Person: Vallet Regí, María Dulce Nombre
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First Name
María Dulce Nombre
Last Name
Vallet Regí
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Química en Ciencias Farmacéuticas
Area
Química Inorgánica
Identifiers
43 results
Search Results
Now showing 1 - 10 of 43
Item From Proof-of-Concept Material to PEGylated and Modularly Targeted Ultrasound-Responsive Mesoporous Silica Nanoparticles(Journal of Materials Chemistry B, 2018) Paris, J.L.; Villaverde, Gonzalo; Cabañas Criado, María Victoria; Manzano García, Miguel; Vallet Regí, María Dulce NombreIn this work we present the synthesis, characterization and in vitro biological evaluation of PEGylated and actively-targeted ultrasound-responsive hybrid mesoporous silica nanoparticles. This work covers the development of the chemical strategies necessary to afford a modular nanocarrier starting from a proof-of-concept material presented in previous work. This functional ultrasound-responsive material can be adapted to different specific pathological conditions by carefully choosing the appropriate targeting moieties. The new ultrasound responsive material is able to target HeLa cells when conjugated with biotin or an RGD peptide. Ultrasound-responsive cytotoxicity towards cancer cells of doxorubicinloaded nanoparticles is demonstrated in an in vitro cytotoxicity assay.Item Tuning dual-drug release from composite scaffolds for bone 2 regeneration(International Journal of Pharmaceutics, 2015) Paris, J.L.; Román, M.; Cabañas Criado, María Victoria; Vallet Regí, María Dulce Nombre; Manzano García, MiguelThis work presents the tuning of drug-loaded scaffolds for bone regeneration as dual-drug delivery systems. Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (antiinflammatory drug) were successfully incorporated in a controlled mannerinto three dimensional designed porous scaffolds of apatite/agarose composite. A high-performance liquid chromatography method was optimized to separate and simultaneously quantify the two drugs released from the dualdrug codelivery system. The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the antiosteoporotic drug showed sustained release behaviour (important to promote bone regeneration). Since ibuprofen release was faster than desired, this drug was encapsulated in chitosan spheres which were then incorporated into the scaffolds, obtaining a release profile suitable for clinical application. The results obtained open the possibility to simultaneously incorporate two or more drugs to an osseous implant in a controlled way improving it for bone healing application.Item Nanoparticles to Knockdown Osteoporosis-Related Gene and Promote Osteogenic Markers Expression for Osteoporosis Treatment(ACS Nano, 2019) Mora Raimundo, Patricia; Lozano Borregón, Daniel; Manzano García, Miguel; Vallet Regí, María Dulce NombreOsteoporosis is the most common disease involving bone degeneration. Current clinical treatments are not able to offer a satisfying curative effect, so the development of effective treatments is desired. Gene silencing through siRNA delivery has gained great attention as a potential treatment in bone diseases. SOST gene inhibits the Wnt signaling pathway reducing osteoblast differentiation. Consequently, silencing SOST gene with a specific siRNA could be a potential option to treat osteoporosis. Generally, siRNAs have very short half-life and poor transfection capacity, so an effective carrier is needed. In particular, mesoporous silica nanoparticles (MSNs) have attracted great attention for intracellular delivery of nucleic acids. We took advantage of their high loading capacity to further load the pores with osteostatin, an osteogenic peptide. In this study we developed a system based on MSNs coated with poly(ethylenimine), which can effectively deliver SOST siRNA and osteostatin inside cells, with the consequent augmentation of osteogenic markers with a synergistic effect. This established the potential utility of MSNs to co-deliver both biomolecules to promote bone formation, being a potential alternative to treat osteoporosis.Item Electrical stimuli to increase cell proliferation on carbon nanotubes/mesoporous silica composites for drug delivery(Biomedical Materials Research - Part A, 2013) Vila Juárez, Mercedes; Cicuéndez