Person: Descalzo López, Ana Belén
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First Name
Ana Belén
Last Name
Descalzo López
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Orgánica
Area
Química Orgánica
Identifiers
5 results
Search Results
Now showing 1 - 5 of 5
- Project number: 134
Item Nueva metodología de aprendizaje basado en investigación en el Laboratorio de Química Orgánica II (Grado en Química, Doble Grado en Química y Bioquímica)(2022) Illescas Martínez, Beatriz; Martínez Ruiz, María Paloma; Rodríguez Yunta, María Josefa; Gómez Aspe, Rafael; Lora Maroto, Beatriz; Martínez del Campo, Teresa; Descalzo López, Ana Belén; Santos Barahona, Jose Manuel; Cembellín Santos, Sara; Cabrera González, Justo Enrique; Patino Alonso, Jennifer; Serrano Buitrago, Sergio; Villalonga Chico, Anabel; Mayol Hornero, Beatriz; Hernández-Cornejo Pérez, JavierEste proyecto tiene como objetivo llevar a cabo un cambio conceptual del Laboratorio de Química Orgánica II, introduciendo métodos del aprendizaje mediante indagación guiada (“inquiry-guided learning”). Se ha desarrollado un aprendizaje centrado en el estudiante, que requiere que los alumnos realicen sus propias hipótesis, planifiquen su experimento, reflexionen y tomen decisiones durante el desarrollo de cada sesión. Se han utilizado como estrategias principales: 1) Diseño de una práctica piloto basada en los conceptos del aprendizaje mediante indagación guiada. Dicha práctica es diferente para cada alumno, lo que ha supuesto contar con una reserva de 12-15 prácticas diferentes previamente ensayadas. 2) Diseño y elaboración del material de laboratorio necesario para transformar las prácticas ya existentes en formato expositivo en experimentos basados en indagación, planteando preguntas que los alumnos deben responder a lo largo del experimento, sin proporcionarles previamente los resultados esperados, sino promoviendo un aprendizaje activo que integra los nuevos conceptos inferidos a partir de un verdadero proceso de experimentación e investigación. 3) Promover la discusión científica de los resultados introduciendo al final de la sesión una puesta en común con un alto componente de evaluación entre iguales (“peer-assessment”). 4) Con el fin de tutorizar el papel del profesor en este modelo de aprendizaje, se ha desarrollado una guía de ayuda al profesor. 5) Por último, se han realizado encuestas de evaluación para recibir la retroalimentación necesaria de los profesores y alumnos que se han enfrentado a esta nueva metodología en nuestro Departamento. - Project number: 307
Item La “indagación guiada” en el Laboratorio de Química Orgánica II (Grado en Química, Doble Grado en Química y Bioquímica)(2023) Illescar Martínez, Beatriz María; Martínez Ruiz, Mª Paloma; Rodríguez Yunta, Mª Josefa; Gómez Aspe, Rafael; Lora Maroto, Beatriz; Luna Costales, Amparo; Descalzo López, Ana Belén; Vázquez Rodríguez, Henar; Santos Barahona, José; Cembellín Santos, Sara; Cabrera González, Justo Enrique; Patino Alonso, Jennifer; Serrano Buitrago, Sergio; Foronda Sainz, Román; Nieto Ortiz, Gema Isabel; García Matesanz, Diego; López Zazo, Ruth; García Escobar, Mª Eugenia; Calderón Saturio, DeliaEl objetivo del proyecto es introducir la indagación guiada en el Laboratorio de Química Orgánica II. Supone la modificación de las prácticas, elaboración del material docente y creación de una sesión de búsqueda bibliográfica online. Item Eu(III)-Templated molecularly imprinted polymer used as a luminescent sensor for the determination of tenuazonic acid mycotoxin in food samples(Sensors and Actuators B: Chemical, 2021) Rico Yuste, Alberto; Abouhany, Rahma; Urraca Ruiz, Javier; Descalzo López, Ana Belén; Orellana Moraleda, Guillermo; Moreno Bondi, María CruzTenuazonic acid (TeA) is a common mycotoxin produced by Alternaria species found in various foodstuffs. Herein, we describe the preparation of porous molecularly imprinted polymer (MIP) microspheres doped with Eu(III), for selective fast analysis of TeA in rice extracts. The TeA template, bearing a β-diketone moiety, is a good Eu(III)-chelating antenna ligand that