Person: Morales Gómez, Paloma
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First Name
Paloma
Last Name
Morales Gómez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Nutrición y Ciencia de los Alimentos
Area
Nutrición y Bromatología
Identifiers
14 results
Search Results
Now showing 1 - 10 of 14
- Project number: 93
Item Propuesta de mejora de la asignatura TFG del Grado en Nutrición Humana y Diétetica. Implantación de la prueba ECOE(2019) Hurtado Moreno, Olivia; Calle Purón, María Elisa; Cuadrado Vives, María Carmen; Morales Gómez, Paloma; Lafuente Duarte, María Esther; Hierro Paredes, Eva; López Gallardo, Meritxell; Redondo Cuenca, Araceli; Villarino Marín, Antonio Luis; Matallana González, María Cruz; Calle Pascual, Alfonso Luis Item Differential Apoptotic Effects of Bee Product Mixtures on Normal and Cancer Hepatic Cells(Antioxidants, 2023) Sánchez Martín, Vanesa; Morales Gómez, Paloma; Iriondo De Hond, Amaia; Fernández Hospital, Xavier; Fernández Álvarez, Manuela; Hierro Paredes, Eva; Haza Duaso, Ana Isabel; Gan, Ren-YouMost effective anticancer drugs normally generate considerable cytotoxicity in normal cells; therefore, the preferential activation of apoptosis in cancer cells and the reduction of toxicity in normal cells is a great challenge in cancer research. Natural products with selective anticancer properties used as complementary medicine can help to achieve this goal. The aim of the present study was to analyze the effect of the addition of bee products [propolis (PR) or royal jelly (RJ) or propolis and royal jelly (PR+RJ), 2–10%] to thyme (TH) and chestnut honeys (CH) on the differential anticancer properties, mainly the cytotoxic and pro-apoptotic effects, in normal and cancer hepatic cells. The cytotoxic effects of samples were analyzed using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (0–250 mg/mL) and the effects on apoptosis were analyzed using cell cycle analysis, TdT-dUTP terminal nick-end labeling (TUNEL) assay, DR5 (Death Receptor 5) and BAX (BCL-2-Associated X) activation, and caspases 8, 9, and 3 activities. Both honey samples alone and honey mixtures had no or very little apoptotic effect on normal cells. Antioxidant honey mixtures enhanced the apoptotic capacity of the corresponding honey alone via both extrinsic and intrinsic pathways. Of all the samples, chestnut honey enriched with 10% royal jelly and 10% propolis (sample 14, CH+10RJ+10PR) showed the highest apoptotic effect on tumor liver cells. The enrichment of monofloral honey with bee products could be used together with conventional anticancer treatments as a dietary supplement without side effects. On the other hand, it could be included in the diet as a natural sweetener with high added value.Item Bee products as an alternative for the preservation of nitrate and nitrite-reduced dry fermented sausages(Food Bioscience, 2024) Fernández Hospital, Xavier; Hierro Paredes, Eva; Martín Cabrejas, Izaskun; Caballero, Natalia; Jiménez Begoña; Sánchez Martín, Vanesa; Morales Gómez, Paloma; Haza Duaso, Ana Isabel; Fernández Álvarez, ManuelaFood regulations are becoming increasingly restrictive on the use of nitrate and nitrite as additives in meat products, so different alternatives are being investigated to replace their functions. In this study, we tested the antioxidant activity of mixtures of bee products to partially replace nitrate and nitrite in dry fermented sausages. Two combinations of chestnut honey, propolis and royal jelly were added to sausages at 2% concentration, together with 0 and 75 mg/kg of nitrate and nitrite. TBARs and volatile profile analysis revealed a lower intensity of oxidation phenomena in sausages prepared with bee products, but these differences were not perceived by the tasters in the sensory analysis. Only instrumental differences in the a* colour parameter were observed when nitrate and nitrite were not used in the formulation. The addition of bee products did not affect the typical microbiota (lactic acid bacteria and gram-positive catalase-positive cocci) of sausages. The combination of 2% bee products and 75 mg/kg of nitrate and nitrite may be useful to obtain dry fermented sausages with a sensory quality and oxidation stability similar to a standard product containing the maximum