Person:
Rodríguez Peña, José Manuel

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First Name
José Manuel
Last Name
Rodríguez Peña
URI
https://hdl.handle.net/20.500.14352/77543
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Microbiología y Parasitología
Area
Microbiología
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Author: Nombela, César × Subject: 576 ×

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    Signalling through the yeast MAPK Cell Wall Integrity pathway controls P-body assembly upon cell wall stress.
    (Scientific reports, 2019) García, Raúl; Pulido, Verónica; Orellana Muñoz, Sara; Nombela, César; Vázquez de Aldana, Carlos R.; Rodríguez Peña, José Manuel; Arroyo, Javier
    Post-transcriptional control of mRNA is a key event in the regulation of gene expression. From yeast to human cells, P-bodies are cytoplasmic RNA-protein aggregates that play an essential role in this process, particularly under stress conditions. In this work, we show that in the model yeast Saccharomyces cerevisiae cell wall stress induces the formation of these structures. This effect is dependent on multiple elements in the Cell Wall Integrity (CWI) MAPK signalling pathway, a signal transduction cascade responsible for the maintenance of cell integrity under adverse environmental conditions. Remarkably, P-body assembly requires the catalytic activity of the MAPK of the pathway, Slt2/Mpk1. In accordance with the control exerted by this signalling pathway, the timing of P-body formation is similar to that of the activation of the CWI pathway. Noticeably, mRNAs whose expression is regulated by this pathway localize in P-bodies after the cell is exposed to stress following a temporal pattern coincident with CWI pathway activation. Moreover, when these mRNAs are overexpressed in a mutant background unable to form visible P-bodies, the cells show hypersensitivity to agents that interfere with cell wall integrity, supporting that they play a role in the mRNA lifecycle under stress conditions.