Maroto, Mónica; Sánchez-Marcos, Jorge; Fal-Miyar, Vanesa; Manzano García, Miguel; Prieto, Carlos; Vallet Regí, María Dulce Nombre; Prieto Martín, CarlosThe development of smart materials as bone implants is nowadays a challenging task to optimize their fast osteointegration. Nevertheless, no attempts have been done in joining the possibility of using electrical stimulation and drug delivery together in a material intended for bone tissue engineering. Moreover, the use of this synergy to induce bone healing is still limited until novel drug reservoirs material formulations allow an efficient applicability of the electrical stimuli. Herein, we present the biological response of osteoblasts cells, cultured over carbon nanotubes–mesoporous silica composites while exposed to external electrical stimulus. Moreover, its ability to function as drug delivery systems is also demonstrated. Bone cell metabolism was stimulated and mitochondrial activity was increased up to seven times in the presence of these composites under electrical stimulus, suggesting their potential application in bone regeneration processesItem Editorial Chemical Engineering Journal(2018) Vallet Regí, María Dulce Nombre; Salinas Sánchez, Antonio J.; Baeza, Alejandro; Manzano García, MiguelItem Non-replicative antibiotic resistance-free DNA vaccine encoding S and N proteins induces full protection in mice against SARS-CoV-2(Frontiers in Immunology, 2022) Alcolea Alcolea, Pedro José; Larraga, Jaime; Rodríguez-Martín, Daniel; Alonso, Ana; Loayza, Francisco; Rojas, José M.; Ruiz García, Silvia; Louloudes Lázaro, Andrés; Carlón, Ana B.; Sánchez Cordón, Pedro José; Nogales Altozano, Pablo; Redondo, Natalia; Manzano García, Miguel; Lozano Borregón, Daniel; Palomero, Jesús; Montoya, María; Vallet Regí, María Dulce Nombre; Martín García, Verónica; Sevilla, Noemí; Larraga, VicenteSARS-CoV-2 vaccines currently in use have contributed to controlling the COVID-19 pandemic. Notwithstanding, the high mutation rate, fundamentally in the spike glycoprotein (S), is causing the emergence of new variants. Solely utilizing this antigen is a drawback that may reduce the efficacy of these vaccines. Herein we pre ent a DNA vaccine candidate that contains the genes encoding the S and the nucleocapsid (N) proteins implemented into the nonreplicative mammalian expression plasmid vector, pPAL. This plasmid lacks antibiotic resistance genes and contains an alternative selectable marker for production. The S gene sequence was modified to avoid furin cleavage (Sfs). Potent humoral and cellular immune responses were observed in C57BL/6J mice vaccinated with pPAL-Sfs + pPAL-N following a prime/boost regimen by the intramuscular route applying in vivo electroporation. The immunogen fully protected K18-hACE2 mice against a lethal dose (105 PFU) of SARS-CoV-2. Viral replication was completely controlled in the lungs, brain, and heart of vaccinated mice. Therefore, pPAL-Sfs + pPAL-N is a promising DNA vaccine candidate for protection from COVID-19.Item Engineering Mesoporous Silica Nanoparticles for Drug Delivery: where are we after two decades?(Chemical Society Reviews, 2022) Vallet Regí, María Dulce Nombre; Schüth, Ferdi; Lozano Borregón, Daniel; Colilla Nieto, Montserrat; Manzano García, MiguelThe present review details a chronological description of the events that took place during the development of mesoporous materials, their different synthetic routes and their use as drug delivery systems. The outstanding textural properties of these materials quickly inspired their translation to the nanoscale dimension leading to mesoporous silica nanoparticles (MSNs). The different aspects of introducing pharmaceutical agents into the pores of these nanocarriers, together with their possible biodistribution and clearance routes, would be here described. The development of smart nanocarriers able to release high local concentration of the therapeutic cargo on-demand after the application of certain stimuli would be here reviewed, together with the ability of delivering the therapeutic cargo to precise locations in the body. The huge progress in the design and development of MSNs for biomedical applications, including the potential treatment of different diseases, during the last 20 years will be here collected, together with the required work that still needs to be done to achieve the clinical translation of these materials. This review was conceived to stand out from past reports since aims to tell the story of the development of mesoporous materials and their use as drug delivery systems by some of the story makers, who could be considered to be among the pioneers in this area.- Project number: 15