amounts of these additives allowed in the European Union (150 mg/kg each).Item Validation of coffee silverskin extract as a food ingredient by the analysis of cytotoxicity and genotoxicity(Food Research International journal, 2017) Iriondo De Hond, Amaia; Haza Duaso, Ana Isabel; Alicia Ávalos; María Dolores del Castillo; Morales Gómez, PalomaThe aim of the present study was to validate the food safety of CSE, by studying its effect on cytotoxicity (100–20000 μg/ml) and genotoxicity (10, 100 and 1000 μg/ml) and also to investigate its preventive potential (1, 10 and 100 μg/ml) against B(a)P induced DNA damage. Prior to analyses, the antioxidant capacity and the microbiological quality of CSE were tested. DNA damage (strand breaks and oxidized purines/pyrimidines) was evaluated by the alkaline single-cell gel electrophoresis or comet assay. HepG2 cells were pre-treated with CSE (1, 10 and 100 μg/ml) for 24 h followed by the addition of 100 μM B(a)P in presence of CSE for other 24 h. Detection of oxidized purines and pyrimidines was carried out using Formamidopyrimidine DNA glycosylase or Endonuclease III enzymes, respectively. Chlorogenic acid (CGA), the major antioxidant present in coffee, was used as a control. Treatment with 100 μM B(a)P significantly increased (p< 0.05) levels of DNA strand breaks and oxidized purine and pyrimidine bases. Treatment of HepG2 cells with CSE did not induce either cytotoxicity or genotoxicity. CSE significantly inhibited (p< 0.05) genotoxicity induced by B(a)P and the observed effect may be associated to its antioxidant capacity. CGA alone at the concentration present in CSE was effective against B(a)P. Thus, CGA seems to be a contributor to the preventive effect of CSE against B(a)P induced DNA damage in HepG2 cells. In conclusion, CSE presents potential as a natural sustainable chemoprotective agent against the chemical carcinogen B(a)P.Item Manufactured silver and gold nanoparticles-induced apoptosis by caspase-pathway in human cell lines(Toxicological and Environmental Chemistry, 2019) Alicia Ávalos; Morales Gómez, Paloma; Haza Duaso, Ana IsabelMetallic nanoparticles have emerged as an important class of nanomaterials for a wide range of industrial and medical applications. Because of the intensive commercial applications, risk assessment of these nanoparticles is of great importance. In the present study, the human hepatoma and leukemia cells were used to characterize the apoptotic effects of silver nanoparticles (4.7 and 42 nm) and gold nanoparticles (30 nm). Apoptotic cells were identified by chromatin condensation and flow cytometry analysis, using Annexin V/PI, TUNEL and caspase activation assays. Flow cytometry analysis showed that the three metallic nanoparticles induced apoptotic cell death in a concentration and time dependent-manner. Moreover, the three nanoparticles induced activation of caspase-3 and -7 in hepatoma and leukemia cells. Apoptotic effects were stronger after exposure of both cell lines with 4.7 nm silver nanoparticles than those obtained with 42 nm silver and 30 nm gold nanoparticles. In conclusion, silver (4.7 and 42 nm) and gold (30 nm) nanoparticles induced apoptosis in hepatoma and leukemia cells via the caspase dependent pathway. The smaller silver nanoparticles (4.7 nm) had a greater ability to induce apoptosis in both cell lines.Item Enhancement of the Antioxidant Capacity of Thyme and Chestnut Honey by Addition of Bee Products(Foods, 2022) Sánchez Martín, Vanesa; Morales Gómez, Paloma; Gonzalez-Porto, Amelia V.; Iriondo De Hond, Amaia; Lopez-Parra, Marta B.; Del Castillo, Maria Dolores; Fernández Hospital, Xavier; Fernández Álvarez, Manuela; Hierro Paredes, Eva; Haza Duaso, Ana IsabelHoney consumption and imports have increased in recent years, and it is considered by consumers to be a healthy alternative to more commonly used sweeteners. Honey contains a mixture of polyphenols and antioxidant compounds, and the botanical origin and geographical area of collection play an important role in its chemical composition. The present study investigated the physicochemical properties, total phenolic content, and antioxidant capacity of Spanish thyme honey and chestnut honey, and their mixtures with royal jelly (2% and 10%) and propolis (2% and 10%). The