Person: Vivanco Martínez, Fernando
Loading...
First Name
Fernando
Last Name
Vivanco Martínez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Area
Bioquímica y Biología Molecular
Identifiers
17 results
Search Results
Now showing 1 - 10 of 17
Item Identification of thaumatin‐like protein and aspartyl protease as new major allergens in lettuce (Lactuca sativa).(Molecular nutrition and food research, 2013) Muñoz‐García, Esther; Luengo‐Sánchez, Olga; Haroun‐Díaz, Elisa; Sanz Maroto, Aroa; Palacín, Arancha; Díaz –Perales, Araceli; de las Heras Gozalo, Manuel; Labrador‐Horrillo, Moisés; Vivanco Martínez, Fernando; Cuesta‐Herranz, Javier; Pastor Vargas, CarlosScope: Today, about 2-8% of the population of Western countries exhibits some type of food allergy whose impact ranges from localized symptoms confined to the oral mucosa to severe anaphylactic reactions. Consumed worldwide, lettuce is a Compositae family vegetable that can elicit allergic reactions. To date, however, only one lipid transfer protein has been described in allergic reaction to lettuce. The aim of this study was to identify potential new allergens involved in lettuce allergy. Methods and results: Sera from 42 Spanish lettuce-allergic patients were obtained from patients recruited at the outpatient clinic. IgE-binding proteins were detected by SDS-PAGE and immunoblotting. Molecular characterization of IgE-binding bands was performed by MS. Thaumatin was purified using the Agilent 3100 OFFGEL system. The IgE-binding bands recognized in the sera of more than 50% of patients were identified as lipid transfer protein (9 kDa), a thaumatin-like protein (26 kDa), and an aspartyl protease (35 and 45 kDa). ELISA inhibition studies were performed to confirm the IgE reactivity of the purified allergen. Conclusion: Two new major lettuce allergens-a thaumatin-like protein and an aspartyl protease-have been identified and characterized. These allergens may be used to improve both diagnosis and treatment of lettuce-allergic patients.Item Occupational rhinitis due to inhalation of chicken meat protein.(J Investig Allergol Clin Immunol, 2014) Lobera Labairu, T; González Mahave, I; Del Pozo Gil, M D; Venturini Díaz, M; Blasco Sarramián, A; Pastor Vargas, Carlos; Vivanco Martínez, Fernando; Bartolomé Zavala, BorjaItem Detection of major food allergens in amniotic fluid: initial allergenic encounter during pregnancy(Pediatr Allergy Immunol, 2016) Pastor Vargas, Carlos; Aroa S. Maroto; Araceli Díaz‐Perales; Villalba Díaz, María Teresa; Vanesa Esteban; Marta Ruiz‐Ramos; Marta Rodriguez de Alba; Vivanco Martínez, Fernando; Javier Cuesta‐HerranzBackground: Ingestion of food allergens present in maternal milk during breastfeeding has been hypothesized as a gateway to sensitization to food; however, this process could develop during pregnancy, as the maternal-fetal interface develops a Th2- and Treg-mediated environment to protect the fetus. We hypothesized that in these surroundings, unborn children are exposed to food allergens contained in the mother's diet, possibly giving rise to first sensitization. Methods: The presence of allergens in utero was studied by analyzing amniotic fluid (AF) samples in two different stages of pregnancy: at 15-20 weeks and after delivery at term. An antibody microarray was developed to test for the most common food allergens. The array detects the presence of ten allergens from milk, fruit, egg, fish, nuts, and wheat. Results: AF from 20 pregnant women was collected: eight after delivery at term and 12 from women who underwent diagnostic amniocentesis between weeks 15 and 20 of gestation. The presence of allergens was detected in all samples. Samples from amniocentesis had a higher allergen concentration than samples after delivery at term. Conclusions: We demonstrated the presence of intact major food allergens in AF samples. This early contact could explain subsequent sensitization to foods never eaten before.Item Angler fish is not an alternative in allergy to multiple fish species(ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2014) Julián Azofra; Aroa Sanz Maroto; Vivanco Martínez, Fernando; Pastor Vargas, CarlosFish allergy is one of the most frequent food allergies. 