Person:
Romero Martínez, Beatriz

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First Name
Beatriz
Last Name
Romero Martínez
URI
https://hdl.handle.net/20.500.14352/80458
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
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Author: Romero Martínez, Beatriz × Subject: 579 ×

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Now showing 1 - 3 of 3
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    Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection
    (Veterinary Microbiology, 2008) Álvarez Sánchez, Julio; Juan Ferré, Lucía De; Bezos Garrido, Javier; Romero Martínez, Beatriz; Sáez, Jose Luis; Reviriego Gordejo, F.J.; Briones Dieste, Víctor; Moreno Romo, Miguel Ángel; Mateos García, Ana Isabel; Domínguez Rodríguez, Lucas José; Aranaz Martín, Alicia
    Detection of infected animals is a key step in eradication programs of tuberculosis. Paratuberculosis infection has been demonstrated to compromise the specificity of the diagnostic tests. However, its effect on their sensitivity has not been clarified. In the present study, skin tests and the interferon-gamma (IFN-γ) assay were evaluated in a goat flock (n = 177) with a mixed tuberculosis–paratuberculosis infection in order to assess the possible effect of paratuberculosis on their sensitivity. Culture of mycobacteria was performed as the gold standard to determine the true infection status. All techniques showed lower sensitivities than previously described; the single intradermal tuberculin (SIT) test and the IFN-γ assay detected 71% (62.4–78.6, 95% C.I.) of the infected animals; the single intradermal cervical comparative tuberculin (SICCT) test detected only 42.7% (34.1–51.7, 95% C.I.) of infected animals. The highest level of sensitivity was obtained when SIT test and IFN-γ assay were combined in parallel (90.8%, 84.5–95.2, 95% C.I.). Sensitivities of the tests were also assessed by comparing animals suffering tuberculosis and animals with a mixed infection; tests were found to be more effective in the former group. Paratuberculosis seems to have a major effect in the sensitivity of the diagnostic tests under study, and therefore must be taken into account; in particular, the use of the SICCT test should be questioned when both tuberculosis and paratuberculosis are present.
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    Polyresistant Mycobacterium bovis Infection in Human and Sympatric Sheep, Spain, 2017–2018
    (Emerging Infectious Diseases, 2021) Romero Martínez, Beatriz; Pozo Piñol, Pilar; Pérez de Val, Bernat; Tórtola, Maria Teresa; Herrera León, Laura; Mercader, Irene; Sáez Lorente, José Luis; Domingo, Mariano; Vidal, Enric
    The main etiologic agent of tuberculosis (TB) in livestock is Mycobacterium bovis; human TB cases caused by M. bovis are rare. Analysis of a TB outbreak caused by polyresistant M. bovis involving a human and sympatric sheep in Spain suggests local circulation of drug-resistant M. bovis strains among livestock.
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    Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay
    (Veterinary Microbiology, 2009) Álvarez Sánchez, Julio; Juan Ferré, Lucía De; Bezos Garrido, Javier; Romero Martínez, Beatriz; Sáez, Jose Luis; Marqués, Sergio; Domínguez, Concepción; Mínguez, Olga; Fernández-Mardomingo, Baudilio; Mateos García, Ana Isabel; Domínguez Rodríguez, Lucas José; Aranaz Martín, Alicia
    Interferon-gamma (IFN-gamma) detection assay is being applied as an ancillary test to tuberculin tests in the diagnosis of bovine tuberculosis to detect the maximum number of infected animals. Among possible factors influencing the performance of tuberculosis-diagnostic tests, paratuberculosis, a widespread disease in Spain and other European countries, has been pointed out as a cause of false positive reactions. Still, its effect on the sensitivity of these tests in cattle has yet to be fully characterized. The impact of paratuberculosis in the apparent sensitivity of IFN-gamma assay was studied in a bullfighting cattle herd with a mixed tuberculosis-paratuberculosis infection, using culture of Mycobacterium bovis and Mycobacterium avium paratuberculosis as the gold standard to determine the infection status of every animal. A total of 218 animals were slaughtered and sampled for bacteriology after blood sampling. IFN-gamma assay showed a lower apparent sensitivity in animals with a mixed infection (50%) compared to all animals suffering tuberculosis (78.3%). This finding indicates that the presence of paratuberculosis in tuberculosis-infected herds could imply a serious impairment in the sensitivity of IFN-gamma detection test.