Person: Pérez Carrasco, María Jesús
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First Name
María Jesús
Last Name
Pérez Carrasco
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
Identifiers
3 results
Search Results
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Item Effects of Light‐emitting Diode Radiations on Human Retinal Pigment Epithelial Cells in vitro(Photochemistry and Photobiology, 2012) Chamorro, Eva; Muñoz de Luna, Javier; Bonnin Arias, Cristina Natalia; Pérez Carrasco, María Jesús; Vázquez Molini, Daniel; Sánchez Ramos, CeliaHuman visual system is exposed to high levels of natural and artificial lights of different spectra and intensities along lifetime. Light-emitting diodes (LEDs) are the basic lighting components in screens of PCs, phones and TV sets; hence it is so important to know the implications of LED radiations on the human visual system. The aim of this study was to investigate the effect of LEDs radiations on human retinal pigment epithelial cells (HRPEpiC). They were exposed to three light-darkness (12 h/12 h) cycles, using blue-468 nm, green-525 nm, red-616 nm and white light. Cellular viability of HRPEpiC was evaluated by labeling all nuclei with DAPI; Production of reactive oxygen species (ROS) was determined by H2DCFDA staining; mitochondrial membrane potential was quantified by TMRM staining; DNA damage was determined by H2AX histone activation, and apoptosis was evaluated by caspases-3,-7 activation. It is shown that LED radiations decrease 75-99% cellular viability, and increase 66-89% cellular apoptosis. They also increase ROS production and DNA damage. Fluorescence intensity of apoptosis was 3.7% in nonirradiated cells and 88.8%, 86.1%, 83.9% and 65.5% in cells exposed to white, blue, green or red light, respectively. This study indicates three light-darkness (12 h/12 h) cycles of exposure to LED lighting affect in vitro HRPEpiC.Item Controlled pupilar miosis to improve mesopic visual function in drivers over 40 years old.(2012) Navarro Valls, J; Ramírez Mercado, G.; Pérez Carrasco, María Jesús; Lobato Rincón, Luis Lucio; Chamorro, E.; Sánchez Ramos, CeliaPurpose: To evaluate the influence of a difuse illumination device located outside the field of vision that provokes a controlled pupillar miosis, on the visual acuity and contrast sensitivity in drivers tested in mesopic conditions Methods: Cross‐sectional prospective observational study in which visual Acuity 100% and 20% contrast by ETDRS Test (VA100 and VA20) and Contrast Sensitivity by Pelli‐ Robson Test (CS) were evaluated in 39 drivers individuals under 40 years old and 30 drivers over 40. The study was carried out in a simulated setting for night driving with and without the interposition of the difuse illumination device Results: In individuals aged over 40 years, an statistically significant improvement of visual function was exhibit. Baseline visual acuity and contrast sensitivity scores were: VA100=0.02±0.18logMAR, VA20=0.21±0.2logMAR, and CS=0.62±0.32, whereas values for the interposition of difuse illumination device were: VA100=‐0.03±0.14logMAR(p=0.02), VA20=0.19±0.17logMAR(p=0.3), and CS=0.76±0.33(p=0.0003). On the other hand, in drivers aged under 40, statistically significance was not found. In this condition, baseline visual function was: VA100=‐0.1±0.11logMAR, VA20=0.13±0.19logMAR, and CS=1.06±0.2, whereas the interposition of the mentioned system showed the following values: VA100=0.01±0.09logMAR(p=0.9), VA20=0.09±0.14logMAR(p=0.3), and CS=1.08±0.16(p=0.6) Conclusion :Using difuse illumination device in the interior of the vehicle, provides significant improvement in both high contrast visual acuity and contrast sensitivity in the group older than 40 yearsItem Acid-Sensing ion channels 2 and 4 (Asic 2 Saic 4) are regulated by light in the zebrafish retina(2012) Sánchez Ramos, Celia; Germaná, A.; Bonnin Arias, Cristina Natalia; Chamorro Gutiérrez, E.; Navarro Valls, J.J.; García Ortega, M.; Pérez Carrasco, María Jesús; Vega, J.A.Purpose: Acid-sensing ion channels (ASICs) are H+- gated cation channels that monitor deviations from the physiological values of extracellular pH. ASIC genes in zebrafish (zASICs) are expressed in the central nervous system and the retina. pH variations in the retina are thought to be involved in the fine-tunning of visual perception and in the adaptation of the retinal responses to different light-induced retinal degeneration. This study examines the effects of continuous light or darkness exposure in the mRNA levels and cell distribution of ASIC2 and ASIC4 in the retina of adult zebrafish.