Person: Pérez Sancho, Marta
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First Name
Marta
Last Name
Pérez Sancho
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
Identifiers
14 results
Search Results
Now showing 1 - 10 of 14
Item Evaluation of a new enzyme-linked immunosorbent assay for the diagnosis of tuberculosis in goat milk(Research in Veterinary Science, 2020) Roy, A.; Infantes-Lorenzo, J.A.; Dominguez, M.; Moreno, I.; Pérez Sancho, Marta; García Benzaquén, Nerea; García-Seco Romero, María Teresa; Álvarez Sánchez, Julio; Romero Martínez, Beatriz; Gortázar, C.; Juan Ferré, Lucía De; Domínguez Rodríguez, Lucas José; Bezos Garrido, JavierCaprine tuberculosis (TB) is a zoonosis with sanitary and economic repercussions. Caprine TB control programs are based on a test and cull strategy using the intradermal tuberculin tests and slaughterhouse surveillance. However, this approach is not always feasible and may have a limited sensitivity under specific circumstances. In this study, performance of a new experimental test based on the P22 protein complex (P22 ELISA) was evaluated in two TB-infected herds using milk and serum samples and compared with cell-based diagnostic tests. Samples from a low (n = 62, herd 1) and a high (n = 52, herd 2) TB prevalence herd were selected. Moreover, bulk tank milk samples from both herds were analysed using the P22 ELISA. At the end of the study, a group of animals (n = 21) was euthanized and subjected to post-mortem analysis and bacteriological culture. Significant differences (p < .001) on the qualitative and quantitative (ODs) results were observed between herds using both serum and milk samples in the P22 ELISA. The correlation observed in the quantitative results obtained in serum and milk samples was very strong in animals from flock 2 (rs = 0.91) and moderate in animals from flock 1 (rs = 0.46). Among the slaughtered animals, the P22 ELISA detected a higher proportion of lesion-culture positive animals than cell-based diagnostic tests (61.9 and 66.7% using milk and serum samples, respectively). The P22 ELISA using milk samples demonstrated a similar sensitivity compared with serum samples, suggesting it might be a valuable test for TB control in dairy goats.- Project number: 219
Item Infequus: plataforma de enfermedades infecciosas equinas(2019) Juan Ferré, Lucía De; Briones Dieste, Víctor; Bezos Garrido, Javier; Fores Jackson, Paloma; Camino Gutiérrez, Eliazar; Buendía Andrés, Aranzazu; Dorrego Rodríguez, Abel; Cruz López, Fátima; González Domínguez, Sergio; Romero Martínez, Beatriz; García Benzaquén, Nerea; Bárcena Asensio, Carmen; Mazariegos Martínez-Peñalver, María; Ancochea Nodal, Carlos; Hernández Carrillo, Javier; Pérez Sancho, Marta; Pérez Sancho, MartaDesarrollo de la herramienta de formación online Infequus, que ofrecerá a los alumnos y profesionales información actualizada sobre el diagnóstico y control de las enfermedades infecciosas en la especie equina. - Project number: 371
Item Infequus 2.0: Plataforma de enfermedades infecciosas en équidos(2022) Juan Ferré, Lucía De; Cruz López, Fátima; Álvarez Sánchez, Julio; Ancochea Nodal, Carlos; Bezos Garrido, Javier; Briones Dieste, Víctor; García Benzaquén, Nerea; González Domínguez, Sergio; Hernández Carrillo, Javier; Pérez Sancho, Marta; Rodríguez Bertos, Antonio Manuel; Romero Martínez, Beatriz; Santiago Llorente, Isabel; Domínguez Rodríguez, Lucas José Item Environment and Offspring Surveillance in Porcine Brucellosis(Frontiers in Veterinary Science, 2022) Rebollada Merino, Agustín Miguel; Pérez Sancho, Marta; Rodríguez Bertos, Antonio Manuel; García Benzaquén, Nerea; Martínez Alares, Irene; Navarro Gómez, Alejandro; Domínguez Rodríguez, Lucas José; García-Seco Romero, María TeresaPorcine brucellosis, caused by Brucella suis (B. suis), is a notifiable disease causing significant economic losses in production systems.Most infected pigsmay act as carriers and shed B. suis even if asymptomatic. This can contribute to environmental persistence, thus hindering control efforts. Here, the environment and the offspring were investigated during and after a B. suis outbreak at a sow breeding farm. The diagnosis of B. suis in sows (n = 1,140) was performed by culture and polymerase chain reaction (PCR) from vaginal swabs, indirect enzyme-linked immunosorbent assay (I-ELISA) from sera, and brucellin skin test (BST). B. suis diagnosis in post-weaning pigs (n = 899) was performed by I-ELISA in sera and BST. The environmental surveillance programme was implemented by placing gauze sponges (n = 175) pre-hydrated in a surfactant and inactivating liquid for Brucella DNA detection by PCR in different farm areas. Our results showed that the offspring of infected sows