Person:
Martínez Sainz, María Del Pilar

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First Name
María Del Pilar
Last Name
Martínez Sainz
URI
https://hdl.handle.net/20.500.14352/80635
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Anatomía y Embriología
Area
Anatomía y Anatomía Patológica Comparadas
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    COX-2 is involved in vascular oxidative stress and endothelial dysfunction of renal interlobar arteries from obese Zucker rats
    (Free Radical Biology and Medicine, 2015) Muñoz Picos, Mercedes; Sánchez Pina, Ana Alejandra; Martínez Sainz, María Del Pilar; Benedito Castellote, Sara; López-Oliva Muñoz, María Elvira; García Sacristán, Albino; Hernández Rodríguez, Medardo Vicente; Prieto Ocejo, Dolores
    Obesity is related to vascular dysfunction through inflammation and oxidative stress and it has been identified as a risk factor for chronic renal disease. In the present study, we assessed the specific relationships among reactive oxygen species (ROS), cyclooxygenase2 (COX-2), and endothelial dysfunction in renal interlobar arteries from a genetic model of obesity/insulin resistance, the obese Zucker rats (OZR). Relaxations to acetylcholine(ACh) were significantly reduced in renal arteries from OZR compared to their counterpart, the lean Zucker rat (LZR), suggesting endothelial dysfunction. Blockade of COX with indomethacin and with the selective blocker of COX-2 restored the relaxations to ACh in obese rats. Selective blockade of the TXA2/PGH2 (TP) receptor enhanced ACh relaxations only in OZR, while inhibition of the prostacyclin (PGI2) receptor (IP) enhanced basal tone and inhibited ACh vasodilator responses only in LZR. Basal production of superoxide was increased in arteries of OZR and involved NADPH and xanthine oxidase activation and NOS uncoupling. Under conditions of NOS blockade, ACh induced vasoconstriction and increased ROS generation that were augmented in arteries from OZRandbluntedbyCOX-2 inhibition and by the ROS scavenger tempol. Hydrogen peroxide (H2O2) evoked both endothelium-and vascular smooth muscle (VSM)-dependent contractions, as well as ROS generation that was reduced by COX-2 inhibition.In addition, COX-2 expression was enhanced in both VSM and endothelium of renal arteries from OZR. These results suggest that increased COX-2-dependent vasoconstriction contributes to renal endothelial dysfunction through enhanced (ROS) generation in obesity.COX-2 activity is in turn upregulated by ROS.