Person: GutiƩrrez Cepeda, Luna
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First Name
Luna
Last Name
GutiƩrrez Cepeda
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Medicina y CirugĆa Animal
Area
Medicina y CirugĆa Animal
Identifiers
8 results
Search Results
Now showing 1 - 8 of 8
Item Simple and economic colloidal centrifugation protocols may be incorporated into the clinical equine sperm processing procedure(Animal Reproduction Science, 2011) GutiĆ©rrez Cepeda, Luna; FernĆ”ndez, A; Crespo CastejĆ³n, Francisco; GosĆ”lvez Berenguer, Jaime; Serres Dalmau, MarĆa ConsolacionFor many years in human assisted-reproduction procedures there have been special protocols to prepare and improve sperm quality. Colloidal centrifugation (CC) is a useful technique that has been proved to enhance semen quality by selection of the best spermatozoa for different species. Its use is recommended to improve fertility of subfertile stallions but current CC protocols are clinically complicated in the equine sperm processing technique due to economic and technical difficulties. The aim of this study was to determine the optimal processing procedures to adapt the use of a CC product (EquiPureā¢) in the equine reproduction industry. A total of nineteen ejaculates were collected from 10 Purebred Spanish Horses (P.R.E horses) using a Missouri artificial vagina. Gel-free semen aliquots were analyzed prior to treatment (control). Semen was subjected to one of six CC protocols with EquiPureā¢ and centrifuged samples were statistically evaluated by ANOVA and Duncan tests (p<0.05) for sperm quality and recovery rate. We obtained higher values by colloidal centrifugation in LIN, STR and BCF variables and DNA fragmentation index trended to be lower in most of the CC protocols. The studied protocols were shown to be as efficient in improving equine sperm quality as the current commercial EquiPureā¢, with the added advantage of being much more economical and simple to use. According to these results it seems to be possible to incorporate single layer and or high colloidal centrifugation volume protocols what would make them simple, economic and clinically viable for the equine sperm processing procedure.- Project number: 44
Item Pintando perros: estudio de la anatomĆa y biomecĆ”nica canina a travĆ©s de la pintura sobre el animal vivo(2017) MartĆnez Sainz, MarĆa Del Pilar; GutiĆ©rrez Cepeda, Luna; Serres Dalmau, MarĆa Consolacion; Contreras RodrĆguez, Julio; Hernandez Fernandez, Tatiana; Gonzalez Hernandez, Inmaculada; GonzĆ”lez MartĆnez, MarĆa EncinaCreaciĆ³n de un material docente (para web y plataformas mĆ³viles) pintando las estructuras anatĆ³micas mĆŗsculo-esquelĆ©ticas sobre perros vivos para el aprendizaje de la AnatomĆa funcional y BiomecĆ”nica canina de forma sencilla, visual y didĆ”ctica. Item OptimizaciĆ³n de las tĆ©cnicas de acondicionamiento del semen equino para los procesos de conservaciĆ³n seminal(2014) GutiĆ©rrez Cepeda, Luna; Serres Dalmau, ConsueloDesde hace muchos aƱos en las tĆ©cnicas de reproducciĆ³n asistida en medicina humana existen protocolos de procesamiento de semen para incrementar su calidad. En la industria reproductiva equina, las tĆ©cnicas de inseminaciĆ³n artificial se han desarrollado ampliamente, aunque el uso de semen criopreservado es menor que en otras especies debido a problemas de supervivencia espermĆ”tica y variabilidad individual. Esta variabilidad individual tambiĆ©n ha sido establecida entre razas. En general, sĆ³lo el 20-Āā30% de los sementales producen un semen con buena capacidad de congelaciĆ³n, ābuenos congeladoresā, otro 40-Āā 60% aproximadamente con capacidad aceptable (aunque se ve afectado negativamente por la criopreservaciĆ³n) y otro 20-Āā30% de sementales producen semen que no es congelable en absoluto, āmalos congeladoresā, Estas limitaciones determinan que la inseminaciĆ³n artificial con semen congelado en el Ć©quido estĆ© menos extendida que en otras especies y encontrar sistemas y procedimientos que permitan incrementar el nĆŗmero de sementales y eyaculados adecuados para refrigeraciĆ³n y/o congelaciĆ³n es una de las metas principales. Unos de los procedimientos mĆ”s extendidos para intentar mejorar la calidad seminal en equinos, especialmente en individuos tipificados como "malos refrigeradores-congeladores", es la eliminaciĆ³n de parte del plasma seminal mediante centrifugaciĆ³n simple. Sin embargo, esta tĆ©cnica no ejerce tareas de selecciĆ³n, sino que concentra tanto los espermatozoides considerados normales, anormales y los no viables en el āpelletā resultante... [ABSTRACT]For many years in human assisted-Āāreproduction