Item Aprendizaje autónomo del Laboratorio de Química Inorgánica mediante el uso de TICs(2016) Román Zaragoza, Jesús; Cabañas Criado, María Victoria; Doadrio Villarejo, Juan Carlos; Doadrio Villarejo, Antonio Luis; García Diz, Luis; García Fontecha, Ana; Gutiérrez Ríos, María Teresa; Lozano Fernández, Rafael; Manzano García, Miguel; Martínez Alonso, África; Peña López, Juan; Sánchez Salcedo, Sandra; Vallet Regí, María Dulce NombreSe ha creado un Entorno Virtual de Enseñanza y Aprendizaje (EVEA) circunscrito a la realización de prácticas en el Laboratorio de Química Inorgánica orientado a incrementar el grado de interacción entre el alumno con algún tipo de dificultad auditiva o dificultad idiomática y el profesor o el resto de sus compañeros mediante el uso conjunto del material elaborado y el uso de sistemas basados en redes sociales, mensajerías. Item Recent advances in mesoporous silica nanoparticles for antitumor therapy: our contribution(Biomaterials Science, 2016) Baeza García, Alejandro; Manzano García, Miguel; Colilla Nieto, Montserrat; Vallet Regí, María Dulce NombreSince 2001, when our research group proposed for the first time MCM-41 as a drug release system, the scientific community has demonstrated a growing interest in mesoporous silica nanoparticles (MSNs) for their revolutionary potential in nanomedicine. Among the diverse pathologies that can be treated with MSNs, cancer has received increasing attention. MSNs can be loaded with large amounts of therapeutic cargoes and once intravenously administrated preferentially accumulate in solid tumours via the enhanced permeation and retention (EPR) effect. Herein we report the recent developments achieved by our research group as a pioneer in this field, highlighting: the design of sophisticated MSNs as stimuli-responsive drug delivery systems to release the entrapped cargo upon exposure to a given stimulus while preventing the premature release of highly cytotoxic drugs before reaching the target; transporting non-toxic prodrugs and the enzyme responsible for its conversion into cytotoxic agents into the same MSNs; improving the selectivity and cellular uptake by cancer cells by active targeting of MSNs; increasing the penetration of MSNs within the tumour mass, which is one of the major challenges in the use of NPs to treat solid tumours.Item Mesoporous Silica Nanoparticles for the Treatment of Complex Bone Diseases: Bone Cancer, Bone Infection and Osteoporosis(Pharmaceutics, 2020) Gisbert Garzarán, Miguel; Manzano García, Miguel; Vallet Regí, María Dulce NombreBone diseases, such as bone cancer, bone infection and osteoporosis, constitute a major issue for modern societies as a consequence of their progressive ageing. Even though these pathologies can be currently treated in the clinic, some of those treatments present drawbacks that may lead to severe complications. For instance, chemotherapy lacks of great tumor tissue selectivity, affecting healthy and diseased tissues. In addition, the inappropriate use of antimicrobials is leading to the appearance of drug-resistance bacteria and persistent biofilms, rendering current antibiotics useless. Furthermore, current antiosteoporotic treatments present many side effects as a consequence of their poor bioavailability and the need to use higher doses. In view of the exposed evidences, the encapsulation and selective delivery to the diseased tissues of the different therapeutic compounds seem highly convenient. In this sense, silica-based mesoporous nanoparticles offer great loading capacity within their pores, the possibility of modifying the surface to target the particles to the malignant areas and great biocompatibility. This manuscript is intended to be a comprehensive review of the available literature on complex bone diseases treated with silica-based mesoporous nanoparticles, whose further development and eventual translation into the clinic could bring significant benefits for our future society.