can be selectively captured in the MIP cavities and detected by observing the “turned-on” sensitized luminescence of the Eu(III) ions at 615 nm (λex 337 nm). A MIP library was prepared at small scale using diethyl allylmalonate (DEAM) or allyl acetoacetate as functional monomers, mixed in different mole ratios with the template and ethylene glycol dimethacrylate (EDMA) as cross-linker. The screening allowed selection of poly(DEAM-co-EDMA) with Eu(III) as luminogenic metal center for the sensor development. Under the optimized conditions, a linear response to TeA in the 1.7–20 μg mL−1 range, with a detection limit of 0.5 μg mL−1, was obtained. The Eu(III)-doped MIP for TeA showed very little or no cross-recognition of other mycotoxins present in foodstuff such as alternariol, β-zearalenol and cyclopiazonic acid, even containing the same recognition moiety. The MIP-based optosensor was successfully applied to the analysis of rice extracts spiked with TeA, and the results were confirmed by HPLC-DAD.Item Interaction of a 1,3-Dicarbonyl Toxin with Ru(II)-Biimidazole Complexes for Luminescence Sensing: A Spectroscopic and Photochemical Experimental Study Rationalized by Time-Dependent Density Functional Theory Calculations(Inorganic Chemistry, 2022) Quílez Alburquerque, José; García-Iriepa, Cristina; Marazzi, Marco; Descalzo López, Ana Belén; Orellana Moraleda, GuillermoA family of ruthenium(II) complexes containing one 2,2′-biimidazole (bim) ligand and two polypyridyl (NN) ligands has been prepared and their photophysical and photochemical features have been tested in the presence of tenuazonic acid (TeA), a widespread food and feed mycotoxin of current concern. While not tested in in vivo studies, TeA and other secondary metabolites of Alternaria fungi are suspected to exert adverse effects on the human health, so sensors and rapid analytical procedures are required. It is well-known that 1,3-dicarbonyl compounds such as TeA are relatively easy to deprotonate (the pKa of TeA is 3.5), yielding an enolate anion stabilized by resonance. The chelating and hydrogen-donor features of bim allow simultaneous binding to the metal core and to the target β-diketonate delocalized anion. Such a binding induces changes in the blue absorption (40 nm bathochromic shift), red luminescence intensity (>75% quenching), and triplet lifetime (0.2 μs decrease) of the Ru(NN)2(bim)2+ luminophore. Moreover, we have computationally rationalized, by time-dependent density functional theory, the structure of the different adducts of Ru–bim complexes with TeA and the electronic nature of the spectral absorption bands and their change upon the addition of TeA.Item On the Aggregation Behaviour and Spectroscopic Properties of Alkylated and Annelated Boron-Dipyrromethene (BODIPY) Dyes in Aqueous Solution(ChemPhotoChem, 2020) Descalzo López, Ana Belén; Ashokkumar, Pichandi; Shen, Zhen; Rurack, KnutThe tendency of boron-dipyrromethene (BODIPY) dyes to associate in water is well known, and usually a cause for inferior fluorescence properties. Synthetic efforts to chemically improve BODIPYs’ water solubility and minimize this problem have been numerous in the past. However, a deeper understanding of the phenomena responsible for fluorescence quenching is still required. Commonly, the spectroscopic behaviour in aqueous media has been attributed to aggregate or excimer formation, with such works often centring on a single BODIPY family. Herein, we provide an integrating discussion including very diverse types of BODIPY dyes. Our studies revealed that even subtle structural changes can distinctly affect the association behaviour of the fluorophores in water, involving different photophysical processes. The palette of behaviour found ranges from unperturbed emission, to the formation of H or J aggregates and excimers, to the involvement of tightly bound, pre-formed excimers. These results are a first step to a more generalized understanding of spectroscopic properties vs. structure, facilitating future molecular design of BODIPYs, especially as probes for biological applications.