1 Its prevalence is highest is those regions where consumption is greatest. Spain has one of the highest fish intakes per inhabitant and, as a result, fish allergy is a serious health problem.Item Allergy to crayfish(J Investig Allergol Clin Immunol, 2011) Gonzalez de Olano, David; Pastor Vargas, Carlos; Gandolfo-Cano, Mar; González-Mancebo, Eloina; Meléndez-Baltanás, Amaya; Morales-Barrios, Pilar; Pérez-Gordo, Marina; Vivanco Martínez, Fernando; Bartolomé, BorjaItem Molecular and Immunological Characterization of the First Allergenic Lipocalin in Hamster: the major allergen from Siberian hamster (Phodopus sungorus)(Journal of Biological Chemistry, 2014) José Alberto Torres; Manuel de las Heras; Aroa Sanz Maroto; Vivanco Martínez, Fernando; Joaquín Sastre; Pastor Vargas, CarlosThe most frequent pet allergy is to cat and dog, but in recent years, it has become increasingly popular to have other pets, and the risk of exposure to new allergens is more prevalent. The list of new pets includes hamsters, and one of the most popular hamsters is the Siberian hamster (Phodopus sungorus). The aim of this study was the characterization and cloning of the major allergen from this hamster. The study of its allergenicity and cross-reactivity could improve the specific diagnosis and treatment for hamster-allergic patients. Thirteen Siberian hamster-allergic patients were recruited at the outpatient clinic. Protein extracts were prepared from the hair, urine, and salivary glands of four hamster species (European, golden, Siberian, and Roborovski). IgE-binding proteins were detected by immunoblotting and identified by mass spectrometry. The recombinant protein was produced in Escherichia coli and then purified by metal chelate affinity chromatography. The allergenic properties of the recombinant protein were tested by ELISA and immunoblotting, and biological activity was tested according to capacity for basophil activation. Three IgE-binding proteins were identified in extracts obtained from Siberian hamster hair, urine, and salivary glands. All proteins corresponded to the same protein, which was identified as a lipocalin. This lipocalin had no cross-reactivity with common and golden hamsters. The recombinant allergen was cloned and purified, showing similar IgE reactivity in vitro to Siberian hamster protein extracts. Also, the recombinant allergen was capable of producing biological activation in vivo. The major Siberian hamster allergen was cloned, and allergenic properties were characterized, providing a new tool for specific diagnosis of allergy to Siberian hamster.Item Phosphorylation reduces the allergenicity of cow casein in children with selective allergy to goat and sheep milk.(J Investig Allergol Clin Immunol, 2011) Cases, Barbara; García-Ara, Carmen; Boyano, Maria Teresa; Pérez-Gordo, Marina; Pedrosa, Maria; Vivanco Martínez, Fernando; Quirce, Santiago; Pastor Vargas, CarlosThis study aimed to characterize the role of phosphorylation of caseins in selective allergy to goat milk (GM) and sheep milk (SM) in patients with good tolerance to cow milk (CM). We performed skin prick tests with milk and caseins from CM, GM, and SM and immunoblotting and specific immunoglobulin (Ig) E determinations with milk and casein from cow and GM and SM. Sensitization to milk and caseins from goat and sheep was demonstrated in all 3 patients by skin tests, determination of specific IgE, or both. Immunoblotting confirmed that GM/SM proteins but not CM proteins were involved in the allergic symptoms. IgE reacted with several protein bands from the caseins and milk extracts of both sheep and goat. Phosphorylation was involved in the different allergenicity of CM caseins. We report the implication of phosphorylation in the allergenicity of caseins involved in selective allergy to GM and SM.Item New allergen involved in a case of allergy to Solea solea, common sole(ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2010) Pérez-Gordo, Marina; Pastor Vargas, Carlos; Cases, Bárbara; De Las Heras, Manuel; Sanz, Aroa; Vivanco Martínez, Fernando; Cuesta-Herranz, JavierItem IDENTIFICATION OF A NOVEL 17-kDa PROTEIN AS A FERRET ALLERGEN(ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2009) González de Olano, David; Pastor Vargas, Carlos; Cases Ortega, Bárbara; Perez-Gordo, Marina; Moral Darde, Verónica; Vivanco Martínez, Fernando; Bartolomé, BorjaDomestic ferret (Mustela putorius furo) is a mammal from the Mustelidae family. It is the third most common uncaged pet in North America after dogs and cats. In Europe, its popularity is progressively increasing, and it is also becoming a common pet. The role of cats and dogs as a cause of allergy is well known. However, ferrets are not so widely studied as a source of relevant allergens.Item Allergen Characterization of Chia Seeds (Salvia hispanica), a new allergenic food(Journal of Investigational Allergology and Clinical Immunology, 2015) García Jiménez S; Pastor Vargas, Carlos; de las Heras M; Sanz Maroto A; Vivanco Martínez, Fernando; Sastre JSalvia hispanica, known also by its popular name, chia, is a plant of the Lamiaceae family. The plant is considered a pseudocereal and has a high oil and protein content. Mayans and Aztecs used it as a medicinal product and food supplement for added endurance. Its nutraceutical properties are due to high content in dietary fiber, natural antioxidants, and unsaturated fatty acids (60% α-linolenic acid). The protein content of chia is higher than that of most traditional grains.