Person:
Vivanco Martínez, Fernando

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First Name
Fernando
Last Name
Vivanco Martínez
URI
https://hdl.handle.net/20.500.14352/84150
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Area
Bioquímica y Biología Molecular
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Author: Bartolomé, Borja × Subject: 577.1 ×

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    Allergy to human seminal fluid: Cross-reactivity with dog dander
    (The Journal of Allergy and Clinical Immunology, 2008) Basagaña, María; Bartolomé, Borja; Pastor Vargas, Carlos; Torres, Ferrán; Alonso, Rosario; Vivanco Martínez, Fernando; Cisteró-Bahíma, Anna
    Background: Human seminal plasma (HSP) allergy is uncommon, with symptoms ranging from vulvovaginal pruritus to life-threatening anaphylaxis. Although several seminal plasma allergens have been reported and their molecular masses have been estimated to range between 12 and 75 kd, the prostate-specific antigen (PSA) has recently been identified as a causative allergen. Given that in a large number of cases symptoms appeared during or after the first intercourse, a cross-reactivity phenomenon might be implicated. Objective: We sought to assess the presence of IgE cross-reactivity among proteins from dog epithelium and HSP and to attempt to identify the allergens involved. Methods: Forty-one patients with dog epithelium allergy were selected. One of them experienced anaphylaxis in contact with her husband's seminal plasma. Skin prick tests, serum specific IgE measurements, SDS-PAGE immunoblotting, and inhibition tests were performed to study the pattern of IgE-binding proteins and the potential cross-reactivity between HSP and dog epithelium. Mass spectrometry was carried out to identify the protein involved in allergy reactions. Results: Twenty-four percent of the sera from patients with dog epithelium allergy recognized an IgE-binding band of 28 kd in HSP immunoblotting. Mass spectrometry identified this band as the PSA. SDS-PAGE immunoblotting-inhibition showed a complete IgE-binding inhibition when sera from these patients were preincubated with dog dander extract. Conclusions: IgE cross-reactivity among proteins from dog dander and human PSA is demonstrated.