Person: Morales Gómez, Paloma
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First Name
Paloma
Last Name
Morales Gómez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Nutrición y Ciencia de los Alimentos
Area
Nutrición y Bromatología
Identifiers
4 results
Search Results
Now showing 1 - 4 of 4
Item Validation of coffee silverskin extract as a food ingredient by the analysis of cytotoxicity and genotoxicity(Food Research International journal, 2017) Iriondo De Hond, Amaia; Haza Duaso, Ana Isabel; Alicia Ávalos; María Dolores del Castillo; Morales Gómez, PalomaThe aim of the present study was to validate the food safety of CSE, by studying its effect on cytotoxicity (100–20000 μg/ml) and genotoxicity (10, 100 and 1000 μg/ml) and also to investigate its preventive potential (1, 10 and 100 μg/ml) against B(a)P induced DNA damage. Prior to analyses, the antioxidant capacity and the microbiological quality of CSE were tested. DNA damage (strand breaks and oxidized purines/pyrimidines) was evaluated by the alkaline single-cell gel electrophoresis or comet assay. HepG2 cells were pre-treated with CSE (1, 10 and 100 μg/ml) for 24 h followed by the addition of 100 μM B(a)P in presence of CSE for other 24 h. Detection of oxidized purines and pyrimidines was carried out using Formamidopyrimidine DNA glycosylase or Endonuclease III enzymes, respectively. Chlorogenic acid (CGA), the major antioxidant present in coffee, was used as a control. Treatment with 100 μM B(a)P significantly increased (p< 0.05) levels of DNA strand breaks and oxidized purine and pyrimidine bases. Treatment of HepG2 cells with CSE did not induce either cytotoxicity or genotoxicity. CSE significantly inhibited (p< 0.05) genotoxicity induced by B(a)P and the observed effect may be associated to its antioxidant capacity. CGA alone at the concentration present in CSE was effective against B(a)P. Thus, CGA seems to be a contributor to the preventive effect of CSE against B(a)P induced DNA damage in HepG2 cells. In conclusion, CSE presents potential as a natural sustainable chemoprotective agent against the chemical carcinogen B(a)P.Item Manufactured silver and gold nanoparticles-induced apoptosis by caspase-pathway in human cell lines(Toxicological and Environmental Chemistry, 2019) Alicia Ávalos; Morales Gómez, Paloma; Haza Duaso, Ana IsabelMetallic nanoparticles have emerged as an important class of nanomaterials for a wide range of industrial and medical applications. Because of the intensive commercial applications, risk assessment of these nanoparticles is of great importance. In the present study, the human hepatoma and leukemia cells were used to characterize the apoptotic effects of silver nanoparticles (4.7 and 42 nm) and gold nanoparticles (30 nm). Apoptotic cells were identified by chromatin condensation and flow cytometry analysis, using Annexin V/PI, TUNEL and caspase activation assays. Flow cytometry analysis showed that the three metallic nanoparticles induced apoptotic cell death in a concentration and time dependent-manner. Moreover, the three nanoparticles induced activation of caspase-3 and -7 in hepatoma and leukemia cells. Apoptotic effects were stronger after exposure of both cell lines with 4.7 nm silver nanoparticles than those obtained with 42 nm silver and 30 nm gold nanoparticles. In conclusion, silver (4.7 and 42 nm) and gold (30 nm) nanoparticles induced apoptosis in hepatoma and leukemia cells via the caspase dependent pathway. The smaller silver nanoparticles (4.7 nm) had a greater ability to induce apoptosis in both cell lines.Item Propiedades funcionales de Hongos Comestibles(Agro Sur, 2019) P. Fernández; Haza Duaso, Ana Isabel; Morales Gómez, PalomaLos hongos superiores o setas han sido recolectados y consumidos por el ser humano durante miles de años, tanto por sus cualidades nutritivas como medicinales. Actualmente, los hongos son consumidos en todo el mundo por su excelente sabor, aroma y textura, aunque es poco conocido su gran potencial como alimentos con propiedades funcionales. Por ello, el objetivo principal de este trabajo ha sido realizar una revisión bibliográfica para identificar los géneros y especies de hongos silvestres y cultivados con propiedades funcionales, así como los compuestos bioactivos o farmacológicos responsables de dichas propiedades. Para ello, se ha obtenido información de diversas bases de datos como PubMed, Google Académico, Web of Science o Scielo, entre otras. También se han realizado consultas de libros externos. Tras la revisión bibliográfica de este trabajo hemos identificado gran cantidad de géneros y especies de hongos que contienen compuestos bioactivos como los β-glucanos o complejos polisacárido-proteína, responsables de las actividades funcionales. Caben destacar entre otras las propiedades antitumorales, inmunomoduladoras, antioxidantes y antimicrobianas. De los 82 artículos científicos revisados, podemos concluir que únicamente 4 hongos presentan la mayoría de las propiedades funcionales descritas. Dos de estos hongos pertenecen al género Agaricus, el Agaricus bisporus y el Agaricus blazei (Champiñones) y los otros dos son el Ganoderma lucidum (Reishi) y la Lentinula edodes (Shiitake)".Item In vitro and in vivo genotoxicity assessment of gold nanoparticles of different sizes by comet and SMART assays(Food and Chemical Toxicology, 2018) Ávalos, A.; Haza Duaso, Ana Isabel; Mateo, D.; Morales Gómez, PalomaDue to the increasing use of gold nanoparticles (AuNPs) in different areas such as medicine, biotechnology or food sector, human exposure to them has grown significantly and its toxicity evaluation has become essential. Therefore, the purpose of this study was to compare the potential genotoxic effects of 30, 50 and 90 nm AuNPs, using in vitro comet assay with the in vivo mutagenic and recombinogenic activity (SMART Test) in Drosophila. The results indicated that in both cell lines, 30, 50 and 90 nm (1–10 μg ml−1) AuNPs increased DNA strand breaks following 24 h treatment. This damage was not dose and size-dependent. Moreover, a modified comet assay using endonuclease III and formamidopyrimidine-DNA glycosylase restriction enzymes showed that in both cell lines, pyrimidines and purines were oxidatively damaged by all AuNPs, being 90 nm AuNPs slightly more genotoxic. However, the data obtained with SMART showed that 30 nm AuNPs did not modify the spontaneous frequencies of spots indicating lack of mutagenic and recombinogenic activity. Therefore, further experiments must be carried out to gain a better understanding of the mechanism of action of AuNPs to ensure their safe use.