analysis of the physicochemical parameters of both honey samples showed values within the established limits. Propolis showed the highest value of total phenolic content (17.21–266.83 mg GAE/100 g) and antioxidant capacity (DPPH, ORAC, and ABTS assays; 0.63–24.10 µg eq. Tx/g, 1.61–40.82 µg eq. Tx/g, and 1.89–68.54 µg eq. Tx/g, respectively), and significantly reduced ROS production in human hepatoma cells. In addition, mixtures of honey with 10% of propolis improved the results obtained with natural honey, increasing the value of total phenolic content and antioxidant capacity. A significant positive correlation was observed between total phenolic compounds and antioxidant capacity. Therefore, the antioxidant capacity could be attributed to the phenolic compounds present in the samples, at least partially. In conclusion, our results indicated that thyme and chestnut honey supplemented with propolis can be an excellent natural source of antioxidants and could be incorporated as a potential food ingredient with biological properties of technological interest, added as a preservative. Moreover, these mixtures could be used as natural sweeteners enriched in antioxidants and other bioactive compounds.Item Propiedades funcionales de Hongos Comestibles(Agro Sur, 2019) P. Fernández; Haza Duaso, Ana Isabel; Morales Gómez, PalomaLos hongos superiores o setas han sido recolectados y consumidos por el ser humano durante miles de años, tanto por sus cualidades nutritivas como medicinales. Actualmente, los hongos son consumidos en todo el mundo por su excelente sabor, aroma y textura, aunque es poco conocido su gran potencial como alimentos con propiedades funcionales. Por ello, el objetivo principal de este trabajo ha sido realizar una revisión bibliográfica para identificar los géneros y especies de hongos silvestres y cultivados con propiedades funcionales, así como los compuestos bioactivos o farmacológicos responsables de dichas propiedades. Para ello, se ha obtenido información de diversas bases de datos como PubMed, Google Académico, Web of Science o Scielo, entre otras. También se han realizado consultas de libros externos. Tras la revisión bibliográfica de este trabajo hemos identificado gran cantidad de géneros y especies de hongos que contienen compuestos bioactivos como los β-glucanos o complejos polisacárido-proteína, responsables de las actividades funcionales. Caben destacar entre otras las propiedades antitumorales, inmunomoduladoras, antioxidantes y antimicrobianas. De los 82 artículos científicos revisados, podemos concluir que únicamente 4 hongos presentan la mayoría de las propiedades funcionales descritas. Dos de estos hongos pertenecen al género Agaricus, el Agaricus bisporus y el Agaricus blazei (Champiñones) y los otros dos son el Ganoderma lucidum (Reishi) y la Lentinula edodes (Shiitake)".- Project number: 321
Item Integra y aprende. Construyendo una cadena de bloques (blockchain) de la granja a la mesa(2023) Cambero Rodríguez, María Isabel; Aguado Ramo, Juan Antonio; Aguilar Jaime, María Victoria; Alba Rubio, Claudio; Alonso Monge, Rebeca María del Mar; Aragón Ramirez, Alberto; Arias López, Patricia; Arias Revenga, Jorge; Bermudez González, Guillermo; Bermejo Poza, Rubén; Blanch Rojo, María; Blanco Flores, María Dolores; Blanco Montoro, Rafael José; Bonel Ayuso, Diego Paul; Borrero Del Pino, Juan; Bugeda de Bonilla, Inés; Burgía Domínguez, Angélica; Cabeza Briales, María Concepción; Cabezas Albéniz, Almudena; Calahorra Fernández, Felipe José; Castro Madrigal, Teresa; Castro Navarro, Irma; Cervantes Navarro, Isabel; Corugedo Fernández, Lucía; Cruces Díaz, Ainhoa; Díaz Formoso, Lara; Díez Romera, Mariano; Duarete Pacheco, Sofía; Fernández Álvarez, Leonides; Fernández Solís, Claudia; Fernández-Acero Bascones, Teresa; Ferreira García-Osorio, Andrea; Fraga Perucha, Nerea; Fuente Vázquez, Jesús De La; Galicia Larrea, Paula; Gamonal Martos, Miriam; García Álvarez, Andrés; García Balboa, María Del Camino; García Calvo, Eduardo Rafael; García García, Aina; García Lacarra, Teresa; García Quiroga, Sara; García Huch, Uma Jade; González González, Noelia; González Jiménez, Lucía; Haza Duaso, Ana Isabel; Herranz Domingo, Andrea; Herranz Sorribes, Carmen; Isabel Redondo, Beatriz; Jara Pérez, Josué; Jurado