procedures there have been special protocols to prepare and improve sperm quality. In the equine industry, artificial insemination techniques have been greatly developed, although cryopreserved semen is still less used comparing to others species due to spermatic survival problems and individual variability. This individual variability has also been established between breeds. In general, 20-Āā30% of stallions are considered to have a good frozen-Āāthawed sperm quality āGood Freezersā, 40-Āā60% average frozen-Āāthawed quality and the other 20-Āā30% are not suitable for cryopreservation āBad Freezersā. All these limitations determine that artificial insemination with frozen semen in the equine is less widespread than in other species and finding systems and procedures to increase the number of stallions and ejaculates suitable to be cooled and cryopreserved is and important goal. It has been suggested that equine sperm quality could be enhanced, particularly in stallions identified as ābad coolers/freezersā, by removing some of the seminal plasma by simple centrifugation. However, such procedure does not select the best spermatozoa as all sperm and debris are presented in the resulting pellet...- Project number: 350
Item Pintando caballos: Estudio de la AnatomĆa y BiomecĆ”nica Equina a travĆ©s de la Pintura sobre el Animal Vivo(2016) MartĆnez Sainz, MarĆa Del Pilar; Rojo Salvador, ConcepciĆ³n; Contreras RodrĆguez, Julio; MarĆn GarcĆa, MarĆa Del Pilar; Serres Dalmau, MarĆa Consolacion; Fores Jackson, Paloma; Re, Michela Tatiana; Andres Alonso, Felipe; GutiĆ©rrez Cepeda, LunaCreaciĆ³n de un material docente (para web y plataformas mĆ³viles) pintando las estructuras anatĆ³micas mĆŗsculo-esquelĆ©ticas sobre caballos vivos para el aprendizaje de la anatomĆa funcional y biomecĆ”nica equina de forma sencilla, visual y didĆ”ctica. - Project number: 376
Item CreaciĆ³n de un entorno de enseƱanza online para los programas de posgrado(2016) Lorenzo GonzĆ”lez, Pedro Luis; FernĆ”ndez Ćlvarez, Manuela; Olmeda GarcĆa, Ćngeles Sonia; Serres Dalmau, MarĆa Consolacion; GutiĆ©rrez Cepeda, Luna; Manzano Barea, ElenaEn este proyecto se ha conseguido modernizar, a travĆ©s de las diferentes labores y actividades desarrolladas, las herramientas de aprendizaje a travĆ©s del uso de medios en formato online que facilitan la comprensiĆ³n y adquisiciĆ³n de conocimientos. Se ha logrado introducir un mĆ©todo alternativo a la enseƱanza tradicional, basado en el aprendizaje online con apoyo de material fĆ”cilmente accesible en web, lo que da la oportunidad a los estudiantes, antiguos alumnos y profesorado de mejorar sus conocimientos, mantenerse actualizados y administrarse sus tiempos de trabajo y estudio. Las herramientas utilizadas han logrado incrementar el nĆŗmero de alumnos matriculados. Este curso acadĆ©mico 2016-2017, el tĆtulo propio de Experto en Bases de la Fisioterapia y RehabilitaciĆ³n Animal, cuenta con el mayor nĆŗmero de matriculaciones desde su inicio en 2012. AdemĆ”s, se ha incrementado el acceso de alumnos extranjeros comunitarios y extracomunitarios, lo que demuestra el alcance de las labores realizadas. Se ha logrado crear una herramienta que favorece la cooperaciĆ³n interdisciplinar en el intercambio de conocimientos entre los diferentes programas de formaciĆ³n de posgrado y entre las diferentes ediciones de los mismos. Se han creado herramientas que han facilitado el uso del inglĆ©s asĆ como las interpelaciones de los alumnos entre si y con los diferentes profesores de la formaciĆ³n solventando las barreras geogrĆ”ficas y creando una comunidad formativa e interactiva de gran utilidad. Item Colloidal centrifugation of stallion semen results in a reduced rate of sperm DNA fragmentation(Reproduction Domestic Animals, 2013) GosĆ”lvez Berenguer, Jaime; Crespo CastejĆ³n, Francisco; GutiĆ©rrez Cepeda, Luna; Serres Dalmau, MarĆa Consolacion; Jonhnston, StephenStallion spermatozoa recovered and examined immediatelyafter colloidal centrifugation resulted in a higher straight-linevelocity (VSL) than sperm processed using direct conven-tional centrifugation (p = 0.000), but there was nodifferences in the progressive motility or sperm DNAfragmentation (SDF) as determined by the sperm chromatindispersion assay. However, when centrifuged spermatozoawere incubated at 37Ā°C for 24 h to determine the rate of SDF(r-SDF), a lower r-SDF (p = 0.0011) was observed in thosesperm recovered after colloidal separation (0.5 Ā± 0.1%āh)compared to direct (1.2 Ā± 0.4%āh) or no centrifugation (r-SDF = 1.2 Ā± 0.3%āh). These results confirm that colloidalseparation of stallion spermatozoa