reacted to in vivo techniques for B. suis. Furthermore, the offspring born during the outbreak displayed higher seropositivity (I- ELISA) and reactivity (BST) than those pigs born after. Brucella DNA was detected in pregnant sow areas, boxes, boots, and post-weaning pig areas. In addition, Brucella DNA environmental detection was higher during the B. suis outbreak than the post B. suis outbreak. The environmental approach has proven to be a simple, practical, valuable, and safe method to detect and monitor B. suis. These results suggest a role of the environment and the offspring that should be considered in porcine brucellosis surveillance and control programmes.Item Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis(BioMed research international, 2014) Menshawy, Ahmed M S; Hosein, Hosein I; García Benzaquén, Nerea; Martínez Alares, Irene; Sayour, Ashraf E; Goyache Goñi, Joaquín; Azzam, Ragab A A; Álvarez Sánchez, Julio; Domínguez Rodríguez, Lucas José; Pérez Sancho, Marta; García-Seco Romero, María Teresa; Pérez Sancho, MartaBrucellosis is endemic in most parts of Egypt, where it is caused mainly by Brucella melitensis biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. Knowledge of the predominant Brucella species/strains circulating in a region is a prerequisite of a brucellosis control strategy. For this reason a study aiming at the evaluation of the phenotypic and genetic heterogeneity of a panel of 17 Brucella spp. isolates recovered from domestic ruminants (cattle, buffalo, sheep, and goat) from four governorates during a period of five years (2002-2007) was carried out using microbiological tests and molecular biology techniques (PCR, MLVA-15, and sequencing). Thirteen strains were identified as B. melitensis biovar 3 while all phenotypic and genetic techniques classified the remaining isolates as B. abortus (n = 2) and B. suis biovar 1 (n = 2). MLVA-15 yielded a high discriminatory power (h = 0.801), indicating a high genetic diversity among the B. melitensis strains circulating among domestic ruminants in Egypt. This is the first report of the isolation of B. suis from cattle in Egypt which, coupled with the finding of B. abortus, suggests a potential role of livestock as reservoirs of several zoonotic Brucella species in the region.- Project number: 324
Item BIOSLab 2.0: la bioseguridad más allá del laboratorio(2021) Pérez Sancho, Marta; Álvarez Sánchez, Julio; Bezos Garrido, Javier; Domínguez Rodríguez, Lucas José; García Benzaquén, Nerea; García-Seco Romero, María Teresa; González Domínguez, Sergio; Juan Ferré, Lucía De; Mazariegos Martínez-Peñalver, MaríaComplementación y actualización de la plataforma BIOSLab de formación on line en bioseguridad con material didáctico básico para la comunidad universitaria en el actual escenario de pandemia, y especializado para alumnos y profesionales de la salud. Item Management of an outbreak of brucellosis due to B. melitensis in dairy cattle in Spain(Research in Veterinary Science, 2011) Álvarez Sánchez, Julio; Sáez, Jose Luis; García Benzaquén, Nerea; Serrat, Carles; Pérez Sancho, Marta; González Domínguez, Sergio; Ortega, Maria Jesús; Gou, Josep; Carbajo, Lucio; Garrido, Fulgencio; Goyache Goñi, Joaquín; Domínguez Rodríguez, Lucas JoséBrucella melitensis is a major human and animal pathogen, with a wide host range that includes all domestic ruminant species, although small ruminants are its preferred hosts. Outbreaks in cattle due to B. melitensis have become a worldwide emerging problem particularly difficult to control due to the lack of knowledge on the epidemiology in this host species and of an effective vaccine. However, combination of molecular tools and strict biosecurity measures can help to solve these difficulties and eventually eradicate the disease from infected herds. In the present report, management of an outbreak in Spain involving four farms, more than 2000 cattle and several human cases is described. Application of Multiple Locus VNTR Analysis (MLVA) allowed identifying the most likely source of infection. Stamping out and test-and-slaughter strategies were applied, proving their usefulness to control the outbreak depending on infection level, and without the need of other alternative measures.Item Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples(Research in Veterinary Science, 2013) Pérez Sancho, Marta; García-Seco Romero, María Teresa; Arrogante, L; García, N; García Benzaquén, Nerea; Martínez Alares, Irene; Díez Guerrier, Alberto Antoine; Perales, A; Goyache Goñi, Joaquín; Domínguez Rodríguez, Lucas José; Álvarez Sánchez, JulioDNA-based methods have emerged as an additional tool for Brucella infection-confirmation at a herd level. However, their implementation may require