Escobar, Rubén; Justo Ruiz, Carolina; Lafuente Orte, Irene; lópez, Cindy Alejandra; López Bote, Clemente José; López Valdeolivas, Patricia; Magro Arconada, Paula; Mallavia León, Blanca; Martín Amores, Ruth; Martín De Santos, María Del Rosario; Morales Gómez, Paloma; Moreda de Figueroa, Blanca; Moreno Conde, Helena María; Muñoz Atienza, Estefanía; Olivares Moreno, Álvaro; Peña Vidal, Nuria; Pérez Cabal, María De Los Ángeles; Pérez Sen, Raquel; Prieto Suárez, María Cinta; Paniagua Roas, Alejandra; Ramis Cervantes, Ana María; Recamal Pagán, Carlota; Remiro Yagüe, Víctor; Rodríguez Fernández, Carmina; Rodríguez Gómez, Santiago; Rodríguez Peña, José Manuel; Romera Villena, Natalia; Salazar Hijosa, Raúl; Sanabria Dominguez, Nerea; Santacruz Parra, Marta; Santos Arnaiz, Carlos; Santos López, Sergio; Torrecilla Velasco, Jose Santiago; Velasco De Diego, Raquel; Velasco Villar, Susana; Villanueva Suarez, María José Item In vitro and in vivo genotoxicity assessment of gold nanoparticles of different sizes by comet and SMART assays(Food and Chemical Toxicology, 2018) Ávalos, A.; Haza Duaso, Ana Isabel; Mateo, D.; Morales Gómez, PalomaDue to the increasing use of gold nanoparticles (AuNPs) in different areas such as medicine, biotechnology or food sector, human exposure to them has grown significantly and its toxicity evaluation has become essential. Therefore, the purpose of this study was to compare the potential genotoxic effects of 30, 50 and 90 nm AuNPs, using in vitro comet assay with the in vivo mutagenic and recombinogenic activity (SMART Test) in Drosophila. The results indicated that in both cell lines, 30, 50 and 90 nm (1–10 μg ml−1) AuNPs increased DNA strand breaks following 24 h treatment. This damage was not dose and size-dependent. Moreover, a modified comet assay using endonuclease III and formamidopyrimidine-DNA glycosylase restriction enzymes showed that in both cell lines, pyrimidines and purines were oxidatively damaged by all AuNPs, being 90 nm AuNPs slightly more genotoxic. However, the data obtained with SMART showed that 30 nm AuNPs did not modify the spontaneous frequencies of spots indicating lack of mutagenic and recombinogenic activity. Therefore, further experiments must be carried out to gain a better understanding of the mechanism of action of AuNPs to ensure their safe use.Item Protective Effect of Thyme and Chestnut Honeys Enriched with Bee Products against Benzo(a)pyrene-Induced DNA Damage(International Journal of Environmental Research and Public Health, 2022) Sánchez Martín, Vanesa; Haza Duaso, Ana Isabel; Iriondo De Hond, Amaia; Del Castillo, María Dolores; Fernández Hospital, Xavier; Fernández Álvarez, Manuela; Hierro Paredes, Eva; Morales Gómez, Paloma; Tchounwou, Paul B.The aim of the present study was to validate the cytotoxicity, genotoxicity, and preventive potential against benzo(a)pyrene (BaP)-induced DNA damage of nine samples of thyme and chestnut honeys enriched with bee products (royal jelly and propolis, 2–10%). Cell viability was determined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (0–250 mg/mL) to select nontoxic concentrations, and DNA damage (0.1–10 μg/mL) was evaluated by the alkaline single-cell gel electrophoresis or comet assay. Treatment with honey samples or royal jelly and propolis did not affect the viability of HepG2 cells up to 100 and 50 mg/mL, respectively. Treatment with 100 μM BaP significantly increased (p ≤ 0.001) the levels of the DNA strand breaks. None of the tested concentrations (0.1–10 μg/mL) of the honey samples (thyme and chestnut), royal jelly, and propolis caused DNA damage per se. All tested samples at all the concentrations used decreased the genotoxic effect of BaP. In addition, all mixtures of thyme or chestnut honeys with royal jelly or propolis showed a greater protective effect against BaP than the samples alone, being the thyme and chestnut honey samples enriched with 10% royal jelly and 10% propolis the most effective (70.4% and 69.4%, respectively). The observed protective effect may be associated with the phenolic content and antioxidant capacity of the studied samples. In conclusion, the thyme and chestnut honey samples enriched with bee products present potential as natural chemoprotective agents against the chemical carcinogen BaP.