results in prolonged spermDNA longevity, but these differences were only apparentfollowing a period of incubation and dynamic assessment.Consequently, we strongly recommend the use of thedynamic form of the SDF assay for evaluating centrifugationandāor otherex vivoprocedures, as a single basal assessmentof SDF may inadvertently result in a false-positive evaluationof DNA quality.Item Electromyographic and ultrasonographic evaluation of neuromuscular electrical stimulation training on the equine rectus abdominis muscle(Comparative Exercise Physiology, 2019) HernĆ”ndez-FernĆ”ndez, Tatiana; GutiĆ©rrez Cepeda, Luna; LĆ³pez-SanromĆ”n, Javier; Manso DĆaz, Gabriel; Cediel Algovia, RafaelThe current study aimed to determine the effects of neuromuscular electrical stimulation (NMES) on equine rectus abdominis using surface electromyography (sEMG) and ultrasonographic muscle thickness evaluation. Five horses were trained with NMES for 12 weeks; muscle thickness and sEMG evaluations were obtained before and after the training period. Three different tests were carried out for sEMG evaluations: Test A tried to elicit a voluntary maximal isometric contraction (VMIC); Test B used NMES to elicit a muscular contraction; and Test C used 1 ms repetitive electrical impulses to elicit a series of M-waves. Muscle strength was evaluated from the root mean square (RMS; Tests A and B) and peak to peak (PP; Test C) values obtained from the sEMG data. Measures related to amplitude (RMSa; RMSm; RMSa; PP) were normalised with their pre-training values for every muscle prior to statistical analysis, leading to values as a proportion of the initial value. The evaluation of muscle fatigue was performed using a Fourier test analysis of the frequency range, obtaining the median frequency (MF) for all tests. Muscle thickness (MT) was measured by ultrasound of left and right sides of the rectus abdominis. Data were analysed using non parametric test of Wilcoxon (Test A RMSa; Test A RMSm; Test B RMSa; Test B RMSm; Test C PP; MT; P<0.05) and t-test (Test A MF; Test B MF; Test C MF; P<0.05). Statistical differences were observed between baseline and trained horses. Muscle force increase following NMES training in Test B (RMSa 2.50Ā±0.69; RMSm 2.59Ā±0.76) and Test C (PP 5.20Ā±1.76). Fatigue of the rectus abdominis decreased in Test A (168.33Ā±55.19 vs 232.63Ā±44.15 Hz) and Test C MF (187.93Ā±20.76 vs 236.98Ā±52.39 Hz), but not in Test B (363.98Ā±45.48 vs 327.95Ā±50.84 Hz). The difference in muscle thickness between the two groups was not significant (10.96Ā±0.64; 11.78Ā±0.79 mm). The results suggest that NMES training could be used as an effective method to increase muscle force and fatigue resistance of the rectus abdominis muscle in the horse.Item The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders on the fragmentation dynamics of thawed equine sperm DNA(Acta Veterinaria ScandinĆ”vica, 2012) GutiĆ©rrez Cepeda, Luna; FernĆ”ndez, Alvaro; Crespo CastejĆ³n, Francisco; RamĆrez, Miguel Ćngel; GosĆ”lvez Berenguer, Jaime; Serres Dalmau, MarĆa ConsolacionVariability among stallions in terms of semen cryopreservation quality renders it difficult to arrive at a standardized cryopreservation method. Different extenders and processing techniques (such us colloidal centrifugation) are used in order to optimize post-thaw sperm quality. Sperm chromatin integrity analysis is an effective tool for assessing such quality. The aim of the present study was to compare the effect of two single layer colloidal centrifugation protocols (prior to cryopreservation) in combination with three commercial freezing extenders on the post-thaw chromatin integrity of equine sperm samples at different post-thaw incubation (37Ā°C) times (i.e., their DNA fragmentation dynamics). Results Post-thaw DNA fragmentation levels in semen samples subjected to either of the colloidal centrifugation protocols were significantly lower (p<0.05) immediately after thawing and after 4 h of incubation at 37Ā°C compared to samples that underwent standard (control) centrifugation. The use of InraFreezeĀ® extender was associated with significantly less DNA fragmentation than the use of Botu-CrioĀ® extender at 6 h of incubation, and than the use of either Botu-CrioĀ® or GentĀ® extender at 24 h of incubation (p<0.05). Conclusions These results suggest that single layer colloidal centrifugation performed with extended or raw semen prior to cryopreservation reduces DNA fragmentation during the first four hours after thawing. Further studies are needed to determine the influence of freezing extenders on equine sperm DNA fragmentation dynamics.