guerra Guirao, José Antonio; Manouchehri, Marjan; Manzano Lista, Francisco Javier; Ortega Molina, Soledad Paloma; Pulido Manzanero, José; Sánchez Ortíz, Sara; Santos Sancho, Juana María