the use of specialized equipment. In this context the recently developed loop-mediated isothermal amplification (LAMP) technique may constitute an additional and cost-effective tool for rapid and specific DNA detection, especially in low income areas. In the present study the usefulness of a newly developed LAMP assay aiming at the multicopy-IS711 sequence was assessed on a variety of clinical samples (n = 81 from abortions and ewes; cattle, n = 3; swine, n = 4) that were analyzed in parallel using real-time PCR and bacteriology. Although overall sensitivities obtained with the three methods were comparable (p > 0.05), our results highlighted the complementarity between bacteriology and molecular-based methods for increased sensitivity. Significant differences (p < 0.05) were observed with all techniques depending on the nature of the sample. Our results demonstrate the potential of the IS711-LAMP technique for direct Brucella detection.Item Interferon-gamma responses in sheep exposed to virulent and attenuated Brucella melitensis strains(Veterinary Immunology and Immunopathology, 2014) Pérez Sancho, Marta; Durán-Ferrer, Manuel; García-Seco Romero, María Teresa; Macías, Paula; García Benzaquén, Nerea; Martínez Alares, Irene; Ruiz, Elena; Legaz, Emilio; Díez Guerrier, Alberto Antoine; González Domínguez, Sergio; Domínguez Rodríguez, Lucas José; Álvarez Sánchez, JulioAntibody detection is the basis of large-scale sheep brucellosis diagnosis because of its sensitivity and specificity. In contrast, information on the cellular mediated immune (CMI) response triggered after Brucella melitensis infection, a cornerstone in the protection against this pathogen, is more limited, particularly regarding the effect of the virulence of the infecting strain in the induced CMI reaction. Here, the interferon-gamma (IFN-gamma) profiles evoked after exposure by different routes to virulent (H38) and attenuated (Rev.1) B. melitensis strains in 14 pregnant sheep and 87 ewe lambs, respectively, were characterized accounting for different host-related factors, and compared with their serological response and with the basal IFN-gamma responses observed in 155 animals non exposed to Brucella. No significant differences in the IFN-gamma response of Rev.1 vaccinated animals depending on the inoculation route was observed, in contrast with their serological results. Response in H38- challenged followed a similar trend although peaked later, and an effect of the abortion on the IFN-gamma response was detected. This information could help to understand the interaction bacteria–host that leads to its intracellular survival and could be useful for the design of new diagnostic approaches.Item Time, temperature and media: the three keys to improve the recovery of Campylobacter fetus subsp. venerealis from preputial bull samples(Veterinary Research Communications, 2024) Coral Polo; García-Seco Romero, María Teresa; García Benzaquén, Nerea; Víctor Fernández; Briones Dieste, Víctor; Díez Guerrier, Alberto Antoine; Álvarez Sánchez, Julio; Domínguez Rodríguez, Lucas José; Pérez Sancho, MartaThe isolation of Campylobacter fetus subsp. venerealis (Cfv) from clinical samples is the gold standard for confirming cases of bovine genital campylobacteriosis, an important cause of infertility in cattle and a potential public health concern. Furthermore, isolation is also necessary for the development of autologous vaccines, characterization of strains for antimicrobial susceptibility patterns, etc. Nevertheless, the sensitivity of culture methods is usually low, and there is no standardized protocol to maximize the recovery of Cfv from clinical samples. The aim of the current study is to design a protocol for the culture of Cfv from preputial samples by evaluating the combination of different transport, enrichment and culture media considering the impact of certain factors (time between collection and enrichment, temperature, and use of filters). The use of modified Lander's transport medium and storing the sample for 24 h at 21 ± 2 °C led to the highest recovery of Cfv CFUs. In contrast, the storage of the samples during 24-48 h in PBS and Thomann rarely allowed the recovery of Cfv regardless of the temperature. The enrichment medium yielding the best results was Preston (significantly higher recovery than Brucella medium), while Cfv could not be isolated with Bolton. Regarding our diagnostic assay (using Lander as transport medium and Preston as enrichment medium), the best protocol in terms of maximizing Cfv recovery as well as limiting contaminations is to culture the samples in i) solid media Preston or Skirrow, and ii) using 0.65 μm filters and incubating plates at 37 °C in microaerophilic conditions.